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Application of lactobacillus johnsonii in preparation of feed used for improving livestock immunity level

A technology of Lactobacillus johnsonii and feed, which is applied in the application field of Lactobacillus johnsonii strain in preparing feed for improving the immunity level of livestock and poultry

Active Publication Date: 2012-07-18
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no product in China that uses Lactobacillus johnsonii as an additive to add food and feed additives, and its application in animal and poultry breeding is rarely reported. Lactobacillus johnsonii is developed into an animal microecological feed additive. Filling in the domestic gap and screening Lactobacillus johnsonii with excellent probiotic properties is of great significance to promoting the level of livestock and poultry breeding in my country, and can play a greater role in livestock and poultry breeding

Method used

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  • Application of lactobacillus johnsonii in preparation of feed used for improving livestock immunity level
  • Application of lactobacillus johnsonii in preparation of feed used for improving livestock immunity level
  • Application of lactobacillus johnsonii in preparation of feed used for improving livestock immunity level

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Lactobacillus johnsonii CGMCC No.4926 freeze-dried agent

[0038] 1) Activate cryopreserved Lactobacillus johnsonii CGMCC No.4926 on the MRS agar plate for three times, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, culture it at 37°C for 10 hours, then transfer it to 250mL MRS with 1% inoculum Liquid medium, used as seed liquid after culturing for 10 hours;

[0039] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculum size of 5%;

[0040] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment for 14 hours under the condition of 100rpm, N 2 , to maintain dissolved oxygen less than 1%;

[0041] 4) When the pH of the feed liquid no longer decreases or the absorbance...

Embodiment 2

[0045] (1) Experimental animals and experimental design

[0046] 540 124-day-old Hailan brown commercial layer chickens were purchased from a Beijing layer hen farm. The formula and nutrient composition of the basal diet are shown in Table 1.

[0047] Table 1 Basic diet formula and nutritional components

[0048]

[0049] Note: Each kilogram of feed contains: VA 16mg, VD 3 5mg, VE 20mg, VK 3 3mg, VB 1 4.6mg, VB 2 10.5mg, VB 6 8.6mg, VB 12 2mg, protein powder 390mg, secondary powder 5473.55mg, calcium pantothenate 14.5mg, nicotinamide 40.2mg, folic acid 2.3mg, biotin 10mg, manganese sulfate 251.57mg, zinc sulfate 202.9mg, iron sulfate 199.33mg, copper sulfate 31.92mg, potassium iodide 50mg, sodium selenite 30mg, stone powder 1234.28mg, choline chloride 1000mg, MET 1000mg.

[0050] According to the body weight, 540 healthy laying hens were divided into 6 treatment groups according to the single factor random experiment design, each treatment group had 6 repetiti...

Embodiment 3

[0069] (1) Experimental animals and experimental design

[0070] 540 1-day-old female healthy Jinghong egg chicks were purchased from the hatchery of Beijing Huadu Yukou Poultry Industry Co., Ltd. The formula and nutrient composition of the basic diet are shown in Table 4.

[0071] Table 4 Basic dietary formula and nutritional components

[0072]

[0073]

[0074] They were randomly divided into 6 treatment groups, each treatment group had 6 replicates, and each replicate had 15 chickens. The 1st group is blank control group, feeds basal diet; The 2nd-6 group is Lactobacillus johnsonii test group, adds the 1.0 * 10 that prepares in embodiment 1 respectively in basal diet. 4 , 1.0×10 5 , 1.0×10 6 , 1.0×10 7 , 1.0×10 8 cfu / g of Lactobacillus johnsonii. The test period is 120 days.

[0075] (2) Animal experiment methods

[0076] The experimental chickens were reared in a semi-open chicken house under the same conditions in a 3-layer stepped cage with a feeding dens...

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Abstract

The invention discloses an application of lactobacillus johnsonii CGMCC No.4926 in preparation of feed used for improving livestock immunity level. When the lactobacillus johnsonii is added into the feed in appropriate dosage, blood serum Newcastle disease antibody titer and IgA level of laying hens, laying chicks and broilers can be obviously improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a strain of Lactobacillus johnsonii in preparing feed for improving the immune level of livestock and poultry. Background technique [0002] Lactobacillus johnsonii belongs to Firmicutes, Bacilli, Lactobacillales, Lactobacillaceae, Lactobacillus, and was first isolated in 1980. It has been classified as Lactobacillus acidophilus in science. In 1992, Fujisawa et al. named it Lactobacillus johnsonii through biochemical experiments and DNA hybridization experiments. Lactobacillus johnsoni mainly exists in the digestive tract of animals and humans, and is the dominant flora. [0003] The European Food Safety Authority (EFSA) issued two health declarations against Lactobacillus johnsonii in 2009 and 2011 respectively, stating that Lactobacillus johnsonii has microorganisms that reduce intestinal pathogens, enhance natural defense and immune system and main...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16
Inventor 张军汪攀孙晓雯闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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