Preparation method of biomedical materials of multiclass functional group rich in amino group, carboxyl group and benzoquinonyl group

A biomedical material and amino-rich technology, which is applied in the field of preparation of biomedical engineering materials with multiple functional groups on the surface, can solve problems such as difficult to achieve co-immobilization of multiple biomolecules, and achieve inhibition of smooth muscle cell adhesion and proliferation and reaction conditions. Mild and easy, the effect of simplifying the fixing conditions

Inactive Publication Date: 2014-11-26
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the organic thin films prepared by the existing technologies usually only contain one kind of functional groups, and it is difficult to realize the co-immobilization of various biomolecules.

Method used

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  • Preparation method of biomedical materials of multiclass functional group rich in amino group, carboxyl group and benzoquinonyl group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing a multifunctional implant device, the steps of which are:

[0016] A. Construction of organic thin films containing multi-type functional groups

[0017] Immerse the implanted device modified with plasma polyallylamine coating in a Tris buffer solution (pH=8.5) of 0.1 mg / ml gallic acid, react for 2 hours, and after fully cleaning, introduce amino groups, carboxyl, quinone.

[0018] B. Co-immobilization of aptamer, bivalirudin and VEGF

[0019] The implanted device containing multiple types of functional groups obtained in step A is soaked in a WSC solution with a concentration of 10 μg / ml polypeptide aptamer, and the WSC solution is composed of the following components: 9.76 mg / ml of 2-(N-morpholine ) ethanesulfonic acid (MES) buffer solution, 1mg / ml of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and 0.24mg / ml of N-hydroxysuccinamide ( NHS). After the reaction is sufficient, rinse fully with PBS (phosphate buffer solution) and distill...

Embodiment 2

[0022] A method for preparing a multifunctional implant device, the steps of which are:

[0023] A. Preparation of organic thin films containing multi-type functional groups

[0024] Prepare a gallic acid-Tris solution with a pH value of 10 and a concentration of 8 mg / ml, add 8 mg / ml decanediamine and a 316L stainless steel sample, react for 12 hours, and after thorough cleaning, a surface containing amino groups, carboxyl groups, Quinone-based organic thin films with multiple functional groups.

[0025] B. Co-immobilization of CD34 antibody and endothelial growth factor (VEGF)

[0026] Soak the multi-type functional group organic film obtained in step A in the buffer solution of WSC (the same components as in Example 1) containing 1 μg / ml CD34 antibody at a pH value of 5, react at room temperature at 20°C for 12 hours, and then use Rinse thoroughly with PBS and distilled water, then soak in PBS buffer solution containing 100ng / ml VEGF with a pH value of 7.4, react at room t...

Embodiment 3

[0029] A method for preparing a multifunctional implant device, the steps of which are:

[0030] A. Preparation of organic thin films containing multi-type functional groups

[0031] Prepare a gallic acid-Tris solution with a pH value of 10 and a concentration of 10 mg / ml, add arginine at 10 mg / ml and use a cobalt-based alloy as a substrate, react for 24 hours, and after thorough cleaning, a surface containing Amino, carboxyl, quinone-based organic films containing multiple types of functional groups.

[0032] B. Co-immobilization of CD34 antibody and heparin

[0033] Soak the multi-type functional group organic film obtained in step B in a buffer solution containing 10 μg / ml CD34 antibody with a pH value of 5 WSC (the components are the same as in Example 1), and react at room temperature for 2 hours at 20° C. Rinse thoroughly with PBS and distilled water as in 7.4. Then soak in the buffer solution of WSC (the same components as in Example 1) containing 1 μg / ml heparin at ...

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Abstract

The invention discloses a preparation method of biomedical materials of a multiclass functional group rich in amino groups, carboxyl groups and benzoquinonyl groups. The multiclass functional group rich in the amino groups, the carboxyl groups and the benzoquinonyl groups is constructed on the surface of a biomedical substrate material, the steps comprise soaking the substrate material in alkalinity Tris buffered solution of gallic acid with the concentration of 10ng / ml-20ng / ml in an environment rich in the amino groups, and reacting for 15min-30days to be sufficiently washed to obtain the biomedical material containing multiple functional groups. The preparation method has the advantages of being simple in operation, soft in reaction condition, easy to apply and controllable in functional group density process. Followed manufactured implantation instruments have good anticoagulation, endothelial cell adhesion, proliferation and migration functions are promoted, smooth muscle cell adhesion and proliferous functions are obviously restrained, and in-situ rapid induction endothelium functionalization of intravascular stents, intravascular stents and tissue engineering scaffolds which induce capillaries to form is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering materials, especially the preparation of biomedical engineering materials with multiple types of functional groups on the surface. Background technique [0002] The unique advantage of surface modification is that it can realize an advanced technology that can selectively enhance the surface properties of the material without changing the overall performance of the material itself. As an important material surface modification technology, biomolecular immobilization has been widely used in the fields of biosensors, medical diagnosis, regenerative medicine, tissue engineering, and biomedical devices. Especially in some cases, such as cardiovascular implants, the surface should have the comprehensive excellent performance of anticoagulation and promoting endothelial repair, which can be achieved through different functional biomolecules (such as: heparin, VEGF, CD34 antibody, adaptati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L33/04A61L33/10A61L33/12A61L31/08A61L31/10
Inventor 王进杨志禄黄楠涂秋芬陈思罗日方
Owner SOUTHWEST JIAOTONG UNIV
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