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Application of gingko endogeny Chaetomium globosum in prevention and control of plant pathogenic fungi

A technology for Chaetomium endophytes, phytopathogenic fungi, applied in microorganism-based methods, chemicals for biological control, applications, etc.

Inactive Publication Date: 2012-10-24
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of endophytic fungus---Chaetomium globosum (Chaetomium globosum) NO.05 that is separated and screened out from Ginkgo biloba for the shortage of unsafe chemical pesticides and less types of microbial source pesticides; the present invention Another purpose is to provide the application of using the bacterial strain and its metabolites to antagonize maize leaf rot, grape white rot, and rhizopus stoloniferus

Method used

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  • Application of gingko endogeny Chaetomium globosum in prevention and control of plant pathogenic fungi

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: Isolation and screening of Chaetomium globosa NO.05

[0021] The present invention collected Ginkgo biloba leaves in Linyi, Shandong Province in June 2008, and obtained Chaetomium globosa NO .05, save.

[0022] Determination of the antibacterial activity of Chaetomium globosa No.05: inoculate a 9-cm-diameter piece of corn leaf rot fungus, grape white rot fungus, and rhizopus stoloniferum on the central side of the PDA plate, and spot the chaetomium at a distance of 4.5 cm from the fungus block Bacteria No.05, cultivated at a constant temperature of 28°C for 2-4 days, observe whether there is an antibacterial zone, and measure the size, set the treatment of No. powerful.

[0023] The inhibition rate was calculated by the following formula:

[0024] Inhibition rate (%)=(control group pathogenic bacteria growth radius-treatment group pathogenic bacteria growth radius) / (control group pathogenic bacteria growth radius-original bacteria cake radius) x 100%

...

Embodiment 2

[0026] Embodiment 2: the preparation method of chaetomium globosa NO.05 fermentation metabolite

[0027] Include the following steps:

[0028] (1) Activation culture of strains: move the isolated and preserved Chaetomium globosa strain No.05 onto a potato dextrose PDA solid plate, and cultivate it at 28°C±1°C until the hyphae cover the entire petri dish. A diameter of 9cm is punched to obtain a fungus cake for inoculation;

[0029](2) Fermentation culture: The fermentation culture medium is distilled water per 1000mL, oat 30g, maltose 20g, and pH is 6.0-7.0. Each 500mL triangular bottle contains 200mL of fermentation broth; after preparation, sterilize at 121°C for 20 minutes, cool to 40°C and pick out a bacterium cake with a diameter of 9cm under aseptic conditions. Fermentation culture 7-8d;

[0030] (3) Extraction of fermented product: After the fermentation is completed, the fermented liquid is collected by centrifugation, added with an equal volume of ethyl acetate for...

Embodiment 3

[0031] Example 3: Inhibition of Chaetomium globosa NO.05 fermentation metabolites on maize leaf spot fungus, grape white rot fungus, and rhizopus stoloniferus

[0032] The fermentation metabolite of Chaetomium globosa NO.05 is the fermentation broth prepared in Example 2.

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Abstract

The invention relates to an application of a strain of gingko endogeny Chaetomium globosum NO.05 in prevention and control of plant pathogenic fungi, and belongs to the technical field of biological prevention and control of plant diseases. A strain of the gingko endogeny Chaetomium globosum NO.04 and fermentation metabolites thereof in the present invention can be applied for prevention and control of northern corn leaf blight, Grapevine white rot, and tomato rhizopus fruit rot caused by plant pathogenic fungi. According to the present invention, a microbe strain is provided for prevention and control of northern corn leaf blight, Grapevine white rot, tomato rhizopus fruit rot, and other plant pathogenic fungi diseases on agriculture; and the provided fermentation metabolites can be applied in prevention and control of plant fungi diseases, and a new approach is provided for development of agricultural fungicides.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to the use of an endophytic Chaetomium ginkgo no. stolonifer). Background technique [0002] The drug resistance of plant pathogens is a serious problem in the chemical control of plant diseases, which brings great difficulties to the control of diseases of various plants (such as agricultural crops, economic crops and horticultural crops, etc.). Due to the long-term and extensive use of chemical fungicides, especially systemic fungicides, the resistance of plant pathogens to them is very prominent. It is necessary to overcome the problem of drug resistance through integrated control, especially in combination with biological control, so as to effectively control plant pathogens. [0003] However, most of the current biological control bacteria are still in the initial stage of research and development, far from meeting the actual requireme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C12P1/02C12R1/645
Inventor 潘洪玉张桂珍秦建春刘金亮张雅梅莫华文田野张艳华张婧赢
Owner JILIN UNIV
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