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Application of euphorbiaceous acid to biopesticides

A technology of euphemic acid and its uses, which is applied in the field of euphemic acid used in biopesticides, and achieves the effects of wide sources, expanded scope, and realization of diversification

Inactive Publication Date: 2013-03-27
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Euphorbia is one of the main chemical constituents of Euphorbia euphorbia, but no bactericidal and antibacterial research has been done on it

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take the leaves and leaf segments of the rice plant in the greenhouse, place them in a multi-well plate (24-well plate) cultured with water agar, and spray the leaves or leaf segments with test solutions of different concentrations in advance. After spraying, inoculate the pathogenic fungus blast oryzae, and after culturing for 9 days, calculate the inhibition rate based on the area of ​​the leaves not infected with the pathogenic bacteria. The inhibition rate of euphemic acid at a concentration of 200PPM was 20%.

Embodiment 2

[0016] Take the mycelium or spore suspension of the anthracnose pathogenic bacteria (made by mixing fresh liquid culture medium or preserved frozen liquid directly into the nutrient broth), take 20 μL of the test solution with a concentration of 20PPM into a 96-well plate, and then add it with The suspension of pathogenic bacteria was cultured in the dark at 24°C and 96% humidity. After 7 days, the fungal inhibition rate was calculated by measuring the optical density. The inhibition rate of euphoric acid at a concentration of 20PPM to anthracnose was 70%.

Embodiment 3

[0018] Take the mycelium or spore suspension of the pathogenic bacteria of wheat leaf spot (made by directly mixing fresh liquid culture medium or preserved frozen solution into nutrient broth), take 20 μL of the test solution with a concentration of 20PPM into a 96-well plate, and then Add the suspension with pathogenic bacteria, and carry out dark culture at 24°C and 96% humidity. After 7 days, calculate the fungal inhibition rate by measuring the optical density. The inhibition rate of 20PPM euphoric acid on wheat leaf spot is 100%.

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Abstract

The invention discloses application of euphorbiaceous acid to biopesticides and particularly relates to application of the euphorbiaceous acid to resistance to rice blast of plants, plant anthracnose, speckled leaf blotch of plants and wheat snow mould leaf blight of plants. The euphorbiaceous acid provided by the invention is capable of effectively inhibiting various plant pathogens, and the selection range of biological-source antibacterial agents is broadened.

Description

technical field [0001] The present invention relates to a new application of euphoric acid, in particular to the application of euphoric acid in pesticides. Background technique [0002] Epoxiconazole is a systemic triazole fungicide, its active ingredient epoxiconazole inhibits the synthesis of ergosterol of pathogenic bacteria and hinders the formation of bacterial cell walls. It can be used as a spectrum antibacterial agent and is widely used in pesticides. Cereal crop diseases such as blight, powdery mildew, eye striae and more than ten kinds of diseases have a good control effect. However, due to the complicated synthesis process, most of the domestic products rely on imports. Therefore, it is necessary to find a fungicide that has a wide range of sources, is easy to obtain, and can effectively replace epoxiconazole. [0003] Beijing Euphorbia, also known as Dragon Tiger Grass, General Grass, and Nine-Headed Lion, is the root of Euphorbiapekinensis Rupr., a perennial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/18A01P3/00
Inventor 马丽李维林王开梅顾玉诚刘玲玲
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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