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Novel peptide, applications and preparation method thereof

A new type of technology, applied in the field of pharmacy and medicine, can solve the problem of dangerous self-antigen, and achieve the effect of reducing the incidence rate, delaying the disease process, and maintaining weight stability.

Inactive Publication Date: 2012-08-15
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the pathogen invades, the body's immune system will produce a resistant immune response against the pathogen. At this time, the immune response against the bacterial-derived HSP65 endows the body with the ability to resist infection, but at the same time, due to the high homology of human-derived HSP60 and microbial-derived HSP65 molecules , the immune system may respond to its own HSP60 while recognizing bacterial HSP65, and this cross-immune reaction makes HSP65 / 60 molecules a dangerous self-antigen

Method used

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  • Novel peptide, applications and preparation method thereof
  • Novel peptide, applications and preparation method thereof
  • Novel peptide, applications and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the preparation process of novel peptide

[0025] Synthesize a novel peptide whose amino acid sequence is: HGDTTFEYQDIPALDSLTPANED (named IA-2-P2, see SEQNO.1), and its specific flow chart is shown in Figure 4 ; P277 (VLGGGCALLRCIPALDSLTPANED, see SEQ NO.2); P2 (T epitope sequence in P277, IPALDSLTPANED, see SEQ NO.3), the above peptides were all synthesized by Jill Biochemical Shanghai Co., Ltd. using FMOC solid phase synthesis, HPLC Detected purity >90%.

[0026] Its specific preparation method is as follows:

[0027] 1. Resin selection

[0028] Peptides are connected from the C-terminus to the N-terminus, so the synthesis of peptides is reversed in sequence. First select Fmoc-Asp(Otbu)-OH Wang Resin;

[0029] 2. Removal of Fmoc

[0030] Before each next amino acid is connected, the protecting group on the amino group of the previous amino acid must be removed. Add the prepared deprotection solution (20% hexahydropyridine and 80% DMF) into the rea...

Embodiment 2

[0037] Example 2: Pharmacodynamic evaluation of novel peptide IA-2-P2

[0038] 1. Animal immunity

[0039]Sixty 4-week-old female non-obese diabetic (NOD / Lt) mice (purchased from Shanghai Slaccas Experimental Animal Center (Slaccas Experimental Animal), license number: SCXK 2003-0003.) were randomly divided into 5 groups, 12 in each group. Only, respectively: control group (blank control group), placebo group (solvent control group, negative control group), P277 group (positive control group), P2 group (positive control group), IA-2-P2 group. Before administration, the oil preparation is temporarily prepared according to the ratio of 1 mg polypeptide + 40 mg mannitol dissolved in 1 ml 20% Lipofundin. At the age of 4, 7, and 10 weeks, they were injected subcutaneously, three times in total. The drug concentration in the administration group was 1 mg / ml, and the dosage was 80 μg / animal / time, that is, 80 μl liquid medicine; the negative control group: 80 μl oil / animal / time.

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Abstract

The invention provides peptide used for preventing and treating diabetes. According to the weak immunogen of antigen polypeptide, a fragment of antigenic epitope (IPALDSLTPANED) derived from HSP60 is inserted into the downstream of a linear B-epitope sequence HGDTTDEYQD in a membrane closing area of insulinoma associated protein 2 to form the peptide. According to the peptide, the generation times of NOD (nonobese diabetic) mice diabetes can be reduced obviously, so that the novel peptide plays the role of preventing and treating I-type diabetes. Meanwhile, the peptide can be used for transgenic animals and recombinant microorganisms, so that the transgenic animals and the recombinant microorganisms can be used for producing the peptide or preparing oral vaccine for preventing and treating diabetes and related diseases of diabetes.

Description

technical field [0001] The present invention relates to the related fields of pharmacy and medicine, in particular to the diagnosis, prevention and treatment of diabetes; more specifically, the present invention relates to a class of novel peptides, their preparation methods and uses, and compounds containing the novel peptides and their derivatives and analogues. The pharmaceutical preparation can be used for diagnosis, prevention and treatment of human diabetes. Background technique [0002] Diabetes (Diabetes) is caused by genetic factors, immune dysfunction, microbial infection and its toxins, free radical toxins, mental factors and other pathogenic factors acting on the body to cause hypofunction of pancreatic islets and insulin resistance. A series of metabolic disorder syndromes such as fat, water and electrolytes are clinically characterized by hyperglycemia, and typical cases may manifest as polyuria, polydipsia, polyphagia, and weight loss. Diabetes is the second ...

Claims

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Application Information

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IPC IPC(8): C07K14/47A61K38/17A61P3/10
Inventor 刘景晶马艳君吴洁李泰明曹荣月金亮董元楷侯景黄文睿
Owner CHINA PHARM UNIV
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