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Method for extracting and separating L-leucine by using combined technology of membrane separation and electrodialysis

A technology of leucine and fermentation broth, applied in the field of extraction and separation of L-leucine, can solve the problems of affecting product quality and yield, incomplete removal of bacterial cells, broken resin structure, etc., and achieves ionic impurities and The effect of reducing the pigment content, reducing the decolorization process of activated carbon, and improving the light transmittance of the material liquid

Inactive Publication Date: 2012-10-31
WUXI JINGHAI AMINO ACID
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The general process of this process is: filter the fermentation broth to remove bacteria, and ion exchange to separate amino acids through the process of adsorption and elution. There are the following problems in the separation and extraction of L-leucine from the fermentation broth by using this process: one is to use the method of filtration to remove bacteria Because the bacteria are small, usually the bacteria are not completely removed; the second is that the color value of the L-leucine fermentation liquid is relatively high, the decolorization of ordinary activated carbon is not complete and the amount is large, which affects the product quality and yield; the third is L-leucine There are many impurities and high content in the acid fermentation liquid, and the direct ion exchange method will cause serious resin pollution, which will easily cause the resin structure to be broken and lose its regeneration ability, which will increase the operating cost. At the same time, the high content of ionic substances will quickly saturate the resin , increase the number of resin regeneration, resulting in a large amount of sewage discharge and other problems
Membrane separation technology has been used in the separation and purification of fermentation products. There are successful precedents, but the use of membrane separation and electrodialysis combined technology to separate and extract L-leucine from fermentation broth has not been reported.

Method used

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  • Method for extracting and separating L-leucine by using combined technology of membrane separation and electrodialysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 5.0L of L-leucine fermentation broth was filtered through an ultrafiltration membrane with a molecular weight cut-off of 200,000 to remove bacteria. The operating pressure was 0.3MPa, the temperature was 30°C, and the volume of dialysis water was 1.5 L. Then through a 6L combined electrodialysis membrane device at a temperature of 30°C, a desalinated L-leucine supernatant was obtained. The desalinated clear liquid is filtered through a nanofiltration membrane with a molecular weight cut-off of 500 to remove small molecular impurities and pigments. The operating pressure is 1.2MPa, the temperature is 30°C, and the dialyzed water volume is 3.6L, and then concentrated and crystallized by conventional methods to obtain the product. The results are shown in Table 1 below:

[0029] Electrodialysis electric drive membrane separator: stock solution conductivity: 22400μS / cm

[0030] Conductivity of desalted liquid: 500μS / cm

[0031] ...

Embodiment 2

[0035] 350L of L-leucine fermentation broth was filtered through an ultrafiltration membrane with a molecular weight cut-off of 800,000 to remove bacteria. The operating pressure was 0.36 MPa, the temperature was 30°C, and the volume of dialysis water was 120L. Then go through a 500L combined electrodialysis membrane device at a temperature of 30°C to obtain a desalinated L-leucine serum. The desalted clear liquid is filtered through a nanofiltration membrane with a molecular weight cut-off of 500 to remove small molecular impurities and pigments. The operating pressure is 1.2MPa, the temperature is 30°C, and the dialyzed water volume is 240L. Then, the product is concentrated and crystallized by conventional methods. The results are shown in Table 2 below:

[0036] Electrodialysis electric drive membrane separator: stock solution conductivity: 22500μS / cm

[0037] Conductivity of desalted liquid: 600μS / cm

[0038] Salt re...

Embodiment 3

[0042] L-leucine fermentation broth 4.5M 3 The bacterial cells are removed by ultrafiltration with a molecular weight cut-off of 800,000, the operating pressure is 0.4MPa, the temperature is 30°C, and the dialyzed water volume is 1.5 M 3 . The filtrate completely removes the bacteria, and then passes through 8M 3 The combined electrodialysis membrane equipment is used at a temperature of 30°C to obtain desalinated L-leucine supernatant. The desalted clear liquid is filtered through a nanofiltration membrane with a molecular weight cut-off of 1000 to remove small molecular impurities and pigments. The operating pressure is 1.2MPa, the temperature is 30°C, and the dialyzed water volume is 3.1M. 3 , and then use conventional methods to concentrate and crystallize the product. The results are shown in Table 3 below:

[0043] Electrodialysis electric drive membrane separator: stock solution conductivity: 22650μS / cm

[0044] Conductivity of desalted liquid: 600μS / cm

[0045] S...

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Abstract

The invention discloses a method for extracting and separating L-leucine by using a combined technology of membrane separation and electrodialysis, and belongs to the technical field of biochemical engineering. The invention comprises the following steps: (1) removing thallus from fermentation liquid; (2) removing inorganic salt through electrodialysis; (3) removing inorganic salt clear liquid and small-molecule impurities; and (4) concentrating and crystallizing the fermentation liquid. The method has the advantages that the condition is mild, the operation is simple and convenient, the separation steps are less, the selectivity is good, and the production is clean, overcomes the defects of low yield, large sewage discharge amount and high production strength in the conventional ion exchange technology, and improves the yield and the quality of L-leucine remarkably.

Description

technical field [0001] The invention relates to a method for extracting and separating L-leucine, in particular to a method for separating and extracting inorganic salts from L-leucine fermentation liquid by using membrane separation and electrodialysis combined technology, belonging to the technical field of biochemical industry . Background technique [0002] As one of the branched-chain amino acids, L-leucine is an essential amino acid for the human body and has various physiological functions. Because of its special structure and function, it occupies an important position in the metabolism of human life. It is mainly used to prepare compound amino acid infusions, synthetic polypeptide drugs and food antioxidants, etc., especially in medical research and treatment, and is increasingly valued. It is used in the treatment of blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, dietary treatment of congenital metabolic defects, treatment of sepsis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/08C07C227/40
Inventor 宁健飞蔡立明
Owner WUXI JINGHAI AMINO ACID
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