Test strip for identifying foot-and-mouth-disease virus infected and vaccine immunized animal at one step and preparation method of test strip

A technology for immunizing animals with foot-and-mouth disease virus, which is applied in the field of test strips for rapid identification of foot-and-mouth disease virus infection and vaccine immunization animals and the preparation thereof, can solve the problems of poor sensitivity, laboriousness, complicated operation and the like, and achieves high specificity, sensitivity, The results show the effect of intuitive, strong detection specificity

Active Publication Date: 2012-12-12
HENAN ACAD OF AGRI SCI
View PDF4 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technique is accurate and reliable, but the sensitivity is poor, and there is complement activity in some samples, which affects the detection effect, and requires a specialized laboratory
(2) Virus neutralization test: The interaction between the virus and specific serum neutralizing antibodies is used to make the virus lose its ability to infect susceptible animals and sensitive cells. This method must use live viruses, which cannot be operated by ordinary laboratories
The ELISA method has the advantages of specificity and sensitivity in determination, but the operation is complex, time-consumi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Test strip for identifying foot-and-mouth-disease virus infected and vaccine immunized animal at one step and preparation method of test strip
  • Test strip for identifying foot-and-mouth-disease virus infected and vaccine immunized animal at one step and preparation method of test strip
  • Test strip for identifying foot-and-mouth-disease virus infected and vaccine immunized animal at one step and preparation method of test strip

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0051] 3. Preparation of Gold Standard SPA glass wool:

[0052] Preparation of gold sol by sodium citrate reduction method: add 2 to 4 ml of 0.5 to 2% trisodium citrate solution to 50 to 100 ml of boiling 0.01 to 0.05% chloroauric acid aqueous solution to obtain colloidal gold with a diameter of about 15 nanometers ; with K of 0.1mol / L 2 CO 3 Adjust the pH of the colloidal gold to 8.5-9.5, add the SPA to be labeled into the gold sol with a pH of 8.5-9.5 at a labeling ratio of 1:1000-1300, add 20% PEG10000 to the final concentration of 0.05% after labeling for 10 minutes , centrifuge at 4°C, 1500-3000 rpm for 20 minutes, remove unbound colloidal gold particles, centrifuge at 4°C, 15,000 rpm for 1 hour, discard the supernatant, and obtain colloidal gold-labeled SPA; 500 diluted colloidal gold-labeled SPA was adsorbed in refined glass wool, and dried under vacuum at 4°C to prepare gold-labeled SPA glass wool.

[0053] 4. The detection principle of the test strip of the present...

Embodiment 1

[0060] Embodiment one: one step distinguishes the test strip of foot-and-mouth disease virus infection and vaccine immune animal, see figure 1 , figure 2 In the figure, the support layer 1 is made of hard plastic thin strips, the sample adsorption fiber layer 2 is made of glass wool, the gold-labeled antibody fiber layer 3 is made of glass wool with colloidal gold-labeled SPA adsorbed, and the cellulose film layer 4 It is made of nitrocellulose membrane, and the water-absorbing material layer 5 is made of water-absorbing filter paper; the sample absorbing fiber layer 2, the gold-labeled antibody fiber layer 3, the cellulose film layer 4, and the water-absorbing material layer 5 are arranged from the left end (test end) to the right Paste on the hard plastic thin strips of the support layer 1 in turn, and the fibers at the junction between each other cross-penetrate each other; on the cellulose film layer 4, there are porcine foot-and-mouth disease virus structural protein VP...

Embodiment 2

[0062] Embodiment two: the test strip structure is basically the same as embodiment one, the difference is:

[0063] The gold-labeled antibody fiber layer 3 is made of glass wool adsorbed with colloidal gold-labeled SPA, and the detection blot 6-P printed with the porcine foot-and-mouth disease virus structural protein VP1 antigen solution expressed and purified by the Escherichia coli expression system is used for sheep (rabbit) The control blot C printed by the anti-SPA IgG solution, the combined blot band 7 formed by the arrangement of the two blots is "｜｜” .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a test strip for rapidly identifying a foot-and-mouth-disease virus infected and vaccine immunized animal and a preparation method of the test strip. The test strip comprises a supporting layer and an adsorbing layer; the adsorbing layer is attached to the supporting layer; the adsorbing layer comprises a sample adsorbing fiber layer, a gold-labeled antibody fiber layer and a cellulose membrane layer sequentially arranged on the testing end and further comprises a water absorbing material layer arranged on the handle end; a detection blot and a control blot are arranged on the cellulose membrane layer; the colloidal gold labeled staphylococcus protein A is adsorbed on the gold-labeled antibody fiber layer; the detection blot is printed by one or two of a pig foot-and-mouth-disease virus protein structural VPI antigen and a non-structural protein 3ABC antigen which are expressed and purified by an escherichia coli expression system; and the control blot is printed by using a goat anti- or rabbit anti-SPA IgG antibody solution. The test strip is high in specificity, high in sensitivity, simple, direct, exact, wide in application scope, low in cost, capable of being used by professionals or non-professionals for detection at any time and any place and beneficial to popularization and application.

Description

technical field [0001] The invention relates to a device for detecting foot-and-mouth disease virus, in particular to a test strip for quickly identifying foot-and-mouth disease virus infection and vaccine-immunized animals and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is a severe infectious disease of cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV). FMDV has the characteristics of polymorphism, variability, wide range of hosts, and strong infectivity. Once it occurs, it will become epidemic and large-scale outbreaks. The outbreak and prevalence of FMD often cause huge losses to the affected areas. In addition to the direct economic losses caused by animal deaths, there are also stagnation of meat and milk production during animal illness, long-term decline in meat and milk production after recovery, and seed value. Loss and other major losses. What's more serious is that FMD is a severe infectious disease,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/569G01N33/558
Inventor 张改平杨苏珍卢清侠职爱民杨继飞乔松林邓瑞广
Owner HENAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap