Microwave assisted plasma sample pre-processing method and application in plasma metabonomics analysis

Abstract

The invention relates to the technical field of analytical chemistry and biological chemistry, the traditional plasma metabonomics sample pre-process is derived for 1 hour at 70 DEG C after oximation for 2 hours at 35 DEG C, the work is heavy, time-consuming and low-efficiency. The invention provides a sample pre-processing method of plasma oximation and derivatization assisted by microwave, which comprises the steps of: adding an oximation reaction reagent to the plasma sample, carrying out the oximation reaction under the condition of low-energy and short-time microwave; adding a derivatization reagent to carry out the derivatization reaction under the condition of high-energy and medium-time microwave. The invention also applies the processing method to the analysis of plasma metabonomics with a gas chromatograph-mass spectrometer. The method in the invention is applied to the process of the plasma treatment, the processing requirement of the sample and the requirement of the metabonomics analysis are met, the pre-processing time is shortened obviously, and the analysis efficiency is improved greatly.

Description

technical field [0001] The invention relates to the technical fields of analytical chemistry and biochemistry, in particular to a microwave-assisted plasma sample pretreatment method and its application in plasma metabolomics analysis. Background technique [0002] Metabonomics (metabonomics / metabolomics) is an emerging discipline developed in the late 1990s. Through the analysis of group indicators, high-throughput detection and data processing are carried out to study the dynamic metabolic changes of the whole body or tissue cell system, especially It is the characteristics and influence of endogenous metabolism, genetic variation, environmental change and even various substances entering the metabolic system. The commonly used analytical techniques are gas chromatography-mass spectrometry (GC / MS), liquid chromatography-mass spectrometry (LC / MS) and nuclear magnetic resonance (NMR). Among them, GC-MS is called the "gold standard" of metabolite analysis in metabolomics res...

AI Technical Summary

Problems solved by technology

Among them, GC-MS is called the "gold standard" of metabolite analysis in metabolomics research (see literature 1: Fischer E, Sauer U. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. European Journal of Biochemistry, 2003, 270 (5): 880-891. See literature 2: Zimmermann D, Hartmann M, Moyer M P, et al. Metabolomics of human colon cell lines. Metabolomics, 2007, 3 (1): 13- 17.), but using GC / MS for analysis, most samples need to be derivatized at room temperature or at elevated temperature. The traditional sample pretreatment conditions for plasma metabolomics are oximation at 35°C for 2 hours and then derivatization at 70°C. Derivatization reaction at ℃ for 1 hour (see literature 3: Jian Weixiong, Yuan Zhaokai, Huang Xianping, et al. Detection and analysis of plasma metabolomics in coronary heart disease with blood stasis syndrome, Chinese Journal of Integrated Traditional Chinese and Western Medicine, 2010, 30(6): 579-584) , which is not only a heavy workload but also time-consuming and inefficient

[0003] It has been reported in the literature that microwaves can promote organic chemical reactions (see literature 4: Fan Xingjun, You Jinmao, Tan Qianzu, etc. Research progress in microwave-promoted organic chemical reactions, Progress in Chemistry, 1998, 10(3): 285-295), using microwave Auxiliary derivatization can effectively shorten the derivatization time, but it has not shortened the longest time-consuming oximation reaction, and microwave-assisted derivatization alone cannot fully improve the efficiency

[0004] At present, there are no literature reports at home and abroad on the method of pretreatment of plasma samples using microwave-assisted oximation and derivatization technology in the study of plasma metabolomics

Images