Peanut skin extract for regulating activity of amylolytic enzyme and preparation method of extract

A starch decomposition and extraction technology, applied in the field of extracts, can solve the problems of undisclosed amylolytic enzyme inhibitory activity of peanut film extract

Inactive Publication Date: 2013-03-13
百岳特生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, none of the above-mentioned documents or studies revealed that

Method used

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  • Peanut skin extract for regulating activity of amylolytic enzyme and preparation method of extract
  • Peanut skin extract for regulating activity of amylolytic enzyme and preparation method of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The manufacturing process of embodiment 1 peanut film extract

[0019] 1.1 Preparation before peanut film extraction

[0020] A preferred embodiment of the present invention is to use No. 11 peanuts in Beigang, Tainan, Yunlin County for stripping. The peanuts go through an automatic shelling screen to remove clods and defective products, and then the peanuts are screened by a computer for thermal stripping (the temperature does not exceed 90°C. ), after the initial crushing by the machine, the peanut film is prevented from absorbing water and subpackaged, and immediately stored in ice at 4°C, which can effectively prevent the growth of aflatoxin, and the subsequent extraction will first check the aflatoxin content, which is in line with Taiwan's legal regulations The standard is to carry out after less than 15ppb. The detection of aflatoxin can be carried out according to the Taiwan Standard (CNS) General No. 4090, Class No. N6097 for the detection of aflatoxin in food...

Embodiment 2

[0025] The component content analysis of the powder that embodiment 2 peanut film extract concentrates and dries

[0026] The concentrated and dried powder of the peanut film extract obtained by the aforementioned preparation method of the present invention is further analyzed for the concentrated and dried powder of the peanut film extract, and its analysis method is as follows:

[0027] 2.1 Determination of total polyphenol content

[0028]The content of antioxidant total polyphenols was detected by Folin-Ciocalteu colorimetry, and the relative content of total polyphenols was expressed in terms of trihydroxybenzoic acid equivalent. Preparation materials include Folin-Ciocalteu reagent, 7.5% sodium carbonate and 1000 μg / mL trihydroxybenzoic acid (aqueous solution).

[0029] Prepare 1000 μg / mL trihydroxybenzoic acid standard solution of 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL and 100 μg / mL trihydroxybenzoic acid standard solution; take 100 μL of each concentration of trihydro...

Embodiment 3

[0050] Example 3 Analysis of Peanut Film Extract on α-amylase Inhibitory Ability

[0051] 3.1 α-amylase inhibitory ability analysis and preparation of materials before the experiment

[0052] The materials required for the analysis of α-amylase inhibitory ability include: 0.02M sodium phosphate buffer (containing 6mM sodium chloride, pH 6.9), 2N sodium hydroxide, dinitrosalicylic acid (osalicylic acid) reagent, 1% Soluble starch (starch) and α-amylase (α-amylase).

[0053] Dinitrosalicylic acid reagent (also known as terminator) preparation method: Take 1g of 3,5-dinitrosalicylic acid and add 50mL of deionized water, slowly add 30g of sodium potassium tartrate (sodium potassium tartratetetrahydrate), and then add 20mL 2N sodium hydroxide, quantitatively to 100mL with deionized water. Stored with carbon dioxide filling, the shelf life is limited to two weeks.

[0054] Preparation method of 1% soluble starch: Dissolve 1g of soluble starch in 100mL of 0.02M sodium phosphate bu...

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Abstract

The invention provides a peanut skin extract for regulating the activity of amylolytic enzyme. The extract is obtained by performing extraction and centrifugation on peanut skins with an extraction solvent, wherein the extraction solvent is water, alcohol, aqueous alcohol or a combination of the three, the regulation refers to inhibition of the activity, the amylolytic enzyme is alpha-amylase or alpha-glucosidase, and the extract is used for inhibiting amylolysis and slowing down the intestinal absorption of carbohydrates.

Description

technical field [0001] The invention provides an extract for regulating the activity of amylolytic enzymes, in particular a peanut film extract for regulating amylolytic enzymes and a preparation method thereof. Background technique [0002] When carbohydrates consumed by humans are starches, either linear or amylopectin can be rapidly decomposed by α-amylase in the salivary gland and pancreas. Amylase is a decomposing enzyme that hydrolyzes the α-1,4-glucosidic bonds linked in double-chain glucose. It can be divided into α-amylase and β-amylase according to the mode of action. α-amylase is an enzyme that hydrolyzes a large number of polysaccharides (such as starch and glycogen) connected by α-chains and α-chains to produce glucose and maltose; the type of α-amylase found in humans or other mammals is amylase ( saliva, pancreas, etc.), also occurs in starch-containing seeds as a food store, and many microorganisms secrete α-amylase. [0003] Similarly, α-glucosidase is a g...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/36A61K36/48A61P3/10A23L2/52C12G3/04A23L33/00A23L25/00
Inventor 苏香绫余锦秀林咏翔
Owner 百岳特生物科技(上海)有限公司
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