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Gynura bicolor protein extract, and preparation method and application thereof

A protein extract and a protein extraction technology are applied in the field of red phoenix protein extract and its preparation, which can solve the problems of imperfection and no hypoglycemic effect, and achieve the effect of preventing degradation.

Inactive Publication Date: 2012-09-26
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the hypoglycemic effect of red phoenix is ​​not perfect, the main chemical components isolated from red phoenix are flavonoids, and these flavonoids are kaempferol, quercetin, rutin, isoquercetin Mainly flavonoids and their glycosides, which are common flavonoid chemical components, have no particularly significant hypoglycemic effect

Method used

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  • Gynura bicolor protein extract, and preparation method and application thereof
  • Gynura bicolor protein extract, and preparation method and application thereof
  • Gynura bicolor protein extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] TCA / acetone method to extract red broccoli protein:

[0025] Weigh 10 g of the penultimate 3-4 fresh leaves of the red phoenix plant, wash them twice with tap water, then wash them once with distilled water, add liquid nitrogen and grind them into fine powder, pour them into a 50 ml centrifuge tube, add 30 ml Add 3ml of trichloroacetic acid (TCA) to ice-cooled acetone at -20°C, vortex and mix well, place the mixture at -20°C for 90 minutes, centrifuge at 4°C (10,000 rpm×20 min), discard the supernatant, The obtained precipitate was washed twice with 30 ml of cold acetone, centrifuged, and the precipitate was taken to dry at room temperature, and 6 ml of extraction buffer (8 mol / L urea, 2 mol / L thiourea, 4% (W / V) CHAPS , 0.4% (V / V) amphoteric carrier buffer (Ampholin pH 3.0-9.5, 50 mmol / L DTT), shake and mix, let stand for 5 minutes, centrifuge at 18°C ​​(10,000 rpm×20 min), and take the supernatant , add 8 ml 10% TCA / cold acetone (containing 20 mmol / L DTT), store at -2...

Embodiment 2

[0028] Tris-HCl buffer extraction / (NH 4 ) 2 SO 4 Precipitation method to extract red broccoli protein:

[0029]Take 20 g of the penultimate 3-4 fresh leaves of the red phoenix plant, add 200 ml of pre-cooled extraction buffer (50 mmol / L Tris-HCl pH 8.5, 0.1 mmol / L PMSF, 1% NaCl), homogenate, Extract at 4°C for 20 min, centrifuge (10,000 rpm×15 min), take the supernatant, add (NH 4 ) 2 SO 4 Make the saturation up to 80%, place it at 4°C for 20 min, then centrifuge (10,000 rpm×15 min), and the resulting precipitate is the protein of 0-80% saturation of red phoenix. Add an appropriate amount of 50 mmol / L Tris-HCl (pH 8.5) to the precipitate, and after complete dissolution, put the protein solution into a dialysis bag for dialysis to remove residual (NH 4 ) 2 SO 4 and finally freeze-drying the protein solution to prepare the freeze-dried powder of the red broccoli protein extract.

[0030] 1.55 g sample was obtained by this method, and the protein content was 49.4% by Kj...

Embodiment 3

[0032] Tris-HCl buffer extraction / (NH 4 ) 2 SO 4 Fractional precipitation method to extract red broccoli protein:

[0033] Take 20g of the penultimate 3-4 fresh leaves of the red phoenix plant, add pre-cooled extraction buffer (50 mmol / L Tris-HCl pH 8.5, 0.1 mmol / L PMSF, 1% PVP, 2 mmol / L EDTA-Na 2 , 20 mmol / L DTT, 10 mmol / L Vc, 1% NaCl) 200ml, homogenate, extract at 4°C for 20min, filter through 2 layers of gauze, take the filtrate and centrifuge (10,000 rpm×15min), get the supernatant, and put it on the Added to the supernatant (NH 4 ) 2 SO 4 Make the saturation up to 20%, put it at 4°C for 10min, and then centrifuge (4°C, 10,000 rpm×15min), the obtained precipitate is the protein of 0-20% saturation of the extracted Chinese cabbage; take the supernatant, add (NH 4 ) 2 SO 4 Make the saturation reach 50%, place it at 4°C for 10 minutes, and then centrifuge (4°C, 10,000 rpm×15 min), the obtained precipitate is the protein of 20-50% saturation of the extracted Chinese c...

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PUM

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Abstract

The invention discloses a Gynura bicolor protein extract, and a preparation method and application thereof. The Gynura bicolor protein extract is prepared from fresh leaves of Gynura bicolor by a protein extraction method. The Gynura bicolor protein extract disclosed by the invention has an obvious effect on inhibiting alpha-glucosaccharase, and can be further researched and developed as a healthproduct or medicine for adjuvantly treating diabetes.

Description

technical field [0001] The invention specifically relates to a protein extract of red broccoli and its preparation method and application. Background technique [0002] Hong Feng Cai ( Gynura bicolor DC.) is a perennial herbaceous plant of the genus Panax notoginseng in the Compositae family, mainly distributed in southern my country. Hongfengcai, also known as Xuepicai, Guanyincai, Guanyin amaranth, purple-backed geranium, etc., can be eaten as a vegetable and has a certain hypoglycemic effect. It is cultivated in a small amount in some areas. At present, the research on the hypoglycemic effect of red phoenix is ​​not perfect. The main chemical components isolated from red phoenix are flavonoids. These flavonoids are kaempferol, quercetin, rutin, isoquercetin Mainly flavonoids and their glycosides, which are common flavonoid chemical components, have no particularly significant hypoglycemic effect. Contents of the invention [0003] The purpose of the present ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/30A23L1/29A61K38/02A61P3/10A23L33/00
Inventor 李维林任冰如马丽梁呈元吴菊兰陈剑吕寒
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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