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Preparation method for energy-saving biological straw feed

A biological straw and grain-saving technology, applied in animal feed, animal feed, application, etc., can solve the problems of low degradation rate of cellulose and hemicellulose, complex process, low number of viable probiotic bacteria, etc., and achieve the goal of cellulose High enzyme activity, simple production process, and the effect of a large number of beneficial viable bacteria

Active Publication Date: 2014-07-02
JIAXING YILING BIOLOGICAL TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of sufficient understanding of straw structure and efficient microbial breeding, the straw feed produced by this technology is limited to ruminants and difficult to promote production. The main problems are as follows:
[0006] (1) Straw lacks pretreatment before fermentation or only adopts simple physical treatment such as forging or crushing, which cannot effectively break the straw structure, and the degradation rate of cellulose and hemicellulose is low
Patent CN03105080 uses multi-strain combined anaerobic fermentation to produce straw protein feed. Straw is forged into 3-5cm or crushed into 20 meshes. The crushing particle size is difficult to break the straw structure and can not greatly improve the nutritional content of straw
[0007] (2) The number or types of microorganisms used in fermentation are too many, resulting in cumbersome production process, long fermentation cycle, difficult product quality control and low production efficiency
Patent CN98110370 discloses a straw feed fermentation process, the process involves lactic acid bacteria, yeast, biomimetic flora (Aspergillus niger, Aspergillus oryzae, Trichoderma and Candida) and other fermenting microorganisms, fermented at 30-40°C for 10-15 Days to obtain fermented feed, complex process
[0008] (3) Microorganisms used in fermentation do not have the ability to degrade cellulose and hemicellulose, and cannot effectively improve the metabolic energy of straw, and the utilization rate of straw is low
Patent CN200810016743 Soak the straw crushed to 2-3cm in the yeast culture solution for 9 hours to ferment, but the yeast does not have the enzyme system to degrade cellulose and hemicellulose
[0009] (4) The safety of microorganisms used in fermentation is uncertain, and may be harmful bacteria or molds
Trichoderma reesei involved in the feed starter disclosed in patent CN200510066003 and patent CN201010561969 does not belong to the feed additive catalog of the Ministry of Agriculture, and its safety cannot be determined
[0010] (5) The lactic acid content of the prepared straw feed is low, the number of viable probiotic bacteria is low, the palatability is poor, the feeding effect is not good, and the grain saving effect is not significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 45 kg of corn stalks, 11 kg of bran, 8 kg of corn, 8 kg of secondary powder, 12 kg of soybean meal and 16 kg of rapeseed meal to form a dry material, wherein the above-mentioned corn stalks are pulverized to a particle size of 300 mesh through a multistage cyclone mill; : The weight ratio of 0.5 is to fully mix the above-mentioned dry material with water and stir evenly, and carry out high-pressure sterilization at 100°C for 10 minutes; inoculate 15% of the activated Pediococcus pentosaceae and 5% of the activated black mold of the raw material weight after sterilization , add 0.5 FPU / g cellulase at the same time, after stirring evenly, put it into a microbial breathing bag with a one-way exhaust valve, carry out solid-state fermentation at 28°C, and the fermentation time is 72h; after the fermentation is completed, the finished product of straw feed is obtained. The feed has an obvious sour flavor, and the lactic acid content of the feed reaches 1.3% of the total ...

Embodiment 2

[0033] Weigh 40kg of corn stalks, 10kg of bran, 12kg of corn, 10kg of secondary flour, 10kg of soybean meal and 18kg of rapeseed meal to form a dry material. : The weight ratio of 0.5 is to fully mix the above-mentioned dry material with water and stir evenly, and carry out high-pressure sterilization at 112°C for 10 minutes; inoculate into 30% activated Pediococcus pentosaceae and 10% activated Bacillus subtilis by weight of raw materials after sterilization Bacillus, add 2FPU / g cellulase at the same time, after stirring evenly, put it into a microbial breathing bag with a one-way exhaust valve, and carry out solid-state fermentation at 35°C for 48 hours; after the fermentation is completed, the finished product of straw feed is obtained. The feed has an obvious sour flavor, the lactic acid content of the feed reaches 2.2% of the total weight of the feed, and the number of beneficial viable bacteria is 4×10. 9 CFU / g, cellulase activity is 2.2FPU / g, and the degradation rate of...

Embodiment 3

[0035] Weigh 35kg of corn stalks, 13kg of bran, 12kg of corn, 10kg of secondary powder, 12kg of soybean meal and 18kg of rapeseed meal to form a dry material. : weight ratio of 1, mix the above dry material with water and stir evenly, then carry out autoclave sterilization at 112°C for 10 minutes; inoculate into 20% activated Pediococcus pentosaceae and 5% activated Aspergillus oryzae by weight of the raw material after sterilization , while adding 1.5 FPU / g cellulase, after stirring evenly, put it into a microbial breathing bag with a one-way exhaust valve, and carry out solid-state fermentation at 40°C for 48 hours; after the fermentation is completed, the finished product of straw feed is obtained. The feed has an obvious sour flavor, the lactic acid content of the feed reaches 2.1% of the total weight of the feed, and the number of beneficial viable bacteria is 3×10 9 CFU / g, cellulase activity is 2.0FPU / g, and the degradation rate of cellulose and hemicellulose is 25%.

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PUM

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Abstract

The invention discloses a preparation method for an energy-saving biological straw feed. The preparation method comprises the following steps: combining 35-45 percent of maize straw, 8-13 percent of bran, 8-12 percent of maize, 8-10 percent of wheat shorts, 10-12 percent of soybean meal and 16-18 percent of rapeseed dregs to form dry materials; mixing the dry materials with water and uniformly stirring; sterilizing the mixture at high pressure and inoculating the mixture to a microorganism according to the inoculum size which accounts for 10-40 percent of the weight of raw materials; meanwhile, adding 0.5-2 FPU (Filter Paper Unit) / g cellulase; and carrying out solid-stage fermentation at the temperature of 28-45DEG C for 36-72 hours to obtain a finished straw feed, wherein the maize straw is subjected to multistage cyclone grinding and crushing until the granularity is 200-300 meshes and the microorganism is preferably a mixture of bacillus subtilis and pediococcus pentosaceus. The preparation method disclosed by the invention is simple in process and is short in fermentation period; the maize straw prepared by the preparation method can replace maize components with high proportion in pig feed; and the prepared feed has the advantages of remarkable acid fragrance, favorable palatability, high lactic acid content, more beneficial variable counts, high activity of cellulase, high degradation rate of cellulose and hemicellulose and favorable safety.

Description

technical field [0001] The invention belongs to the technical field of animal feed, and in particular relates to a method for preparing straw feed by using corn straw as a main raw material and utilizing microbial fermentation. Background technique [0002] my country is a large agricultural country, and crop straw is an important biological resource because of its large output, wide distribution and variety. According to statistics, in 2010, the total output of straw in my country was about 700 million tons. However, these straw resources have not been rationally developed and utilized for a long time. With the transformation of agricultural production methods and the improvement of rural living conditions, the phenomenon of random discarding and burning of straw is serious, which not only wastes resources but also pollutes the environment. Therefore, accelerating the comprehensive utilization of straw is conducive to alleviating resource constraints and reducing environm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14
CPCY02P60/87
Inventor 陆健吴亮王璐璐王程朱德伟波利托夫蔡国林拉巴乔夫
Owner JIAXING YILING BIOLOGICAL TECH
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