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Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof

A technology of polybrominated diphenyl ethers and degrading bacteria, applied in the field of biological treatment of environmental pollutants

Inactive Publication Date: 2012-07-04
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Pseudomonas stutzeri (Pseudomonas stutzeri) is a common indigenous microorganism in soil, freshwater, seawater and other environments. Patented technologies have been applied to denitrification, phosphorus removal, sulfur removal, degradation of pesticides and polycyclic aromatic hydrocarbons, and antibacterial agents etc., but there is no patented technology to apply the bacteria to the degradation of PBDEs

Method used

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  • Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof
  • Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof
  • Efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and application thereof

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Experimental program
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Effect test

Embodiment 1

[0016]Inoculate the prepared bacterial agent in the inorganic salt medium containing PBDEs (BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183, BDE-209), and the PBDEs in the medium The concentration was controlled in the range of 20-100 μg / L, and the culture was shaken at 150 r / min and 30° C. in the dark. After 14 days, the degradation efficiency was shown in Table 1.

[0017] The degradation efficiency of PBDEs in the culture medium of table 1

[0018]

Embodiment 2

[0019] Embodiment 2: the degradation of PBDEs in the waste water

[0020] The wastewater from a sewage treatment plant has a pH of 5.3 to 8.6, and the types and concentrations of PBDEs are shown in Table 2. Add the bacteria agent of degrading bacteria BFR01 (10 6 ~10 8 CFU / mL), placed at room temperature and cultured in the dark for 10 days, and the residual amount of PBDE was determined. The experimental results are shown in Table 2.

[0021] Table 2 Degradation efficiency of PBDEs in sewage treatment plant wastewater

[0022]

Embodiment 3

[0023] Embodiment 3: the degradation of PBDEs in soil

[0024] The pH value of a PBDEs-contaminated soil is 4.0-10.0, and the types and contents of PBDEs are shown in Table 3. Add 1.0mL of bacterial agent per gram of soil, and the bacterial density in the soil is controlled at 10 6 ~10 8 CFU / g. After 10 days, the content of PBDEs was determined, and the experimental results are shown in Table 3.

[0025] Table 3 Degradation efficiency of PBDEs in soil

[0026]

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Abstract

The invention discloses efficient degradation bacteria for PBDEs (polybrominated diphenyl ethers) and an application thereof. Aiming at the characteristics of poor biochemical perforamnce, common pollution and diverse types of PBDEs, the invention provides a kind of economical, efficient, broad-spectrum and environment-friendly PBDEs degradation bacteria and an application thereof based on the current situation of PBDEs degradation, primarily the application in degrading PBDEs in wastewater biological treatment and environment biological remediation. The efficient degradation bacteria BFR01 provided by the invention come from the soil polluted by PBDEs, and are obtained by manual enrichment and separation and purification. The strain BFR01 is gram negative bacteria which have relatively small bacterial colony, white non-transparent round shape and non-smooth surface; the thalli can be spherical or ellipsoidal with diameter of 0.5-0.7 mu m; catalase and oxidase are positive, and V-P reaction is negative; and the nitrate can be reduced, and glucose can be oxidized to generate acid. In an aerobic condition, the bacteria not only have relatively strong PBDEs degradation ability, but also can degrade PBDEs at broad spectrum, and can be applied to the PBDEs-containing wastewater biological treatment and environment biological remediation.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a high-efficiency polybrominated diphenyl ether-degrading bacterium and its application in wastewater biological treatment and environmental bioremediation. technical background [0002] Polybrominated diphenyl ethers (Polybrominated diphenyl ethers, PBDEs), as a cheap and efficient flame retardant, are widely added to electronic devices, plastics, textiles, rubber, office utensils and other products, depending on the number of bromine atoms on the benzene ring , PBDEs have a total of 209 congeners, tetrabromodiphenyl ether, pentabromodiphenyl ether, hexabromodiphenyl ether and decabromodiphenyl ether are common in environmental media. Since there is no chemical bond between PBDEs and product materials, PBDEs in products will inevitably be released into the environment during the production, transportation, use, and disposal of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/38A62D101/28
Inventor 夏星辉张尚伟郑少奎夏娜赖芸佳
Owner BEIJING NORMAL UNIVERSITY
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