Use of nitrocarboxylic acids for treatment, diagnosis and prevention of aggressive healing patterns
A technology of nitrocarboxylic acid and nitro, applied in the field of internal prosthesis
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example 1
[0303] Example 1) Study for determining the effect of nitrocarboxylic acid on biological contamination and cell adhesion on prosthetic materials
[0304] Research on polymer stents (polyurethane, polyvinyl chloride, polylactate) used as implant materials. The solid and porous (pore size in the range of 50 to 150 microns) membrane of the pure polymer scaffold was cut into several pieces (5×5 mm). After cleaning with NaOH and ethanol, dip coating with untreated carboxylic acid and nitrated carboxylic acid. The dip-coated film was suspended in a tube filled with argon and heated at 60°C in the dark for 24 hours. Put the coated and uncoated membrane into the borosilicate glass tube, and fix the two edges of the membrane on the wall of the glass tube so that it is in an upright position in the center of the tube. Fill the tube with various solutions and keep it for 12 hours. The solution consists of the following: 0.9% normal saline, 2% bovine albumin, 2% bovine albumin with fibron...
example 2
[0312] Example 2) Evaluation of the effect of nitrocarboxylic acid on endothelial cell adhesion, reproduction and growth
[0313] A polystyrene scaffold was prepared and pre-treated in the same manner as performed in Example 1 by using the same nitrocarboxylic acid as listed in Table 1. The membrane sample is placed in a petri dish containing a gel matrix, in which human umbilical vein endothelial cells (HUVEC) have grown to confluence. The medium is composed of 5% FCS and is changed every 5 days. Perform cultivation according to standard conditions. On the 3rd, 7th, and 14th days, the membrane was carefully extracted from the petri dish, washed with physiological saline solution and stained with methylene blue to evaluate the membrane. Using a reflected light microscope, immediately inspect the membrane to evaluate the following: cell proliferation to the membrane center, cell confluence, multilayer formation, and cell shape.
[0314] The cells multiplied fastest on the uncoate...
example 3
[0316] Example 3) Evaluation of the effects of untreated fatty acids and nitro fatty acids on the mechanical changes of fibroblasts
[0317] In order to simulate the effect of chronic shear force on healing wounds, an in vitro model was established. Place a flat balloon on the bottom of a Petridish. Place the silicone plate on top and seal it with the side of the Petri dish. Subsequently, a 3 mm layer of agar was cast onto the top of the plate. Place commercially available polypropylene meshes for hernia repair (microporous mesh, low molecular weight macroporous mesh with absorbable polyglactin filaments, and high molecular weight microporous mesh) on the agar The plate is fixed at 4 points on the side of the Petri dish. This situation can stretch the mesh by filling the balloon with air, which is performed at 10 second intervals using an automatic suction device. The model can be used to evaluate the effect of three-dimensional (3D) shear force on cell growth during cell cul...
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Abstract
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