Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl.

A technology of artificial seeds, Dendrobium candidum, applied in the field of plant biology, to achieve the effects of increased proliferation efficiency, low leakage rate, and regular shape

Inactive Publication Date: 2013-01-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main problem to be solved by the present invention is the artificial seed production and storage technology of the rare medicinal plant Dendrobium candidum, which aims to effectively alleviate the situation of the depleti

Method used

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  • Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl.
  • Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl.
  • Method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl.

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one: the making and storage method of artificial seed of Dendrobium officinale

[0026] 1. Induction of Protocorms

[0027] Rinse the mature but uncracked capsules of Dendrobium officinale under tap water for 2 hours, put them into a clean bench, soak them in 75% alcohol for 0.5-1min, rinse them with sterile water for 3-5 times, and then soak them in 0.1% mercuric chloride solution 8-10min, wash 3-5 times with sterile water, place on sterile filter paper to absorb the surface moisture, cut the capsule with a scalpel, spread the seeds in the capsule evenly on the protocorm induction medium, and place it at 25℃ for artificial In the climate box, first carry out 5d dark cultivation, then transfer to light cultivation, the light intensity is 2000lx, the daily light time is 16h, after 15d of cultivation, the seeds germinate to form green protocorms. Protocorm induction medium consists of MS + 0.5mg / L naphthaleneacetic acid + 30g / L sucrose + 10wt% potato juice + 0...

Embodiment 2

[0034] Embodiment two: Orthogonal test of Dendrobium officinale artificial seed coat making

[0035]Using MS + 0.5mg / L naphthaleneacetic acid + 2.5mg / L 6-benzylaminopurine + 30g / L sucrose as artificial endosperm, add 2-4% sodium alginate to boil it into a semi-gel, and then autoclave it at 121℃ Cool down after 20 minutes for further use. On an ultra-clean workbench, evenly suspend protocorms of Dendrobium candidum with a size of 2-4 mm after 30 days of liquid suspension culture in the semi-gel, and use a dropper with an inner diameter of 4 mm to suck the semi-condensed Drop the glue and protocorm into the sterilized and cooled 50-150mmol / L calcium nitrate solution to ensure that each drop of semi-gel contains only one protocorm. After 20-30min ion exchange reaction, pour off the calcium nitrate solution and use Rinse three times with sterile water to obtain artificial seeds.

[0036] Sodium alginate concentration (2wt%, 3wt% and 4wt%), calcium nitrate concentration (50mmol / L,...

Embodiment 3

[0043] Example 3: Bacterial germination test of adding carbendazim to the artificial endosperm of Dendrobium officinale

[0044] Using MS + 0.5mg / L naphthaleneacetic acid + 2.5mg / L 6-benzylaminopurine + 30g / L sucrose + 0-0.5wt% carbendazim as artificial endosperm, add 3wt% sodium alginate to boil to make semi-coagulated The gel was sterilized under high pressure at 121°C for 20 minutes and then cooled for later use. On the ultra-clean workbench, the protocorm of Dendrobium officinale with a size of 2-4 mm after 30 days of liquid suspension multiplication culture was evenly suspended in the semi-gel. Take the semi-gel and protocorm with a 4mm dropper and drop them into the sterilized and cooled 100mmol / L calcium nitrate solution to ensure that each drop of semi-gel contains only one protocorm. After 25 minutes of ion exchange reaction, pour off the calcium nitrate solution, rinsed three times with sterile water to obtain artificial seeds. A total of 6 concentration (wt%) gradi...

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Abstract

The invention discloses a method for making and storing artificial seeds of Dendrobium candicum Wall.ex Lindl., which comprises the following steps: (1) inducing protocorm; (2) performing liquid suspension culture and proliferation of the protocorm; (3) making the artificial seeds; and (4) storing the artificial seeds. Compared with the prior art, the invention has the advantages that the protocorm of the Dendrobium candicum Wall.ex Lindl. is proliferated through liquid suspension culture (1/2MS medium), the proliferation efficiency is obviously improved compared with that of the conventional solid medium (MS medium), and massive high-quality artificial embryos are provided for artificial seed production; sodium alga acid and a calcium nitrate solution are subjected to ion exchange and curing to form artificial seed coats, and the prepared artificial seed coats have regular shapes and low nutrient leakage rate compared with those prepared by reacting the sodium alga acid and the calcium nitrate solution in the prior art; and the artificial seeds have long storage life and high germination rate.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for making and storing artificial seeds of dendrobium officinale. Background technique [0002] Dendrobium candicum Wall.ex Lindl., also known as black knot grass, is a perennial epiphytic herb of Orchidaceae. Dendrobium officinale has important medicinal value. It was first recorded in the "Shen Nong's Herbal Classic" in the Eastern Han Dynasty. It is used as a medicine with fresh or dry stems. "Great Immortal Grass" is the first among the people, and it is regarded as "life-saving immortal grass" by the people; "The Pharmacopoeia of the People's Republic of China" records that it can be used for yin injury and body fluid deficiency, dry mouth and polydipsia, insufficient stomach yin, lack of food, retching, and post-illness deficiency and heat Non-regression, hyperactivity of fire due to deficiency of yin, fatigue and heat due to steaming of bones, dark ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01F25/00
Inventor 赖佳李焕秀许轲唐懿秦耀国罗倩朱永鹏郑阳霞
Owner SICHUAN AGRI UNIV
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