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Rice salt stress related gene sidp364 and its encoded protein and application

A salt stress and rice technology, applied in rice salt stress related gene SIDP364 and its encoded protein and its application field, can solve problems such as agricultural production loss, achieve the effects of increasing food production, improving resistance, and effectively utilizing saline-alkali land

Active Publication Date: 2014-10-08
福建省生命之友生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the rapid increase of population in our country, the decrease of arable land and the shortage of fresh water resources, the area of ​​secondary salinized soil is still expanding, causing heavy losses to agricultural production.

Method used

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  • Rice salt stress related gene sidp364 and its encoded protein and application
  • Rice salt stress related gene sidp364 and its encoded protein and application
  • Rice salt stress related gene sidp364 and its encoded protein and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 The acquisition of rice salt stress-related gene SIDP364 and the construction of overexpression vector

[0036] A new gene family of unknown function, DUF1644, was identified from plants by bioinformatics analysis, which may play an important role in the stress response of rice as a new class of transcription factors. However, the relationship between DUF1644 family genes and stress resistance has not been reported yet. The gene SIDP364 is a member of the rice DUF1644 gene family.

[0037] According to the SIDP364 gene cDNA nucleic acid sequence, synthesize a pair of primers SIDP364-S and SIDP364-A:

[0038] SIDP364-S5'CACCATGGGTTCAGGAATGGTG3'

[0039] SIDP364-A5'CTAGTAGTATGAACGTCTGC3'

[0040] The TOPO cloning recognition site is introduced into the forward primer SIDP364-S, so that the gene DNA fragment can be cloned on the pENTR / D-TOPO vector by the TOPO cloning method.

[0041] Using the above primers and using the rice leaf cDNA as a template, PCR amp...

Embodiment 2

[0045] Example 2 Transformation of rice with rice salt stress-related gene SIDP364 gene overexpression vector and PCR detection and identification

[0046] Preparation of Agrobacterium suspension: Take 20 μL of Agrobacterium glycerol preservation solution containing pH7WG2-SIDP364 plasmid, inoculate it in 10 mL LB (containing 50 mg / L kanamycin and 50 mg / L rifampicin), and culture overnight at 28 °C with shaking. Collect the bacteria by centrifugation and resuspend with an appropriate volume of AAM culture medium to make the Agrobacterium suspension OD 600 The value is between 0.3 and 1, and finally add acetosyringone to make the final concentration reach 50mg / L.

[0047]Take the mature seeds of Taipei 309, after shelling, sterilize the surface with sodium hypochlorite solution (active chlorine is about 3%) with a volume ratio of 1 / 3, wash with sterile water for 2 to 5 times, and dry on sterile filter paper Afterwards, they were inoculated in the induction medium of NBD, and t...

Embodiment 3T1

[0055] Example 3 T1 generation SIDP364 gene overexpression SIDP364 gene expression in transgenic rice plants and analysis of its resistance to salt stress

[0056] Quantitative analysis of SIDP364 gene expression and identification of salt tolerance were carried out on two T1 generation overexpression transgenic plant lines and wild-type plants respectively. The seeds of T1 generation SIDP364 gene overexpression transgenic plants and wild-type rice seeds were sown respectively, and cultivated according to conventional cultivation methods. After the plants grew for two weeks, wild-type plants and two T1 generation SIDP364 gene overexpression Quantitative analysis of SIDP364 gene expression in transgenic plants. And identify the resistance of transgenic plants to salt stress.

[0057] The specific operation method is as follows:

[0058] An appropriate amount of leaves were taken from wild-type plants and SIDP364 gene overexpressed transgenic plants respectively, and total RNA...

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Abstract

The invention relates to a rice salt stress related gene SIDP364 and coding protein and application thereof, and relates to a rice gene. The invention provides a rice salt stress related gene SIDP364 and coding protein and application thereof. The rice salt stress related gene SIDP364 can be used for improving the resistance of rice to salt stress and cultivating rice with enhanced high-salt resistance. In a T1 generation overexpressed transgenic plant strain of the rice salt stress related gene SIDP364, the average values of the plant height and the biomass in environment of 200 mmol / L of high salt are higher than those of control obviously, so gene overexpressed transgenic plants can improve the resistance of the rice to the high-salt environment obviously. The gene provides an important path for the cultivation of the rice with the enhanced high-salt resistance. The cultivation of the rice with the high-salt resistance has the great significance on the effective utilization of saline-alkali soil, the increase of grain yield and the like.

Description

technical field [0001] The invention relates to rice genes, in particular to rice salt stress-related gene SIDP364 and its encoded protein and application. Background technique [0002] Agricultural production is an "open" large-scale production carried out in the natural environment, and often encounters unfavorable environmental conditions. When there are too many saline-alkali components in the soil, salt damage will occur. Salt damage is an important factor limiting agricultural production. According to statistics, currently about 20% of the world's cultivated land is threatened by salt damage. There are about 140 million mu of saline-alkali cultivated land in my country, mainly distributed in the northeast, north, northwest inland areas and coastal areas north of the Yangtze River. With the rapid increase of our population, the decrease of arable land and the shortage of fresh water resources, the area of ​​secondary salinized soil is still expanding, causing heavy los...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 陈亮李敏郭迟鸣罗成科张玉霞郭小玲
Owner 福建省生命之友生物科技有限公司
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