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Method for preparing galactooligosaccharide co-produced ethanol

A technology of galactooligosaccharides and ethanol, which is applied in the field of preparation of galactooligosaccharides and co-production of ethanol, can solve the problem of high production cost of galactooligosaccharides, and achieve the effects of simplified separation costs, obvious economic benefits, and practical economic benefits

Active Publication Date: 2015-01-28
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned prior art, aiming at the problem of high production cost of galacto-oligosaccharides, the present invention provides a method for preparing galacto-oligosaccharides and co-producing ethanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 prepares galacto-oligosaccharide

[0016] The steps are as follows: Dry the anhydrous lactose overnight at 103°C, accurately weigh 30±0.1g of anhydrous lactose, make it up to 100mL, and prepare a 30% (w / v) lactose solution. After 5 minutes in a water bath at 45°C, add 0.5mL of β-galactosidase (enzyme activity: 4000IU / mL) expressed by Pichia pastoris was incubated at 45°C for 48 hours to obtain a mixed sugar solution. The solid content in the mixed sugar solution was determined by HPLC (solid content refers to the reaction The solid obtained after liquid drying) consists of: galactooligosaccharides, lactose, glucose and galactose, wherein the content of galactooligosaccharides is 35% (mass percentage, the same below), the content of lactose is 38%, and the content of glucose It is 24%, and the galactose content is 3%.

Embodiment 2

[0017] Embodiment 2 prepares galacto-oligosaccharide and co-produces ethanol

[0018] The steps are as follows: heat the mixed sugar solution obtained in Example 1 to 80°C and keep it warm for 15 minutes to inactivate the β-galactosidase, and then lower the temperature to 37°C; weigh 0.05g of Angelica high-temperature-resistant Saccharomyces cerevisiae (Ange Yeast Co., Ltd. Co., Ltd.), add 2mL 34℃ sterile water, shake and activate at 90rpm for 15min, add 0.05g urea and 0.05g potassium dihydrogen phosphate, and activated yeast to the mixed syrup, mix well, ferment at 37℃ for 48h, centrifuge at 6000rpm 10min, get supernatant, measure by HPLC, the composition of solid matter in the supernatant is: galacto-oligosaccharide, lactose, wherein, galacto-oligosaccharide content is 48%, and lactose content is 52% (glucose and galactose content are 0); the concentration of ethanol in the supernatant was 4° (%, v / v, the same below).

[0019] The supernatant is evaporated under reduced pre...

Embodiment 3

[0020] Embodiment 3 prepares galacto-oligosaccharide and co-produces ethanol

[0021] The steps are as follows: Dry the anhydrous lactose at 103°C overnight, accurately weigh 50±0.1g of anhydrous lactose, make it to 100mL, and prepare a 50% (w / v) lactose solution, bathe in water at 45°C for 5 minutes, add 0.25mL of β-galactosidase expressed by Pichia pastoris (enzyme activity is 4000IU / mL), incubate at 45°C for 48h, heat up to 80°C for 5min, cool down to 37°C, add 0.02g urea, 0.02g dihydrogen phosphate Potassium and 0.2g Angel’s high-temperature-resistant Saccharomyces cerevisiae (Ange Yeast Co., Ltd.), fermented at 37°C for 48 hours, obtained the fermentation liquid after fermentation, centrifuged at 6000rpm for 10min to get the supernatant, and collected the yeast cells, determined by HPLC, the supernatant The composition of solids in the liquid is: galacto-oligosaccharide, lactose, glucose, galactose, wherein the content of galacto-oligosaccharide is 51%, the content of lac...

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Abstract

The invention discloses a method for preparing galactooligosaccharide co-produced ethanol. The method comprises the following steps: mixing lactose solution; adding beta-galactosidase into the lactose solution to react for 2-48 hours; adding urea and monopotassium phosphate; putting in saccharomycetes to ferment and centrifuge; performing pressure reduction distillation on a supernate; condensing and collecting ethanol; and condensing the solution after the collection of the ethanol, namely syrup containing lactose and galactooligosaccharide; or condensing the solution after the collection of the ethanol, and spraying and drying to prepare powdered sugar. By adopting the method, prehydrolysis synchronous conversion fermentation and sub-step conversion fermentation modes are used, prehydrolysis is removed in a microorganism fermentation method to play a role in purifying galactooligosaccharide, and meanwhile, the ethanol is co-produced by glucose generated by performing hydrolysis on lactose by galactosidase and galactose. By adopting the method, the content of oligosaccharide in the galactooligosaccharide product can be improved, the purification cost is reduced, an additional value during the production of the galactooligosaccharide is increased, the separation cost is reduced, and the method has a practical economic effect.

Description

technical field [0001] The invention relates to a method for preparing galacto-oligosaccharide and co-producing ethanol. Background technique [0002] Galactooligosaccharides (GOS) is a functional oligosaccharide with natural properties. Its molecular structure is generally 1 to 7 galactose groups connected to galactose or glucose molecules, that is, Gal-(Gal)n -Glc / Gal (n is 0-6). In nature, there are trace amounts of GOS in animal milk, but more in human breast milk. The establishment of bifidobacteria in infants largely depends on the GOS components in breast milk. Galacto-oligosaccharides can selectively promote the proliferation of beneficial bacteria such as bifidobacteria inherent in the human intestinal tract, thereby inhibiting the growth of spoilage bacteria in the intestinal tract and reducing the formation of harmful metabolites. Therefore, it has the functions of preventing the occurrence of intestinal cancer, preventing constipation, It has the effects of low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/18C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 程少博夏蕊蕊肖林张文磊覃树林何凤云杨建李红震王艳君
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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