Method for extracting effective algae inhibition refinement component from barley

A barley, algae inhibitory activity technology, applied in the fields of botanical equipment and methods, herbicides and algicides, chemicals for biological control, etc., can solve the problem of not being able to determine whether there are substances that promote algal growth or some unknown substances , increase the risk of use, separation of active ingredients and other problems, to achieve the effect of good algae inhibition, low price and low preparation cost

Active Publication Date: 2013-02-13
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

[0005] In the patent applied by the applicant in 2011 (patent publication number: CN102415416A, titled: "A method for preparing a new type of algal bloom inhibitor from barley"), it is pointed out that the chloroform and ethyl acetate extracts of barley straw have It has obvious anti-algae activity, but the active ingredients of the extract are not further separated in this patent. Since the ingredients in the plant extract are relatively complex, it is impossible to determine whether there are substances that promote algae growth or some unknown substances. Increase the use of Risks, which will cause certain difficulties in the development of corresponding algastatic agents in the future

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  • Method for extracting effective algae inhibition refinement component from barley
  • Method for extracting effective algae inhibition refinement component from barley
  • Method for extracting effective algae inhibition refinement component from barley

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Embodiment 1

[0026] Firstly, the barley straw was dried at 50° C. for 96 hours, and the dried barley was crushed to 20 meshes. Soak 400g of dry barley with 2L of aqueous ethanol with 75% alcohol content, extract 5 times at 20°C for 2 hours each time, and combine to obtain the barley alcohol extract; put the barley alcohol extract at 40°C Distill the solvent under reduced pressure in the evaporator to obtain the extract A; extract the extract A twice with chloroform whose volume is 1-1.5 times that of water, and concentrate the extract of the chloroform phase to obtain the extract B, and the HPLC spectrum of the extract B See figure 2 ;

[0027] Secondly, the extract B was subjected to silica gel column chromatography, and the eluents were dichloromethane and methanol, and the volume ratios were 100:0, 49:1, 19:1, 19:1, 4:1 and 0:100 respectively. Gradient elution was carried out, the eluent was collected respectively and the solvent was removed, and the algae inhibitory activity test wa...

Embodiment 2

[0032] Firstly, the barley straw was dried at 70° C. for 48 hours, and the dried barley was crushed to 50 mesh. Soak 1Kg of dried barley with 4.5L of ethanol containing 40% alcohol, extract 2 times at 80°C for 6 hours each time, and combine to obtain the barley alcohol extract; put the barley alcohol extract at 50°C The solvent was distilled off under reduced pressure in a rotary evaporator to obtain extract A; extract A was sequentially extracted four times with an appropriate amount of dichloromethane, and extract B was obtained after concentrating the extract from the dichloromethane phase;

[0033] Secondly, the extract B was subjected to silica gel column chromatography, and the eluent was dichloromethane:methanol, and the volume ratios were 100:0, 49:1, 19:1, 10:1, 4:1 and 0:100 respectively. Gradient elution was carried out, the eluent was collected respectively and the solvent was removed, and the algae inhibitory activity test was carried out for each eluted component...

Embodiment 3

[0039] Firstly, the barley straw was dried at 60° C. for 36 hours, and the dried barley was crushed to 30 mesh. Soak 2Kg of dried barley with 8L of 70% ethanol in water, extract 2 times at 60°C for 6 hours each time, and combine to obtain the barley alcohol extract; put the barley alcohol extract at 40°C The solvent was distilled off under reduced pressure in a rotary evaporator to obtain extract A; extract A was sequentially extracted four times with an appropriate amount of ether, and extract B was obtained after concentrating the ether phase extract;

[0040] Secondly, the extract B was subjected to silica gel column chromatography, and the eluent was dichloromethane:methanol, respectively with volume ratios of 100:0, 49:1, 19:1, 10:1, 4:1 and 0:100. Gradient elution was carried out, the eluent was collected respectively and the solvent was removed, and the algae inhibitory activity test was carried out for each eluted component, and the results are shown in Table 3:

[00...

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Abstract

The present invention discloses a method for extracting an effective algae inhibition refinement component from barley. According to the method, barley straws are subjected to a pretreatment and extraction separation based on polarity to obtain an organic phase having algae inhibition activity, and the obtained extraction solution is completely dried and separated by using a positive direction silica gel column to obtain the refinement component having strong algae inhibition ability, wherein an eluant is prepared by mixing dichloromethane and methanol according to a volume ratio of 19:1. The method has characteristics of simpleness, easy performing, low cost, wide and easily-available materials and the like. With the method, the high purity barley allelopathy component having the algae inhibition activity can be obtained, and can be widely applicable for control of algae water blooms in lakes and reservoirs.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and particularly relates to a method for extracting effective algae-inhibiting subdivided components from barley. Background technique [0002] In recent decades, China's economy has developed rapidly. At the same time, a large amount of nutrient pollutants such as nitrogen and phosphorus have directly entered the water body, resulting in an imbalance in the water body ecosystem. Freshwater lakes such as Taihu Lake have a large area of ​​excessive growth of cyanobacteria, and this phenomenon is called "algae bloom". The outbreak of cyanobacterial blooms will seriously threaten the safety of urban drinking water supply and have a profound negative impact on human health and social and economic development. [0003] At present, the commonly used algae control methods are mainly source control, chemical control and physical control. Source control is to achieve the purpose of fundamenta...

Claims

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Application Information

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IPC IPC(8): A01N65/44A01P13/00A01P1/00
Inventor 肖溪陈英旭黄皓旻
Owner ZHEJIANG UNIV
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