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Method for preparing Fructus Aurantii ingredients having anti-liquid-accumulation activity, and use

A technology of citrus aurantium and column volume, which is applied in the application field of preparing non-alcoholic fatty liver treatment drugs, can solve the problems of unclear components of citrus aurantium, achieve simple and clear chemical components, simple extraction and separation process, and good pharmacological activity Effect

Active Publication Date: 2012-12-19
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the components of Fructus Fructus Aurantii have the activity of inhibiting lipid accumulation in liver cells is not clear

Method used

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  • Method for preparing Fructus Aurantii ingredients having anti-liquid-accumulation activity, and use
  • Method for preparing Fructus Aurantii ingredients having anti-liquid-accumulation activity, and use
  • Method for preparing Fructus Aurantii ingredients having anti-liquid-accumulation activity, and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of effective components of Fructus Fructus Fructus Aurantii

[0032] Add 4200ml 70% ethanol to 1500g Citrus aurantii and extract for 2 hours, add 3500ml 70% ethanol to extract for 1.5 hours, and finally add 2800ml 70% ethanol to extract for 1 hour, combine three extracts, filter and concentrate. The concentrated solution was passed through macroporous resin (D101), firstly eluted with water, then with 20% ethanol, and finally with 70% ethanol. Collect 70% ethanol eluate, concentrate to about 150ml, suction filter, pass through polyamide column, elute with 20% ethanol for 4 column volumes, collect the eluate; then elute with 40% ethanol for 5 column volumes, divide Collect the eluate in 10 sections, 500ml in each section. The elution section of 20% ethanol was rotary evaporated, freeze-dried and weighed to obtain 47.5608g of Fructus Aurantii fraction. It was used in the biological tests below.

Embodiment 2

[0033] Embodiment two The analysis of the effective components of Fructus Aurantii

[0034] Chromatographic conditions Agilent 1100 high performance liquid chromatography, the chromatographic column is SB-C 18 Chromatographic column; use acetonitrile-water (20:80) (adjust the pH value to 3 with phosphoric acid) as the mobile phase; the detection wavelength is 283nm; the liquid chromatography flow rate is 1ml / min, and the injection volume is 10μl.

[0035] Preparation of the test solution Weigh this product, dissolve it in a volumetric flask with methanol solution, dilute to 2mg / ml, shake well, and get it.

[0036] test methods Precisely absorb the test solution, inject it into a liquid chromatograph, and measure it according to the chromatographic analysis conditions.

[0037] analysis results The high-performance liquid chromatography analysis spectrum of the active components of Fructus Fructus Fructus is shown in figure 1 , the chromatographic peaks with ...

Embodiment 3

[0038] Embodiment three active ingredient preparation of Fructus aurantii

[0039] Take 0.5 g of the active components of Fructus Fructus Fructus obtained in Example 1 and mix them evenly with 10.5 g of polyethylene glycol-6000, heat and melt, and after the chemical material is transferred to drip irrigation, the drug solution is dropped into liquid paraffin at 6-8°C. Degreasing, made 400 dropping pills.

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Abstract

The invention relates to a method for preparing Fructus Aurantii ingredients having anti-liquid-accumulation activity and a use, concretely to a Fructus Aurantii extractive containing 20 to 42 % of naringin and 20 to 48 % of neohesperidin. The Fructus Aurantii extractive is prepared by the following method comprising smashing the Fructus Aurantii medicinal materials, adding 20 to 90 % ethanol, extracting for 1 to 3 times under reflux by heating, combining filtrates to get extracting solutions, condensing the extracting solutions to get extractum, mixing the extractum with macroporous resin, purifying with a macroporous resin column, collecting 60 to 80 % ethanol eluates, condensing, purifying with a polyamide column, collecting 20 % ethanol eluates, condensing and drying to get the Fructus Aurantii ingredients. The Fructus Aurantii extractive can be used for preparing medicaments for treating non-alcoholic fatty liver.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and in particular relates to an effective component extracted from Fructus Fructus Fructus Fructus Fructus and its preparation method, as well as the application of the component in the preparation of non-alcoholic fatty liver medicine. Background technique [0002] Liver injury is a complex process caused by multiple etiologies and involving multiple factors. Liver disease caused by liver injury has become a common and frequently-occurring disease. What is more serious is the liver failure and hepatic encephalopathy caused by it. The morbidity and mortality are high, which greatly threatens human health. Many factors can cause liver damage. Nonalcoholic fatty liver disease (NAFLD), also known as nonalcoholic fatty liver (NAFL), is a disease characterized by fatty degeneration and fat accumulation in hepatic parenchymal cells without a history of excessive drinking clinico...

Claims

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Application Information

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IPC IPC(8): A61K36/752A61K31/7048A61P1/16
Inventor 程翼宇王怡王毅刘二伟
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE