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Phosphorodiamidate morpholino oligomer (PMO) fluorescent nanoparticle for detecting mercury ion ratio and preparation method thereof

A fluorescent nanometer and mercury ion technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as complex preparation processes, and achieve the effects of easy repeatability, mild reaction conditions, and good biocompatibility

Inactive Publication Date: 2013-04-24
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, FRET ratiometric fluorescent probes need to covalently link the reference unit and the reporter unit of the probe, which requires careful molecular structure design and complicated preparation processes.

Method used

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  • Phosphorodiamidate morpholino oligomer (PMO) fluorescent nanoparticle for detecting mercury ion ratio and preparation method thereof
  • Phosphorodiamidate morpholino oligomer (PMO) fluorescent nanoparticle for detecting mercury ion ratio and preparation method thereof
  • Phosphorodiamidate morpholino oligomer (PMO) fluorescent nanoparticle for detecting mercury ion ratio and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Dissolve 9,10-dichloromethylanthracene (0.548g, 2mmol) in anhydrous acetonitrile (40mL) solution, then add anhydrous K 2 CO 3 (1.09g, 8mmol) and APTS (0.96mL, 4mmol), the mixed solution was refluxed under nitrogen protection for 48 hours. After the reaction was finished, the solution was cooled to room temperature, the organic solvent was removed, and the residue was purified by column chromatography (silica gel column, eluent was CH 2 Cl 2 ) to obtain a light yellow transparent oily liquid (0.20 g, yield 15%).

[0052]

[0053] (2) The preparation of PMO nanoparticles is through an improved Law. First, take cetyltrimethylammonium bromide (CTAB, 0.2g, 5.5×10 -4 mol) and NaOH (0.7mL, 2M) in a flask, add double distilled water (DDW) 100mL. The mixture was stirred for 15 minutes, then heated to 70°C, followed by the addition of 1.0 mL (0.94 g, 4.5 mmol) tetraethylorthosilicate (TEOS), 140 mg (0.225 mmol) disilylated anthracene and 0.1 mL (0.427 mmol) APTS. ...

Embodiment 2

[0061] Using the prepared material to detect mercury ions, different Hg 2+ The fluorescence spectra of PMO nanoparticles under concentration conditions are as follows: Figure 4 shown. (From bottom to fixed point, Hg 2+ The concentration is (1×10 -8 M~1×10 -4 M), as can be seen from this figure, when adding different amounts of mercury ions in the R6G / Anth-PMO (50mg / L) Hepes damping fluid of 7.0 when adding different amounts of mercury ions, the fluorescence emission intensity at 550nm is gradually enhanced, Figure 4 It shows that the PMO nanoparticle detector has a good detection performance for mercury ions.

[0062] Figure 5 is the ion selectivity of the PMO nanoparticle detector. It can be seen from the figure that an important feature of the modified PMO nanoparticles is its sensitivity to Hg 2+ exhibit high selectivity. Addition of most competing ions did not result in any appreciable change in fluorescence. However, when Hg 2+ After being added into aqueous s...

Embodiment 3

[0065] The PMO fluorescent nanoparticles used for mercury ion ratio detection are based on mesoporous silica and doped with two kinds of fluorescence resonance energy transfer dye molecules. One of the fluorescence resonance energy transfer dye molecules is sensitive to mercury ions. Another FRET dye molecule is stabilized to mercury ions.

[0066] Nanoparticles can emit fluorescence under the excitation of ultraviolet and visible light. Since there is a large overlap between the excitation and emission spectra of the two introduced organic fluorescent dye molecules, they can form a fluorescence resonance energy transfer dye pair, so they can be produced under excitation of a single wavelength. Two fluorescent emissions. In this example, the fluorescence resonance energy transfer dye molecule sensitive to mercury ions is a fluorescent molecule with a rhodamine 6G group with amino reactivity, and the fluorescence resonance energy transfer dye molecule stable to mercury ions is ...

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Abstract

The invention relates to a phosphorodiamidate morpholino oligomer (PMO) fluorescent nanoparticle for detecting mercury ion ratio and a preparation method thereof. The method comprises the steps of taking mesoporous silica as a carrier, and mixing with two fluorescent resonance energy transfer dye molecules; carrying out preliminary modification on an organic fluorescent molecule by a silane coupling agent, then utilizing a sol-gel chemical principle and self-assembly behaviors of a surfactant in solution as a structure-directing agent and silicon species, preparing and obtaining spherical PMO fluorescent nanoparticle by hydrolytic polycondensation between the silane coupling agent and a silicon source precursor; carrying out rhodamine dye modification on the PMO particle, and obtaining the PMO fluorescent nanoparticle for detecting the mercury ion ratio. Compared with the existing preparation route of a ratio probe, the preparation method has the main characteristics that a direct covalent bond connection between a reference signal unit and a report unit of the ratio probe is avoided; the molecular structure design of the ratio probe is simplified, and achievement of detection of different target materials is hopefully achieved by simply exchanging the reference signal unit and the report unit.

Description

technical field [0001] The invention belongs to the technical field of advanced nanometer materials and sensors, and in particular relates to PMO fluorescent nanoparticles used for mercury ion ratio detection and a preparation method thereof. Background technique [0002] Water pollution has become a global social and environmental problem. As a heavy metal ion, mercury ions have caused water pollution that has seriously threatened human health. Mercury ions are not only very toxic, but also difficult to degrade. Inorganic mercury ions will be converted into methylmercury under the action of microorganisms, so it is easy to find in rivers and lakes, and will accumulate toxins after being swallowed by fish and shrimp in the lake. After being transformed by the food chain, it gradually accumulates in the human brain, posing a serious threat to human health and life. Therefore, it is of great significance to detect and remove mercury ions in the environment. Fluorescence ana...

Claims

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Application Information

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IPC IPC(8): C09K11/06G01N21/64
Inventor 张金龙王灵芝陈宏
Owner EAST CHINA UNIV OF SCI & TECH
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