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Process for separating and purifying natamycin

A technology for separation and purification of natamycin, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of long process, high loss of organic solvent, high production cost, etc., and achieve the improvement of filtrate quality, The effect of improving product yield and reducing filtration cost

Inactive Publication Date: 2013-01-09
北京明新高科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a process for extracting and purifying natamycin from biological fermentation liquid, which is characterized by extracting natamycin from fermentation liquid by non-organic solvent method, and solves the problem of extracting natamycin by organic solvent method The problem of high production cost caused by high organic solvent loss and long process during the extraction and purification of natamycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A kind of separation and purification method of Natamycin of embodiment 1, carry out successively according to the following steps:

[0016] 1. Take 20L (the content of natamycin is 9.0g / L) fermentation broth, adjust the pH to 3.0 with oxalic acid, add yellow blood salt and zinc sulfate in turn, stir, and process for about 0.5h, then add sodium polyacrylate, temperature control Below 30°C, the total treatment time shall not exceed 3h.

[0017] 2. Filter the treated natamycin fermentation broth under a pressure of 0.05Mpa through a chamber diaphragm filter press to obtain a clear and transparent filtrate for membrane extraction.

[0018] 3. Titanium alloy membrane separation device (with recoil system), membrane filtration pore size 0.1μm, multi-channel tubular membrane filtration area (0.25±0.05)m 2 . Equipped with a liquid storage tank of 40L, a titanium alloy membrane tube of 0.1 μm, a pH value of 6.0, an operating pressure of 0.6 MPa, an operating time of 60 minute...

Embodiment 2

[0021] A kind of separation and purification method of Natamycin of embodiment 2, carry out successively according to the following steps:

[0022] 1. Take 30L of natamycin fermentation broth, adjust the pH to 2.8 with oxalic acid, add yellow blood salt and zinc sulfate in turn, stir, and treat for about 0.5h, then add sodium polyacrylate, control the temperature below 30°C, and complete the treatment The time does not exceed 3h.

[0023] 2. Filter the treated natamycin fermentation broth under a pressure of 0.07Mpa through a chamber diaphragm filter press to obtain a clear and transparent filtrate for membrane extraction.

[0024] 3. Titanium alloy membrane separation device (with recoil system), membrane filtration pore size 0.1μm, multi-channel tubular membrane filtration area (0.25±0.05)m 2 . Equipped with a liquid storage tank of 40L, a titanium alloy membrane tube of 0.1μm, a pH value of 6.5, an operating pressure of 0.8MPa, an operating time of 100min, a membrane surf...

Embodiment 3

[0027] A kind of separation and purification method of natamycin of embodiment 3, carry out successively according to the following steps:

[0028] 1. Take 40L of natamycin fermentation broth, adjust the pH to 2.9 with oxalic acid, add yellow blood salt and zinc sulfate in turn, stir, and treat for about 0.5h, then add sodium polyacrylate, control the temperature below 30°C, and complete the treatment The time does not exceed 3h.

[0029] 2. Filter the treated natamycin fermentation broth under a pressure of 0.06Mpa through a chamber diaphragm filter press to obtain a clear and transparent filtrate for membrane extraction.

[0030] 3. Titanium alloy membrane separation device (with recoil system), membrane filtration pore size 0.1μm, multi-channel tubular membrane filtration area (0.25±0.05)m 2 . Equipped with a liquid storage tank of 40L, a titanium alloy membrane tube of 0.1 μm, a pH value of 7.0, an operating pressure of 1.2 MPa, an operating time of 40 min, a membrane su...

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Abstract

The invention relates to a process for separating and purifying natamycin out of a fermentation solution. The process comprises the steps of adjusting the pH value of the fermentation solution of the natamycin by an oxalic acid, adding yellow prussiate of potash and zinc sulfate sequentially, then adding sodium polyacrylate, and conducting nanofiltration purification, crystallization and vacuum drying after filtering and ultrafiltration purification through a chamber type membrane filter press so as to obtain the natamycin. According to the process, the extracting technology that ultrafiltration is conducted and then nanofiltration is conducted substitutes for traditional extracting technologies, the extracting cost is saved, the environmental pollution is reduced, the energy consumption is reduced, the process has the advantages of being simple in technology, high in yield (>=65%), high in purity (with the dry basis content>=95%) and low in cost, and the process is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for separating and purifying natamycin from fermentation liquid, belonging to the technical field of industrial microorganisms. Background technique [0002] Natamycin is a polyene, its molecule is an active cyclic tetraene compound, containing more than 3 crystal waters, its appearance is white (or cream), and it is an odorless crystalline powder, molecular formula C 33 h 47 NO 13 , the molecular weight is 665.73. Natamycin is an amphoteric substance with a basic group and an acidic group in its molecule. There are two typical configurations in its structure: enol structure and ketone structure, which determines its use in many Low solubility in solvents. In 1981, Brik pointed out that: at room temperature, the solubility of natamycin in water is 30-100mg / L. In water and lower alcohols, as the pH value increases and decreases, natamycin The solubility increases, the solubility is the lowest at neutral pH, and the...

Claims

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Application Information

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IPC IPC(8): C07H17/08C07H1/08
Inventor 杨旭锦石慧
Owner 北京明新高科技发展有限公司