Manufacturing method of pH ratio fluorescence probe based on organic dye-quantum dot compound

A ratiometric fluorescent probe, organic dye technology, applied in fluorescence/phosphorescence, luminescent materials, material excitation analysis, etc., can solve problems such as pH ratio fluorescent probes that have not yet been seen, and achieve the effects of fast detection, low cost, and simple method

Inactive Publication Date: 2013-01-09
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there have been no related patent reports on pH ratio fluorescent probes based on semiconductor quantum dots and pH ratio fluorescent probes based on organic dye-quantum dot composites

Method used

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  • Manufacturing method of pH ratio fluorescence probe based on organic dye-quantum dot compound
  • Manufacturing method of pH ratio fluorescence probe based on organic dye-quantum dot compound
  • Manufacturing method of pH ratio fluorescence probe based on organic dye-quantum dot compound

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Embodiment 1

[0024] The preparation process of pH ratiometric fluorescent probe based on FITC-CdTe complex can be found in figure 1 , the detailed preparation steps are as follows: first, use ultra-pure water to prepare the CdTe QDs solution (50nM) stabilized with thioglycolic acid, that is, in N 2 Atmosphere, magnetic stirring and alkaline aqueous solution, cadmium chloride, sodium telluride hydride, and thioglycolic acid are mixed in a molar ratio of 1:2:4, and the pH is adjusted to 10~12 by 1M NaOH solution, and the reaction is refluxed for 3~ After 6 hours, cadmium telluride quantum dots (CdTe QDs) stabilized with thioglycolic acid were prepared, and a certain amount of polyethyleneimine (PEI) was weighed and dissolved in ultrapure water, and the mass fraction was adjusted to 1%. Then, under room temperature and slow magnetic stirring, the PEI solution was added dropwise to the QDs solution, and PEI-modified QDs were formed by electrostatic adsorption, and the molar concentration of QD...

Embodiment 2

[0027] First, thioglycolic acid-stabilized CdTe QDs solution (50nM) was prepared in ultrapure water, that is, in N 2Atmosphere, magnetic stirring and alkaline aqueous solution, cadmium chloride, sodium telluride hydride, and thioglycolic acid are mixed in a molar ratio of 1:10:20, and the pH is adjusted to 10-12 by 1M NaOH solution, and the reaction is refluxed for 3- After 6 hours, cadmium telluride quantum dots (CdTe QDs) stabilized with thioglycolic acid were prepared, and a certain amount of polyethyleneimine (PEI) was weighed and dissolved in ultrapure water, and the mass fraction was adjusted to 1%. Then, under room temperature and slow magnetic stirring, the PEI solution was added dropwise to the QDs solution, and PEI-modified QDs were formed by electrostatic adsorption, and the molar concentration of QDs and the mass fraction of PEI were controlled to 40nM and 0.1%, respectively. Next, a certain amount of FITC was weighed and dissolved in ultrapure water, and the conce...

Embodiment 3

[0030] First, a thioglycolic acid-stabilized CdTe QDs solution (100 nM) was prepared in ultrapure water, and a certain amount of polyethyleneimine (PEI) was weighed and dissolved in ultrapure water, and the mass fraction was adjusted to 1%. Then, under room temperature and slow magnetic stirring, the PEI solution was added dropwise to the QDs solution, and PEI-modified QDs were formed by electrostatic adsorption, and the molar concentration of QDs and the mass fraction of PEI were controlled at 80nM and 0.1%, respectively. Next, a certain amount of FITC was weighed and dissolved in ultrapure water, and the concentration was adjusted to 150nM. At room temperature and under slow magnetic stirring, the FITC solution was added dropwise to the PEI-modified QDs solution to form a FITC-QDs composite by electrostatic adsorption. The molar concentrations of QDs and FITC were 60nM and 120nM, respectively. Finally, 10mM phosphate buffer solution (PBS) was used to adjust the pH of the com...

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Abstract

The invention relates to a manufacturing method of a pH ratio fluorescence probe based on an organic dye-quantum dot compound. The method comprises the following steps: first adopting polyethylenimine to modify a stable quantum dot (CdTe) of thioglycolic acid, then utilizing electrostatic interaction to enable fluorescein isothiocyanate (FITC) to be absorbed to the surface of the quantum dot, and obtaining a FITC-CdTe compound in an electrostatic-adsorption layer-upon-layer self-assembly mode. The fluorescence characteristic peak intensity ratio IFITC / ICdTe (or correspondingly I520 / I595) of the compound and pH are in linear relations, and the novel pH ratio fluorescence probe can be developed. Compared with the prior art, the manufacturing method is simple and low in cost, and the invented probe is high in sensitivity, can serve as a novel ratio fluorescence probe to be used for efficient detection of pH in biological samples and complex systems, and has important reference values for developing high-quality fluorescence probes of other types.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a preparation method of a pH ratio fluorescent probe based on an organic dye-quantum dot compound. Background technique [0002] The accurate measurement of pH value is of great significance to the research of physiology, pharmacology, environmental science, biology, chemistry and other systems. The conduct or completion of chemical reactions, many important processes of cells and organelles are closely related to pH. The existing pH measurement methods mainly include electrode method, indicator method and fluorescence spectrometry. The electrode method generally uses a glass electrode, which is not suitable for the pH detection of the living body due to electrochemical interference, possible mechanical wear and other defects. The indicator method generally uses pH indicators such as phenolphthalein and thymol, but the detection sensitivity and accuracy are low due...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/06G01N21/64
Inventor 万锕俊桂日军李慧丽金辉
Owner SHANGHAI JIAO TONG UNIV
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