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Protein capable of promoting chloroplast development and coding gene and application thereof

A technology that encodes genes and proteins, which can be used in applications, genetic engineering, plant genetic improvement, etc., can solve few problems, and achieve the effects of increasing crop yield, improving photosynthetic capacity, and high economic benefits

Inactive Publication Date: 2012-05-30
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The function of this enzyme has been extensively studied in yeast and mammals, but there are few reports on this enzyme in plants

Method used

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  • Protein capable of promoting chloroplast development and coding gene and application thereof
  • Protein capable of promoting chloroplast development and coding gene and application thereof
  • Protein capable of promoting chloroplast development and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, phenotype and genetic analysis of rice white stripe mutant ws

[0058] 1. Phenotype analysis of rice white stripe mutant ws

[0059] The rice white stripe mutant ws is a natural mutant of Zhongxian 3037 (WT, purchased from the Agricultural College of Yangzhou University) found in the field by our laboratory. Compared with the control 3037, the phenotype of the white-striped mutant was that white stripes were distributed on the leaves along the veins from the seedling stage. As the plant grew, the white stripes gradually increased on the leaves, and the most serious phenotype was the second leaf. The phenotype of the sword leaf is somewhat relaxed. At the booting stage, white stripes were distributed on most of the second leaves of the severe phenotype plants. The mutant phenotype does not affect the normal growth and development of rice under normal cultivation conditions ( figure 1 ). The analysis of the chlorophyll content of the leaves of the plant...

Embodiment 2

[0066] Example 2, the acquisition of ribonucleotide reductase small subunit WS and its coding gene WS

[0067] 1. Map-based cloning of WS genome genes

[0068] In order to clone the WS gene, we will use the homozygous white stripe mutant ws to cross with Nipponbare, and the obtained F 1 F 2 Population, among which 180 F2 recessive individuals (F2 generation individuals with white stripe phenotype) were initially mapped for WS gene. Using STS (Sequence-Tagged Site) molecular markers and using PCR method, we found that the STS markers S1, S2, S3 and S4 on chromosome 6 had obvious linkage with the mutation site. Most of the exchanged plants between the mutation site and S2 were also exchanged between the mutation site and S1, and most of the exchanged plants between the mutation site and S3 were included in the mutation site and S4 in exchange among individual plants. At the same time, the exchanged individual plants between the mutation site and S3 were different from those ...

Embodiment 3

[0076] Example 3, Complementation Experiment of White Stripe Mutant WS Phenotype

[0077] 1. Construction of complementary vector pCWS and complementary control vector pCWSC

[0078] BAC OsJNBb0055C04 (purchased from Shanghai National Gene Research Center, Chinese Academy of Sciences, No. OsJNBb0055C04) was digested with EcoR I to obtain 3363 bases upstream of the start codon ATG of WS and 3510 bases after the stop codon TGA. The DNA fragment (7893bp) of the full-length sequence was cloned between the EcoR I recognition sites of pCAMBIA1300 (DingGuo, MCV033), and the complementary expression vector pCWS was constructed. The constructed complementary vector pCWS was digested with PstI to remove part of the coding region of the WS gene and the 5' promoter region of the gene, and retain the part of the coding region and the 3' regulatory region at the 3' end, thus constructing the complementary control vector pCWSC( Figure 4 Middle C).

[0079] 2. Obtaining and phenotypic ident...

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Abstract

The invention discloses a rice protein capable of promoting chloroplast development and a coding gene and application thereof. The protein is one of 1) a protein formed by amino acid residue sequences of the SEQ ID No.2 in a sequence table or 2) a protein which is formed after one or more amino acid residues in the amino acid residue sequences of the SEQ ID No.2 in the sequence table are substituted and / or deleted and / or added and has the same activities as the amino acid residue sequences of the SEQ ID No.2. The gene is used for coding the small subunit of ribonucleotide reductase, and the development of rice chloroplast is affected after the gene is mutated so that white stripes are distributed on the rice leaves in the vein direction.

Description

technical field [0001] The invention relates to a protein for promoting chloroplast development, its coding gene and application. Background technique [0002] Chloroplast is a unique organelle of eukaryotic photosynthetic organisms, and the photosynthesis of eukaryotic photosynthetic organisms is carried out in the chloroplast. Photosynthesis is the only process on earth that can convert solar energy into chemical energy on a large scale, synthesize inorganic matter into organic matter, and release oxygen. It provides food, energy, and oxygen for the life activities of humans, animals, plants, and countless microorganisms. In a nutshell, photosynthesis is the most fundamental reaction to sustain life on Earth. Therefore, the research on the mechanism of chloroplast development will help people understand the regulation mechanism of photosynthesis and make better use of solar energy. A lot of work has been done, many of which have come from the study of mutants with pale g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N7/01C12N15/82
Inventor 程祝宽洪丽兰李明唐丁王克剑
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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