Test strip and method for fast quantitative detection of drug in blood

A technology of quantitative detection and test strips, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of false positives, false negatives, and high sensitivity of urine samples

Inactive Publication Date: 2013-01-23
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, urine samples have their shortcomings: ①The analysis and detection of urine samples can only reflect the drug intake 1-3 days before sampling; ②Urine samples are relatively easy to falsify, for example, diluting urine samples can cause false negative results problems, artificially adding some ingredients, etc.; ③Due to the high

Method used

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  • Test strip and method for fast quantitative detection of drug in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Preparation and detection application of methadone colloidal gold detection test strip

[0028] 1.1 Preparation of colloidal gold-labeled methadone monoclonal antibody marker

[0029] Prepare a colloidal gold solution with a diameter of 40nm by chloroauric acid-trisodium citrate reduction method, and use 0.2MK 2 CO 3 The pH of the solution can be adjusted to pH 7-10, then the solution is placed on a magnetic stirrer and stirred slowly, and the methadone monoclonal antibody is slowly added to make the final concentration 5-15 μg / ml. Continue to stir for 1 hour, then add 0.2% sodium caseinate and 0.1% polyethylene glycol 20,000 to block for 30 minutes, centrifuge at 12,000 rpm for 30 minutes, discard the supernatant, redissolve to 76.5ml with colloidal gold working solution, press 1ml solution spread 16cm 2Spread evenly on the non-woven fabric, and then dry in a drying room at a temperature of 20-25°C and a humidity of less than 30% for 2-4 hours to make a...

Embodiment 2

[0039] Embodiment 2 Preparation and detection application of morphine latex method detection test strip

[0040] 2.1 Preparation of latex microsphere-labeled morphine monoclonal antibody marker

[0041] Take 1ml of red latex microspheres, add 9ml 0.1M pH6.5MES and mix well, add 0.75ml 10mg / ml NHS and 1ml 10mg / ml EDC·HCl, centrifuge for 20min, remove the supernatant, resuspend the precipitate with borax buffer, shake and sonicate After processing, the activated latex microspheres are formed. Dilute the morphine monoclonal antibody to 5mg / ml with 0.1M borax buffer (pH 8.5), take an appropriate amount of morphine monoclonal antibody and add 0.5ml activated latex microspheres, shake, centrifuge, remove the supernatant, and use 1% casein solution for precipitation Resuspend, ultrasonically pulverize and oscillate, repeat centrifugation once, remove the supernatant, resuspend the precipitate as above, ultrasonically pulverize and centrifuge again, and suspend the precipitate with a...

Embodiment 3

[0051] Example 3 Preparation and detection application of ketamine nano-immunomagnetic beads detection test strip

[0052] 3.1 Preparation of nano-magnetic bead-labeled ketamine monoclonal antibody marker

[0053] Take 10 mg of magnetic beads, prepare 1% nano-magnetic bead solution with 50 mM MES (pH5.7), add 0.5 mg of water-soluble EDC, 37 ° C for 10 min, then add appropriate amount of ketamine monoclonal antibody, incubate at 37 ° C for 2 hours, and then use The magnetic frame adsorbs the magnetic beads, and the buffer is exchanged to 0.01M PBS (pH7.2), containing 0.5% BSA, 0.5% Tween, and the labeling is completed. Then use the working solution to make a 0.5mg / ml solution, spread 22cm according to 1ml solution 2 Spread evenly on the glass fiber membrane and dry for 2-4 hours to make a nano-immunomagnetic bead-antibody marker pad for later use.

[0054] 3.2 Ketamine synthetic immune antigen coating

[0055] Dilute the morphine-synthesized immune antigen with 0.01M pH7.2PB...

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Abstract

The invention discloses a test strip and a method for fast quantitative detection of a drug in blood. The test strip comprises a sample loading pad, a marker pad, a NC membrane, a sample suction pad and a support thin sheet. The sample loading pad, the marker pad, the NC membrane and the sample suction pad are orderly adhered to the support thin sheet which does not absorb water. The marker pad is coated with marked drug-resistant monoclonal antibodies. The NC membrane is coated with a detection line T composed of drug synthesis immunizing antigens, and a quality control line C composed of goat anti-mouse IgG antibodies. The test strip can be operated simply, is accurate, can realize fast quantitation, has high sensitivity and specificity, is economic and practical, and is suitable for on-site detection.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a reagent strip and a detection method for rapidly quantitatively detecting drugs in blood. Background technique [0002] Drug addiction and drug abuse are social issues of global concern. According to WHO estimates, millions of people die from drug abuse and drug crimes worldwide every year, and it has become the third leading cause of death in many countries after cardiovascular and cerebrovascular diseases and malignant tumors. In recent years, under the influence of overseas drug activities, drug abuse and drug trafficking have once again affected my country, and various drug cases have shown a rapid upward trend, and the phenomenon of coexistence of transit drug trafficking and domestic consumption has formed. There are also more and more types of drugs. In addition to the common heroin, methamphetamine, ecstasy, and ketamine, there are also triazolam (Hiles...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/532G01N33/543
Inventor 李洲许俊艳
Owner 天津中新科炬生物制药股份有限公司
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