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Bacillus amyloliquefaciens and application thereof

A technology of amylolytic bacillus and bacillus, applied in the field of agricultural microorganisms, can solve problems such as low soil nutrients, low crop yield, and saline-alkali hazards, and achieve the effects of increasing soil available potassium content, promoting absorption, and improving utilization rate

Active Publication Date: 2012-10-24
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Huang-Huai-Hai Plain with the largest distribution area of ​​fluvo-aquic soil suffers from frequent droughts and floods, and there are still saline-alkali hazards. In addition, the soil nutrients are low or lacking.

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] First prepare the following three media.

[0042] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C for 20min.

[0043] LB liquid medium: without agar, other conditions are the same as above.

[0044] Liquid potassium-dissolving bacterial culture medium: 10.0g sucrose, 0.5g yeast extract, (NH 4 ) 2 SO 4 1.0g, Na 2 HPO 4 2.0g, MgSO 4 ·7H 2 O0.5g, CaCO 3 1.0g, 1.0g potassium feldspar powder, 1000mL distilled water, sterilized at 121°C for 20min.

[0045] Nitrogen fixation capacity using Ashby nitrogen-free medium: mannitol 10g, KH 2 PO 4 0.2g, MgSO 4 0.2g, NaCl 0.2g, K 2 SO 4 0.3g, CaCO 3 5g, distilled water 1000mL, Agar 15g, sterilized at 121°C for 20min.

[0046]Inorganic salt medium: ammonium sulfate 2.0g; sodium dihydrogen phosphate 0.5g; dipotassium hydrogen phosphate 0.5g; magnesium sulfate heptahydrate 0.2g; calcium chloride dihydrate 0.1g, distilled water 1000mL, pH7...

Embodiment 2

[0056] Aerobic test

[0057] Pour the sterilized LB medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, use the inoculation needle to pick up the strain JX1 (CGMCC No.5624) cultured on the slant, Puncture into the above medium (must pierce to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria. The test results showed that the colonies of the strain JX1 (CGMCC No.5624) grew along the surface of the agar column, and there were also colonies in the puncture line, which was facultatively anaerobic.

[0058] Determination of catalase

[0059] Put 1 drop of 3% HO on a clean slide 2 o 2 , take 1 ring of LB slant culture of strain JX1 (CGMCC No.5624) cultivated for 18~24h, and in H 2 o 2 Smear in the middle, if bubbles are produced, it ...

Embodiment 3

[0085]In order to further verify the indole acetic acid production ability and optimal conditions of the rhizosphere growth-promoting bacteria JX1 (CGMCC No.5624) obtained in Example 1, the following explores the indole acetic acid for different pH, liquid volume, different carbon sources, and different nitrogen sources. Influence of indoleacetic acid production.

[0086] Put 25ml, 50ml, 75ml, 100ml, 150ml of LB liquid medium containing L-tryptophan (100mg / L) into a 250ml Erlenmeyer flask, and inoculate it in the logarithmic growth phase at 1% (v / v) inoculum After JX1 (CGMCC No.5624), put it at 30℃, 180r.min -1 Cultivate on a shaking table for 24 hours, and measure the amount of IAA produced by a quantitative method. The result is as figure 2 As shown, because the strain JX1 (CGMCC No.5624) is a facultative anaerobic metabolism, the aeration rate affects the efficiency of the strain to produce IAA. When the liquid volume is 150mL, the strain produces the most IAA, and then ...

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Abstract

The invention belongs to the field of agriculture microbe and discloses bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens. The rhizosphere growth promoting rhinoacteria JX1 is named as bacillus amyloliquefaciens (bacillus amyloliquefaciens) in class, and conserved in the common microbe center of China microbial culture collection management committee on Dec 20, 2011, and the conservation number is CGMCC No.5624. The rhizosphere growth-promoting rhinoacteria JX1 (CGMCC No.5624) can produce heteroauxin with high yield and grow by utilizing insoluble metasilicate containing potassium as a potassium source. Therefore, the bacillus amyloliquefaciens JX1 can be applied to promoting plant growth.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a bacillus amyloliquefaciens and its application. Background technique [0002] Fluvo-aquic soil is the soil formed by river sediments affected by groundwater movement and farming activities. It is named after the night tide phenomenon. In China, it is mostly distributed in the alluvial plains of the middle and lower reaches of the Yellow River, the plains of Jiangsu and Anhui to the south, and the river, lake plains and deltas of the middle and lower reaches of the Yangtze River Basin. The distribution area of ​​fluvo-aquic soil is flat, with deep soil layer, abundant water and heat resources, and wide variety of seed production. It is the main dryland soil in my country and is rich in grain and cotton. However, the Huang-Huai-Hai Plain, where the fluvo-aquic soil is the largest, suffers from frequent droughts and floods, as well as saline-alkali hazards. In addition, s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C05F11/08A01G1/00A01G7/00C12R1/07
Inventor 李辉信姜瑛徐文思吴越虞丽胡锋焦加国徐莉刘满强陈小云
Owner NANJING AGRICULTURAL UNIVERSITY
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