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Microbial strain and method for efficiently disposing kitchen refuses

A technology of kitchen waste and microbial preparations, which is applied in the field of kitchen waste treatment and mixed bacterial strains for kitchen waste treatment. It can solve the problems of high oil content, inability to degrade well, high food residue, etc., and achieve the degradation time Short, high degradation rate, efficient degradation effect

Inactive Publication Date: 2012-07-18
上海恒晔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Aiming at the problem of low degradation efficiency of biodegradable kitchen waste in the prior art, the present invention provides a set of kitchen waste treatment microbial preparations with high degradation rate, short degradation time, strong stability and safety, and the use of The method for treating kitchen waste with the microbial preparation is especially aimed at solving the two problems of relatively high oil and fat content in my country's swill waste and that food residues with high fiber content cannot be well degraded.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Weigh 10g of the soil sample for analysis with sterilized weighing paper, add it to a triangular flask filled with 90ml of sterile water (with stones or glass beads inside), shake for 15min, that is 10 -1 concentrated soil suspension. Let stand for 30s, pipette 10 with a 1ml pipette -1 Put 1ml of the concentrated soil suspension into a 9ml sterile water tube, blow and suck 3 times and mix well, which is 10 -2 Concentration of the soil suspension, sequentially from 10 -2 serially diluted to 10 -5 , that is, a series of 10-fold soil dilutions (the operation process is aseptic). Spread soil dilutions of different concentrations on each screening medium plate and incubate at 30°C for 1-6 days. Number the single colonies of different shapes grown on the plate, and purify each strain, and finally transfer the purified strains to a slant for storage. Four strains of Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis and Cellulomonas luteogenes were screened ou...

Embodiment 2

[0050] The four bacterial strains screened in Example 1 were spot-splotted on each enzyme-producing primary screening plate, cultured at 30°C for 1-2 days, observed whether there was a transparent circle around the colony, and measured the ratio of the diameter of the transparent circle to the diameter of the colony to determine The enzyme-producing ability of each strain was semi-quantitatively determined. Casein plate, grease plate and cellulose plate can directly observe the transparent circle; amylase plate needs to be observed after adding Lugol’s iodine solution.

[0051] The enzyme-producing ability of the strains screened is determined by the above-mentioned method, wherein, Bacillus subtilis (protease-producing), Bacillus megaterium (amylase-producing), Bacillus licheniformis (lipase-producing), Cellulomonas lutea (producing cellulase) all have high enzyme-producing ability.

[0052] Streak the four selected strains on the nutrient agar plate according to the combina...

Embodiment 3

[0056] By Bacillus subtilis, Bacillus megaterium, Bacillus licheniformis, Cellulomonas xanthogenes, Bacillus cereus, Bacillus firmus, Bacillus lateral sporosum totally seven groups of strains, according to the method described in embodiment 2 and 2: The ratio of 2:2:2:1:1:1 is prepared into solid microbial mixed strains or preparations, and the swill degradation experiment is carried out according to the method described in Example 2, and the remaining amount after the treatment of the previous day is measured before feeding the next day . The experimental results are as follows:

[0057] Table 1 Experimental results of treating swill with biological preparations of the present invention (unit: kg)

[0058] date sawdust Throw in slop water bacteria Residue after treatment first day 160 50 20 2 the next day 100 200 third day 51 200 fourth day 190

[0059] Total feeding amount=160+50+20+2+100...

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PUM

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Abstract

The invention provides a microbial mixed strain, a microbial preparation and a method for disposing kitchen refuses by using the microbial mixed strain or the microbial preparation. Various microorganisms are screened and separated from soil in the natural world, and the microbial strain capable of efficiently disposing kitchen refuse wastes can be formed after purification, domestication, multi-matching and other steps. After the kitchen refuses are thrown into a biochemical disposer, added with the strain and stirred evenly, kitchen waste source decrement rate reaches above 96%.

Description

technical field [0001] The invention relates to a method for treating kitchen waste by biodegradation and a mixed strain of microorganisms, in particular to a mixed strain for treating kitchen waste and a method for using the mixed strain to treat kitchen waste. Background technique [0002] Kitchen waste (swill) generally refers to food cuttings and food residues from restaurants, hotels and other catering industries, as well as public canteens such as enterprises, institutions, and colleges and universities. With the rapid growth of urban economy and the rapid expansion of urban scale, kitchen waste is increasing day by day. The amount of kitchen waste produced in my country exceeds 20Kt every day. According to reports, the amount of swill generated in Shanghai alone reaches more than 1,300 tons every day. Because kitchen waste is easy to ferment, deteriorate, and rot, it not only produces a large amount of toxins and emits foul-smelling gases, but also pollutes water bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20B09B3/00C12R1/10C12R1/11C12R1/125C12R1/085C12R1/07C12R1/01
CPCY02W30/78
Inventor 卞保华徐静静
Owner 上海恒晔生物科技有限公司
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