Composite microbial agent capable of degrading kitchen waste at normal temperature, its preparation method and its application
A technology for compounding microbial inoculants and kitchen waste, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to solve humidity, poor effect, large odor, etc., and achieve stable treatment effect. simple steps
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Embodiment 1
[0022] Example 1 A Composite Microbial Agent for Degrading Kitchen Waste at Normal Temperature
[0023] The preparation method is:
[0024] (1) Cultivate bacterial strains and prepare composite bacteria
[0025] First, the lyophilized powder of the strain Lactobacillus acidophilus zsq11 was added to a 5mL liquid medium for shaking-table culture, and gradually enlarged to a 100L fermenter functional medium for culture. Cultivate in a fermenter at 28-38°C for 12-24 hours, preferably 16-20 hours, and then collect the cells by centrifugation. The composition of liquid medium and functional medium are: potassium nitrate 1g, sodium chloride 0.5g, yeast extract 0.5g, FeSO 4 ·7H 2 O 0.01g, pH7.2~7.4, when preparing, add water to 1000ml.
[0026] Firstly, the freeze-dried powder of the strain yeast Saccharomyces sp.zsq13 was added to 5ml liquid medium for shaking-table culture, and gradually enlarged to 100L fermenter functional medium for culture. Cultivate in a fermenter at 28-3...
Embodiment 2
[0038] Example 2 A Composite Microbial Agent for Degrading Kitchen Waste at Normal Temperature
[0039] The preparation method of the composite microbial bacterial agent of this embodiment is except that the weight ratio of the Lactobacillus acidophilus zsq11 thallus and the yeast Saccharomyces sp.zsq13 thallus obtained by centrifugation in step (1) is 1.5: 1; Broken peanut shells: sand: shavings: bran=2: 1.5: 0.8: 2; in step (3), except that the mass percentage of the compound thallus accounting for the compound microbial inoculum is 6%, other steps are all the same as in the examples 1 is the same.
[0040] The composite microbial bacterial agent of embodiment 2 is to the degradation rate test of kitchen waste:
[0041] (1) The composition of kitchen waste: the leftover meals in the canteen, remove the large and hard parts such as bones;
[0042] (2) Put the compound microbial bacterial agent that embodiment 1 makes into garbage machine, after continuing to ferment for 24h...
Embodiment 3
[0048] Example 3 A Composite Microbial Agent for Degrading Kitchen Waste at Normal Temperature
[0049] The preparation method of the composite microbial bacterial agent of this embodiment is except that the weight ratio of the Lactobacillus acidophilus zsq11 thallus and the yeast Saccharomyces sp.zsq13 thallus that centrifuges in the step (1) is 2: 3; Broken peanut shells: sand: wood shavings: bran=2.5: 1: 1: 2; in step (3), except that the mass percentage of the compound thallus accounting for the compound microbial bacterial agent is 8%, other steps are all the same as those in the examples 1 is the same.
[0050] The composite microbial bacterial agent of embodiment 3 is to the degradation rate test of kitchen waste:
[0051] (1) The composition of kitchen waste: the leftover meals in the canteen, remove the large and hard parts such as bones;
[0052] (2) Put the compound microbial bacterial agent that embodiment 1 makes into garbage machine, after continuing to ferment...
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