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Method for increasing enzymolysis efficiency of rice straws through choline and amino acid ionic liquid pretreatment

A technology of ionic liquid and rice straw, which is applied in the production and fermentation of bulk chemicals, can solve problems such as unfriendly environment, and achieve the effect of overcoming resource waste, improving yield and realizing utilization.

Inactive Publication Date: 2014-07-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this type of ionic liquid as a lignocellulose pretreatment solvent overcomes the disadvantages of the traditional ionic liquid pretreatment process such as being unfriendly to the environment

Method used

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  • Method for increasing enzymolysis efficiency of rice straws through choline and amino acid ionic liquid pretreatment
  • Method for increasing enzymolysis efficiency of rice straws through choline and amino acid ionic liquid pretreatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] [Choline][Glycine] Pretreatment Improves Enzymatic Hydrolysis Efficiency of Rice Straw

[0024] a) Pretreatment: Accurately weigh 150 mg of rice straw powder (150–350 μm) and 3 g of [choline][glycine] ionic liquid, put them together in a 15 mL Erlenmeyer flask, and store them at 90 °C under nitrogen protection. Stir for 24 hours; then cool to room temperature, add 1 times the volume of ionic liquid NaOH solution (0.1 mol / L) to dilute, filter, and then wash the filter residue with 3 times the volume of deionized water for 5 times, and the filter residue is dried to obtain the pre- Treated rice straw.

[0025] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U Li The commercialized cellulase of Trichoderma spp. was sealed and placed in a constant temperature oscillator at 200 r / min and 50 °C for reaction. 200 μL samples were taken regularly, a...

Embodiment 2

[0029] [Choline][Serine] Pretreatment Improves the Efficiency of Enzymatic Hydrolysis of Rice Straw

[0030] a) Pretreatment: Accurately weigh 150 mg of rice straw powder (150–350 μm) and 3 g of [choline][serine] ionic liquid, put them together in a 15 mL Erlenmeyer flask, and store them at 90 °C under nitrogen protection. Stir for 24 hours; then cool to room temperature, add 2 times the volume of ionic liquid NaOH solution (0.1 mol / L) to dilute, filter, and then wash the filter residue 5 times with 3 times the volume of deionized water, and the filter residue is dried to obtain the pre- Treated rice straw.

[0031]b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U Li The commercialized cellulase of Trichoderma spp. was sealed and placed in a constant temperature oscillator at 200 r / min and 50 °C for reaction. 200 μL samples were taken regularly, ...

Embodiment 3

[0035] [Choline][Methionine] pretreatment improves rice straw enzymatic hydrolysis efficiency

[0036] a) Pretreatment: Accurately weigh 150 mg of rice straw powder (150–350 μm) and 3 g of [choline][methionine] ionic liquid, put them together in a 15 mL Erlenmeyer flask, and store them at 90 °C under nitrogen protection. Stir at low temperature for 24 hours; then cool to room temperature, add 1.5 times the volume of ionic liquid NaOH solution (0.1 mol / L) to dilute, filter, and then wash the filter residue with 3 times the volume of deionized water for 5 times, and the filter residue is obtained after drying. Treated rice straw.

[0037] b) Enzymatic hydrolysis: Weigh 20 mg of the pretreated rice straw accurately, place it in a 50 mL Erlenmeyer flask, add 5 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U Li The commercialized cellulase of Trichoderma spp. was sealed and placed in a constant temperature oscillator at 200 r / min and 50 °C for reaction. 200 μL samples were ta...

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Abstract

The invention discloses a method for increasing enzymolysis efficiency of rice straws through choline and amino acid ionic liquid pretreatment, which comprises the following steps: firstly, performing pretreatment on the rice straws at certain temperature for a certain period of time by taking choline and amino acid ionic liquid as a solvent; then filtering and separating to obtain residues; drying the residues to obtain the pretreated rice straws. The residues of the rice straws are used as substrates, and enzymolysis is performed on the substrates by utilizing cellulase and xylanase to finally obtain sugar liquor taking glucose and xylose as main components. The method for increasing the enzymolysis efficiency of the rice straws through the choline and amino acid ionic liquid pretreatment not only is capable of effectively increasing the enzymolysis efficiency of the rice straws and increasing the yield of reducing sugar (the glucose and the xylose) which can be fermented, but also is capable of overcoming the defects that a traditional ionic liquid pretreatment process environment is not friendly and the like.

Description

technical field [0001] The invention belongs to the field of lignocellulose utilization and chemical industry application, and specifically relates to a method for improving rice straw enzymatic hydrolysis efficiency by pretreatment of [choline][amino acid] ionic liquid. Background technique [0002] With the increasing scarcity of petroleum resources, in recent years, people have gradually shifted their attention to the development and utilization of renewable resources such as biomass. Compared with fossil resources, biomass resources have many advantages. It is not only inexhaustible and inexhaustible, but also can effectively reduce CO 2 emissions and environmental pollution. As the most widely distributed and abundant renewable biomass resource on the earth, lignocellulose has attracted much attention. Lignocellulose is mainly composed of lignin, cellulose and hemicellulose. The reducing sugars obtained by enzymatic hydrolysis of cellulose and hemicellulose, such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
CPCY02P20/54
Inventor 李宁侯雪丹宗敏华
Owner SOUTH CHINA UNIV OF TECH
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