Preparation method for liquefied strain of edible mushroom

A technology for edible fungi and strains, which is applied in the fields of botanical equipment and methods, applications, and gardening, and achieves the effects of simple inoculation, short production cycle and cost reduction.

Inactive Publication Date: 2012-10-10
浙江常山荣昇食用菌有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing edible fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example one:

[0021] Preparation of Liquefaction Strains of Xiuzhen Mushroom:

[0022] step 1:

[0023] Purification culture of Xiuzhen Mushroom solid strain: select the solid medium composition and weight ratio: cottonseed hull: sawdust: wheat bran: corn flour: gypsum: lime: = 42: 40: 10: 5: 2: 1, add water After the water content reaches 58%~62%, mix well and then bottling (750ml / bottle), sterilize at 0.15Mpa pressure for 2 hours~2.5 hours, cool to below 28℃ in a clean cooling room, under aseptic conditions , Put the mother seed of Xiuzhen mushroom into the solid culture medium, connect 4 to 6 bottles of solid culture medium to a 20mm×200mm female seed, and then move it into the culture room at 23℃~25℃, keep the air humidity at 60%~75% , After 30 to 35 days of ventilating and avoiding light, the bacteria will be cultivated into solid strains of Xiuzhen mushroom;

[0024] Step 2: Preparation of sterile water: use steam sterilization to prepare sterile water, that is, pass s...

Example Embodiment

[0027] Embodiment two:

[0028] Preparation of Flammulina velutipes liquefied strain:

[0029] step 1:

[0030] Purification culture of Flammulina velutipes solid strains: The composition and weight ratio of solid medium are selected as: cotton seed husk: sawdust: wheat bran: corn flour: rice bran: gypsum powder: lime: =50:18:15:10:5:1.5: 0.5. After adding water, the water content reaches 58%~62%, mix well and then bottle (750ml / bottle), sterilize 2 hours~2.5 hours under 0.15Mpa pressure, and cool to below 28℃ in a clean cooling room. Under aseptic conditions, connect the mother species of Flammulina velutipes to the solid culture medium, and then connect 4 to 6 bottles of solid culture medium to a 20mm×200mm mother seed, and then move it into a sterile room at 21℃~23℃ to maintain air humidity After 60% to 75%, ventilated and protected from light for 35 to 40 days, cultivated into solid strains of Flammulina velutipes;

[0031] Step 2:

[0032] Preparation of sterile water: prepare s...

Example Embodiment

[0037] Embodiment three:

[0038] Preparation of liquefied strains of seafood mushrooms:

[0039] Step 1: Purification and cultivation of solid culture of seafood mushrooms: select the solid medium composition and weight ratio: cotton seed hull: sawdust: wheat bran: corn flour: rice bran: gypsum powder = 50:15:15:10:9:1 , After adding water, the water content is 58%~62%, and the mixture is evenly mixed and then bottled (750ml / bottle), sterilized under 0.15Mpa pressure for 2 hours to 2.5 hours, and cooled to below 28℃ in a clean cooling room. Under the condition of bacteria, the mother seeds of seafood mushrooms are connected to the solid medium, a 20mm×200mm mother seed is connected to 4-6 bottles of solid medium, and then transferred to a sterile room at 23℃~25℃ for 35 days~ After 40 days, cultivate into a solid strain of seafood mushroom;

[0040] Step 2: Preparation of sterile water: use a water filter to directly prepare sterile water;

[0041] Step 3: Liquefaction of solid stra...

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Abstract

The invention discloses a preparation method for liquefied strains of edible mushrooms. The preparation method comprises four steps of purification culture of solid strains, sterile water preparation, solid strain liquefaction and liquefied strain inoculation. Under an aseptic condition, the solid strains are added into a crushing machine and are crushed into paste strains, then the sterile water is added for diluting the paste strains to obtain the liquefied strains, and finally the liquefied strains are poured into culture bags to complete the inoculation by an appliance. The preparation method for liquefied strains of edible mushrooms has the advantages of simplified process, short production period, simplicity and convenience in inoculation, rapid spore germination, uniform cell age, early fruiting, good quality, high yield, high efficiency, small pollution risk, small investment and operating cost, good economic benefit and the like.

Description

technical field [0001] The invention belongs to a method for cultivating edible fungus strains, in particular to a preparation method for liquefied edible fungus strains. Background technique [0002] The production of edible fungus strains is a key link in the cultivation of edible fungi. To achieve high-quality and high-efficiency edible fungus cultivation, the production of high-quality, high-efficiency, and low-cost strains must first be achieved. The production of existing edible fungus strains mainly includes two production methods: solid strains and liquid strains. The production process of solid strains is as follows: mother species→original species→cultivated species→bacteria bag (or cultivation bottle) , that is, under aseptic conditions, use a 20mm×200mm parent seed to connect 4 to 6 bottles (750ml / bottle) of solid medium that has been thoroughly sterilized, and culture it at an appropriate temperature to become the original seed; Then use 1 bottle of the origina...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 周国才黄良水
Owner 浙江常山荣昇食用菌有限公司
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