Graphene oxide/hyaluronic acid nanometer drug carrier material, preparation method and application of graphene oxide/hyaluronic acid nanometer drug carrier material

A nano drug carrier, hyaluronic acid technology, applied in the direction of pharmaceutical formulations, medical preparations of non-active ingredients, etc., to achieve good biocompatibility effect

Inactive Publication Date: 2012-10-17
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since its discovery in 2004, graphene has quickly become the most active research frontier in the field of materials science and condensed matter p

Method used

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  • Graphene oxide/hyaluronic acid nanometer drug carrier material, preparation method and application of graphene oxide/hyaluronic acid nanometer drug carrier material
  • Graphene oxide/hyaluronic acid nanometer drug carrier material, preparation method and application of graphene oxide/hyaluronic acid nanometer drug carrier material
  • Graphene oxide/hyaluronic acid nanometer drug carrier material, preparation method and application of graphene oxide/hyaluronic acid nanometer drug carrier material

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Experimental program
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Effect test

Embodiment 1

[0034] (1) Mix 2 grams of natural graphite powder and 60 grams of sodium chloride together and grind them thoroughly. After grinding for 30 minutes, dissolve them in water and filter them with suction;

[0035] (2) After drying the above filter paper, put it into a 100ml round-bottomed flask filled with 23mL of concentrated sulfuric acid, add 0.5g of sodium nitrate at the same time, place it in an ice-water bath at 6°C for magnetic stirring, and after stirring for 30min, slowly Add 3.0 g of potassium permanganate, and continue stirring in a water bath at 10°C for 8 hours;

[0036] (3) Maintain the above reaction (2) Stir at 35°C for 30 minutes, at 65°C for 45 minutes, and at 98°C for 30 minutes;

[0037] (4) After step (3), add 46mL of deionized water, continue to stir for 30min, and then add 200mL of 3% H 2 o 2 Aqueous solution; stop the reaction after stirring for 30min; then ultrasonicate the reaction solution in an ultrasonic machine for 2h;

[0038] (5) The reaction so...

Embodiment 2

[0043] (1) Get the dry graphene oxide that 50mg embodiment 1 makes, be dispersed in aqueous solution according to the concentration of 1mg / mL; According to graphene oxide: EDC: the ratio of NHS mass ratio is 1:5:15 and adds EDC and NHS;

[0044] (2) Add 1 mg / mL NaOH to adjust the pH of the solution to 5.76;

[0045] (3) Add 520 mg of adipic acid hydrazide (ADH), and stir for 24 hours at room temperature;

[0046] (4) Wash several times by centrifugation with deionized water, and freeze-dry for 24 hours to obtain graphene oxide / adipate hydrazide (GO-ADH);

[0047] (5) Weigh 15mg hyaluronic acid and dissolve it in 15mL deionized water;

[0048] (6) Add EDC and NHS respectively according to the mass ratio of hyaluronic acid:EDC:NHS of 2:5:15;

[0049] (7) Use 1 mg / mL NaOH to adjust the pH of the solution to 5.6;

[0050] (8) Add 30 mg of the freeze-dried sample in step (4), and stir for 24 hours at room temperature;

[0051](9) Wash several times by centrifugation with deion...

Embodiment 3

[0055] (1) Prepare different concentrations of DOX, the solvent is DMSO, the concentrations are: 40, 60, 100, 200, 300, 1000, 1500, 2000μg·mL -1 ;

[0056] (2) Weigh 5mg of GO-HA and add to 10mL of the above solution;

[0057] (3) Place in a shaker at 200 rpm, and rotate for 20 hours at 37°C;

[0058] (4) Then wash with 3 mL of water for 3 times, and finally dilute to 25 mL with DMSO;

[0059] (5) Measure the UV absorbance of 8 supernatants with different concentrations, and calculate the drug loading respectively. The results are as follows: Figure 6 shown. It can be seen from the figure that when the concentration of doxorubicin is greater than or equal to 1000 μg·mL -1 When , the drug loading capacity of graphene oxide reaches saturation, about 300mg / g;

[0060] Figure 7 For the drug release of graphene oxide / hyaluronic acid in this example at different pH values, the solution was diluted 12.5 times with PBS before testing. (a): UV-Vis spectra of doxorubicin (DOX) ...

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Abstract

The invention discloses a graphene oxide/hyaluronic acid nanometer drug carrier material. The graphene oxide/hyaluronic acid nanometer drug carrier material is characterized in that adipic acid hydrazide is covalently connected on the surface of monolayer graphene oxide, targeted molecular hyaluronic acid is further coupled by amino groups on the adipic acid hydrazide; the monolayer graphene oxide is 0.5-2nm thick; and the average size of the graphene oxide/hyaluronic acid nanometer drug carrier material is 50-300nm. According to the graphene oxide/hyaluronic acid nanometer drug carrier material provided by the invention, the hyaluronic acid is covalently modified on the surface of the graphene oxide and has a good targeting property; besides, the modified graphene oxide has good biocompatibility and benefits the biological application of the material; and moreover, the obtained functionalized nanometer material is expected to be used as a drug transmission carrier.

Description

technical field [0001] The invention relates to a graphene oxide / hyaluronic acid nano drug carrier material and a preparation method thereof, belonging to the field of biological application of nano materials. Background technique [0002] Graphene is a new carbon material in which carbon atoms are tightly packed into a single-layer two-dimensional honeycomb lattice structure. Its thickness is only 0.335nm. The basic unit of nanotubes, three-dimensional graphite). Since its discovery in 2004, graphene has quickly become the most active research frontier in the field of materials science and condensed matter physics, while its research in the field of biomedicine has only started in the past two years, and there are still relatively few related work reports. [0003] The graphene derivatives that are widely used in the biomedical field are mainly functionalized graphene oxide. Graphene oxide is the oxide of graphene, which is usually prepared by chemical oxidation and ultra...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K47/04
Inventor 吴惠霞王亚培曾波杨仕平
Owner SHANGHAI NORMAL UNIVERSITY
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