Seedling method for tissue culture and rapid breeding of camellia semiserrata chi

A technology of safflower camellia and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seed setting rate, small camellia oleifera, slow production, etc., and achieve good seedling quality, fast flowering and fruit setting , the effect of excellent oil quality

Inactive Publication Date: 2012-04-11
张桂琴 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the wide distribution range of ordinary camellia oleifera, the fruit of camellia oleifera is small, the seed setting rate is low, the oil yield is low, and it contains more undesirable substances such as polyphenols and erucic acid; the traditional propagati...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Harvesting explants: Cut off the tender branches of the century-old tea tree of Daguo Honghua Camellia oleifera in Luoman Village, Tianping Town, Tengxian County, Guangxi, and harvest the buds and leaves to keep them fresh. Select the buds and wash them with clean water, and then wash them with laundry Rinse with powdered water, then rinse with running water.

[0023] (2) Aseptic differentiation: use the tea tree buds that have been rinsed clean as explants, disinfect them with 72% to 75% alcohol on the aseptic inoculation room workbench for 10 to 15 seconds, and rinse them with sterile water for 2 to 15 seconds. After 5 times, sterilize in 0.1% to 0.2% mercuric chloride for 8 to 15 minutes, then rinse with sterile water for 4 to 6 times to remove surface debris. Put the sterilized buds under an electron microscope with a power of more than 150 times, use a scalpel to cut off the foreign matter on the surface, clean up the foreign matter on the surface, and use an i...

Embodiment 2

[0032] (1) Harvested explants: Cut off the tender branches of the century-old tea tree of Daguo Honghua Camellia oleifera in Luoman Village, Tianping Town, Teng County, Guangxi, and harvest the buds and leaves to keep them fresh. Select the young leaves and wash them with clean water, and then wash them with laundry Rinse with powdered water, then rinse with running water.

[0033] (2) Aseptic differentiation: use the rinsed tender leaves of Camellia oleifera as explants, disinfect with 72% to 75% alcohol for 10 seconds on the workbench of the aseptic inoculation room, and rinse with sterile water for 2 to 5 times , placed in 0.1% mercury liter for disinfection for 5 to 15 minutes, and then rinsed with sterile water for 4 to 6 times. Put the sterilized leaves under an electron microscope with a power of more than 150 times, cut the 0.3-0.5mm tissue at the lower half of the tip of the young leaves with a scalpel, inoculate them on the differentiation medium and cultivate them t...

Embodiment 3

[0042] (1) Harvesting explants: select the tender branches of the large-fruited safflower oil tea tree planted in Jiangxi Province to cut off, and the buds and leaves are kept fresh and kept fresh, and the buds are selected to be rinsed with clean water, then washed with washing powder water, and then used Rinse with running water.

[0043] (2) Aseptic differentiation: use the tea tree buds that have been rinsed clean as explants, disinfect them with 71% to 76% alcohol for 15 seconds on the aseptic inoculation room workbench, and rinse them with sterile water for 2 to 5 times Put it in 0.2% mercuric chloride for disinfection for 7-15 minutes, then rinse it with sterile water for 4-6 times to remove surface debris. Put the sterilized buds under an electron microscope with a power of more than 150 times, cut off the foreign matter on the surface with a scalpel, clean up the foreign matter on the surface, inoculate the 0.3mm tissue of the bud tip on the differentiation medium wit...

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PUM

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Abstract

The present invention discloses a seedling method for tissue culture and rapid breeding of camellia semiserrata chi. The method comprises: explant collecting, aseptic differentiation, strong seedling culturing and bagged seedling transplanting, wherein the explant is provided for breeding. According to the method, bud tip or leaf is adopted as the explant for the breeding, and is subjected to treatments of disinfection and impurity removing; a differentiation culture media is adopted to culture the treated bud tip or the treated leaf to form a small plant; the induced small plant is cut, and inoculated in a new differentiation culture media to carry out multiple subcultures; the resulting small plant is inoculated on the specially-made strong seedling culture media to carry out culture; finally the bagged seedling transplanting is performed. According to the present invention, the original culture media component is adopted; the culture media adopts the oriented polypropylene film bagas the culture dish; the tea seedlings breed rapidly; a lot of plant viruses can be removed, such that the original fine varietal characters can be maintained; the seedling has characteristics of good quality, high transplanting survival rate, rapid flowering, rapid fruiting, high oil yield, high oil quality and long high yield period; the method of the present invention has characteristics of strong operability, high application value and originality, and provides a new approach for the camellia oleifera seedling breeding, wherein the approach has characteristics of rapid breeding and rapid commissioning.

Description

technical field [0001] The invention relates to a rare edible vegetable oil seedling breeding method, in particular to a seedling raising method for tissue culture and rapid propagation of Camellia oleifera. Background technique [0002] Camellia oleifera belongs to the Theaceae family Camellia genus. It is an evergreen small tree that blooms from December to March of the next year with large flowers and fruits. It is an excellent tree species for the production of edible oil, ornamental plants and greening of barren hills; fruit picking in autumn . [0003] Daguo safflower camellia has great economic value, and the whole tree is a treasure. Camellia oleifera fruit is composed of husk, seed shell and seed kernel, the ratio is 63.64:14.38:21.98, the weight of a single fruit is 0.5-1.5 kg, the oil yield of dry seeds is 50-62%, the leaves of the trees are large and thick, evergreen, Conducive to water conservation, protection of the ecological environment. The husk can be us...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张桂琴冯志祥邱先强黄雪娟
Owner 张桂琴
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