Unlock instant, AI-driven research and patent intelligence for your innovation.

Deoxynivalenol toxin degrading enzyme as well as encoding gene and application thereof

A technology of deoxynivalenol and coding gene, applied in the field of enzyme engineering, can solve the problems of non-allowance, loss of nutrients, change of food quality, etc., and achieve the effect of controlling food pollution

Active Publication Date: 2012-12-12
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of physical and chemical detoxification is not as high as expected, and it changes the quality of food and easily causes the loss of nutrients. The EU does not allow the application of chemical methods to eliminate mycotoxins in the food production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Deoxynivalenol toxin degrading enzyme as well as encoding gene and application thereof
  • Deoxynivalenol toxin degrading enzyme as well as encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Acquisition of deoxynivalenol degrading enzyme gene Dasag and expression of deoxynivalenol degrading enzyme

[0028] Construction of Plasmid Library of Deoxynivalenol Degrading Enolase Gene

[0029] (1) After activating the standard strain Fusarium oxysporum ACCC No.36245 (purchased from China Agricultural Microorganism Culture Collection and Management Center), it was inserted into PDA liquid medium (potato 200g / L, glucose 20g / L, agar 20g / L, The pH is natural), and then the suspension of the strain was added to 100ml of the same medium, and the shaker condition was 220 rpm, 30°C, 72 hours. After the cultivation was completed, the bacteria were collected by centrifugation at 12000 rpm. RNA extraction (Trizol method);

[0030] (2) Using RNA as a template to reverse transcribe and synthesize a cDNA sequence;

[0031] 2. Acquisition of deoxynivalenol degrading enzyme gene

[0032] Using the cDNA sequence obtained in step 1 as a template, a PCR reaction was pe...

Embodiment 2

[0042] Example 2 Activity detection of deoxynivalenol degrading enzyme

[0043] Sample processing: Recombinant Pichia pastoris ASAG2 strain (experimental group) and wild type Pichia pastoris GS115 strain (control group, empty plasmid integration strain) induced expression. After 5 days, 800 μL of the supernatant was taken and placed in a 1.5 mL centrifuge tube, and then 200 μL of 100 ppm DON was added to make the final concentration reach 20 ppm. After the addition, react at 30°C for 0h-5h for post-treatment. Take 10 μL of the processed sample and enter the high performance liquid chromatography to detect the residue of DON. The detection method is carried out according to the GB / T 23504-2009 method. HPLC detection conditions: Agilent Eclipse XDB-C18, 150mm×4.6mm (5μm) chromatographic column, mobile phase: water / methanol=80% / 20%, UV detector detection, column temperature 30°C, flow rate 1.0ml / min, the injection volume is 10 μL. Through the detection of high performance li...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a deoxynivalenol (DON) toxin degrading enzyme as well as an encoding gene and application thereof. The enzyme derives from fusarium oxysporum, which is the following protein (a) or (b): (a) a protein consisting of amino acid residue sequences of SEQ ID NO:1 in a sequence table; or (b) a protein which has activity for degrading deoxynivalenol, is derived from SEQ ID NO:1, and is obtained by subjecting the amino acid residue sequence SEQ ID NO:1 in the sequence table to substitution and / or deletion and / or addition of one or more amino acid residues. The deoxynivalenol toxin degrading enzyme has efficient degrading effect on DON.

Description

technical field [0001] The invention belongs to the field of enzyme engineering, and in particular relates to a deoxynivalenol degrading enzyme and its coding gene, and the application of the deoxynivalenol degrading enzyme. Background technique [0002] Deoxynivalenol (Deoxynivalenol, DON) is a secondary metabolite produced by Fusarium when it lacks nutrients, mainly from Fusarium graminearum and Fusarium yellow, and Fusarium graminearum is the cause of wheat head blight and an important pathogen of corn ear rot. DON is a global cereal pollutant with the highest level of fusarium toxins, which mainly occurs in wheat, barley, oats, rye and corn, but rarely occurs in rice, sorghum and triticale. The pollution of deoxynivalenol has not only caused great harm to human and animal health, resulted in huge economic losses, but also led to a large number of international trade disputes. [0003] At present, the detoxification methods of DON at home and abroad mainly include physi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12N15/52C12N15/63C12N5/10C12N1/21C12N1/19A62D3/02C12R1/77C12R1/84A62D101/28
Inventor 吴子丹伍松陵孙长坡任保中
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION