Bacillus amyloliquefaciens and application thereof
A technology of amylolytic bacillus and bacillus, applied in the field of agricultural microorganisms, can solve problems such as low soil nutrients, low crop yield, and saline-alkali hazards, and achieve the effects of increasing soil available potassium content, promoting absorption, and improving utilization rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0040] Example 1
[0041] First prepare the following three media.
[0042] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C, 20min.
[0043] LB liquid medium: without agar, other conditions are the same as above.
[0044] Liquid potassium-dissolving bacterial culture medium: sucrose 10.0g, yeast extract 0.5g, (NH 4 ) 2 SO 4 1.0g, Na 2 HPO 4 2.0g, MgSO 4 ·7H 2 O0.5g, CaCO 3 1.0g, 1.0g potassium feldspar powder, 1000mL distilled water, sterilized at 121°C, 20min.
[0045] Nitrogen fixation capacity adopts Ashby nitrogen-free medium: mannitol 10g, KH 2 PO 4 0.2g, MgSO 4 0.2g, NaCl 0.2g, K 2 SO 4 0.3g, CaCO 3 5g, distilled water 1000mL, Agar 15g, 121℃ sterilized, 20min.
[0046] Inorganic salt culture medium: ammonium sulfate 2.0g; sodium dihydrogen phosphate 0.5g; dipotassium hydrogen phosphate 0.5g; magnesium sulfate heptahydrate 0.2g; calcium chloride dihydrate 0.1g, distilled water 1000mL, pH7.0, 121℃ s...
Example Embodiment
[0055] Example 2
[0056] Aerobic test
[0057] Pour the sterilized LB medium into 3 sterilized test tubes, about 2 / 3, on the aseptic operation table, use the inoculating needle to pick the slant cultured strain JX1 (CGMCC No.5624), The puncture is inoculated into the above medium (must puncture to the bottom of the tube). Incubate at 30°C and observe the results in 3 to 7 days. Those growing on the surface of the agar column are aerobic bacteria, such as those growing along the puncture line are anaerobes or facultative anaerobes. The test results showed that the colonies of strain JX1 (CGMCC No. 5624) grew along the surface of the agar column, and colonies also grew in the puncture line, which was facultative anaerobic.
[0058] Determination of catalase
[0059] Put 1 drop of 3% H on a clean glass slide 2 O 2 , Take 1 loop of LB slant culture of strain JX1 (CGMCC No. 5624) cultivated for 18~24h and place it in H 2 O 2 Medium smear, if there are bubbles, it is positive, otherwise...
Example Embodiment
[0084] Example 3
[0085] In order to further verify the ability of the rhizosphere growth-promoting bacteria JX1 (CGMCC No. 5624) obtained in Example 1 to produce indole acetic acid and the optimal conditions, the following is to explore the indole acetic acid for different pH, liquid volume, different carbon sources, and different nitrogen sources. The effect of indole acetic acid production.
[0086] Put 25ml, 50ml, 75ml, 100ml, 150ml of LB liquid medium containing L-tryptophan (100mg / L) in a 250ml Erlenmeyer flask, and inoculate 1% (v / v) inoculum in logarithmic growth phase After the JX1 (CGMCC No.5624), put it at 30℃, 180r.min -1 Incubate on a shaker for 24 hours, and determine the amount of IAA produced by quantitative determination. The result is figure 2 As shown, because the strain JX1 (CGMCC No. 5624) is facultative anaerobic metabolism, the ventilation affects the efficiency of the strain to produce IAA. When the liquid volume is 150mL, the strain produces the most IAA...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap