Scenedesmus sp. and use thereof
A technology of Scenedesmus and cultivation method, which is applied in the field of microorganisms, can solve the problems of low processing capacity and performance, and achieve the effects of fast oil accumulation, high oil content, and fast growth speed
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Embodiment 1
[0037] Take a water sample from Dianchi Lake in Yunnan, add 1 / 3 volume of BG11 medium, culture for about 5 days at 25°C and light intensity of 40μmol / m2.s, take the water sample and observe under a microscope to judge the water sample The main species present in. Adjust the cell concentration to about 1000 cells / ml, take 100μl of water sample and spread it on the solid agar plate of BG11 medium, at 25℃, the light intensity is 40μmol / m 2 After culturing for about 10 days under the condition of about .s, you can see the colonies of single algae grow. Use an inoculating loop or a sterile toothpick to pick the colonies of single algae and inoculate them in BG11 medium. This process is repeated until a purely cultured algae strain is obtained, which is called "ENN34" in the present invention.
Embodiment 2
[0038] Example 2 Identification of algae species and their classification on the evolutionary tree.
[0039] The identification of algae strains adopts a combination of morphological identification and molecular identification.
[0040] 1. Morphological identification (see figure 1 ): Observe the isolated algae strain ENN34 under 1000 magnification. The cells are spherical, 6-10um in size, perichromosomal, a protein nucleus, and asexual reproduction produces parent-like spores. It was initially identified as Scenedesmus.
[0041] 2. Molecular identification:
[0042] A, Amplify its 18S nucleotide sequence from the isolated algae
[0043] The genomic DNA of ENN 34 algae cultured for 4 days was extracted by CTAB method. Take a certain amount of cells, add an appropriate amount of CTAB buffer, homogenize, add an equal volume of phenol: chloroform extraction, equal volume of isopropanol precipitation, wash with 75% ethanol and dissolve in a certain volume of sterilized double-distilled wa...
example 3
[0055] Example 3 Indoor cultivation, oil accumulation and pigment analysis of ENN34 algae:
[0056] The green cells of Scenedesmus strain ENN 34 in the logarithmic growth phase were inoculated in the prepared BG11 medium (see Table 1 for the formula), so that the cell density reached an OD750 of 0.2-0.8. During the cultivation process, the light intensity is controlled at 50-500umol / m2.s. During the cultivation period, the pH value of the medium is adjusted to 7-9 by passing 1.5-2% carbon dioxide and air mixed gas into the culture solution. In between, the temperature is regulated within the range of 15-35°C. The reactor used for the culture is a column reactor with an inner diameter of 40 mm and a length of 600 mm. During the cultivation process, samples are taken regularly to determine the dry weight, and the results are shown in Figure 4 . When the culture progresses to the 16th day, when the color of the algae liquid turns red, the algae liquid is collected, and the algae ...
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