Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology

A technology of eutrophication and polylactic acid, applied in the direction of membrane technology, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of complex use method, complex processing, cumbersome preparation process, etc. The effect of secondary pollution

Active Publication Date: 2012-10-10
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

For example, if inorganic materials such as silica gel and activated carbon are used as immobilization carriers, there are defects such as separation from water and loose bonding between the surface and the immobilized enzyme; while chitosan has defects such as complex processing and unstable properties.
In addition, the preparation process of the existing immobilization materials is cumbersome, the use method is complicated, and certain support materials must be used, which cannot be applied in situ

Method used

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  • Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology
  • Method for eutrophic water pollution treatment through polylactic acid fiber membrane immobilized compound enzyme technology

Examples

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Embodiment 1

[0021] Weigh 25 grams of polylactic acid particles and dissolve them in 20 milliliters of dichloromethane, add 0.5 grams of hydrophilic block copolymer surfactant P123, mix evenly and then stir to obtain a polylactic acid / block copolymer hybrid gel. Weigh α-amylase, hormone-sensitive lipase, papain, fungal cellulase, fungal laccase, soybean urease with a mass ratio of 1.0:15:7.0:4.5:0.25:8.8, and dissolve a total of 500 mg in 2 ml Phosphate buffer solution with a pH value of 6.9, stirred vigorously until completely dissolved, mixed with polylactic acid / block copolymer mixed gel, stirred vigorously and introduced into the spinning device to be spun.

[0022] The target restoration water body samples were collected from an artificial lake in a park in the North Third Ring Road of Beijing. The sampling point was located at the shore of the lake 3 meters, the water depth was 0.5 meters, and the sampling volume was 5 liters. The water quality indicators were: total nitrogen 3.8 mg / L...

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Abstract

The invention relates to a multi-enzyme immobilized electrospun fiber membrane used for treating a eutrophic polluted water body. By using an electrospinning technology, a multi-enzyme system which comprises alpha-amylase, hormone sensitive lipase, papain, fungal cellulose, fungal laccase, soybean urease and the like and can decompose various organic matters causing eutrophic water body pollution is immobilized in a polylactic acid electrospun fiber membrane and directly covers the surface of the eutrophic polluted water body to catalytically degrade various nitrogen-containing organic matters, so as to achieve the goal of reducing the eutrophic pollution in the water body. The polylactic acid electrospun fiber membrane which carries the multi-enzyme system can rapidly and conveniently remove typical eutrophic pollutants in the water body in time to rapidly recover water quality. Compared with other eutrophic pollution treatment techniques, the multi-enzyme immobilized electrospun fiber membrane has the advantages of high efficiency and good performance, can be expected to be used for remediation of urban moderate or severe eutrophic polluted water bodies or in the aquiculture field.

Description

technical field [0001] The invention belongs to the technical field of water pollution control, in particular to a method for preparing and using an immobilized composite enzyme active fiber membrane for treating eutrophication polluted water bodies. Background technique [0002] Water environment protection is a basic national policy of our country. The urban river and lake water system is an important part of the urban environmental system, but in recent years, my country's urban water system has experienced varying degrees of algal blooms year after year, and a large number of algae growing in urban rivers and lakes have brought adverse effects on the ecological environment and reputation of the city . Prevention and control of algae blooms are important measures to protect the urban and rural environment. The outline of the national "Eleventh Five-Year Plan" also clearly stated that comprehensive environmental management and control of eutrophic polluted water bodies are...

Claims

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Application Information

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IPC IPC(8): B01D71/74C02F1/44C02F3/00
CPCY02W10/37
Inventor 牛军峰代云容殷立峰丁士元包月平沈珍瑶
Owner BEIJING NORMAL UNIVERSITY
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