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Method for testing content of alkaloid in dendrobe

A dendrobium alkaloid and alkaloid technology, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of long measurement time, low yield of dendrobium alkaloids, and shortened measurement time, so as to achieve stable measurement results, shorten measurement time, The effect of improving sensitivity

Inactive Publication Date: 2013-05-01
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] Aiming at the currently commonly used bromocresol green acid dye colorimetric method for determining the alkaloids of Dendrobium with low yield and long time-consuming problem, the purpose of this invention is to provide an improved method for determining the content of alkaloids in Dendrobium to improve the biological efficiency of Dendrobium. The sensitivity of alkali determination, and can quickly stratify, shorten the determination time

Method used

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  • Method for testing content of alkaloid in dendrobe
  • Method for testing content of alkaloid in dendrobe
  • Method for testing content of alkaloid in dendrobe

Examples

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Effect test

Embodiment 1

[0015] Example 1 Take 0.2 g of dry dendrobium, grind it into powder, moisten it with 10 to 15 times the volume of concentrated ammonia water, seal it in a round-bottomed flask for 40 minutes, add 10 mL of chloroform, weigh it, and put it into the round-bottomed flask Put the condenser tube in a water bath at 80°C and heat to reflux for 2 h. Cool the reflux liquid in the round-bottom flask to room temperature and add chloroform to the original weight (due to the evaporation of chloroform due to heating and reflux), filter with qualitative filter paper, and then add chloroform to the filtrate to make up to 10 mL. Add 5 mL of 0.2M potassium hydrogen phthalate-sodium hydroxide buffer solution with pH 4.5 (that is, the concentration of potassium hydrogen phthalate and sodium hydroxide is 0.2M) and 0.04% bromocresol chloride solution by volume to the filtrate 1 mL, and add 0.4 mL Tween-80 aqueous solution with a volume ratio of 1:60 (1 volume of Tween-80: 60 volumes of water), shake ...

Embodiment 2

[0019] Example 2, the operation method is the same as that of Example 1, except that the effect of Tween addition on the determination of Dendrobium protocorm alkaloid content is determined by adding different volumes of Tween-80. The results are shown in Table 2.

[0020] Table 2 The effect of Tween addition on the determination of alkaloid content in Dendrobium protocorms

[0021]

[0022] As can be seen from Table 2, the 1:60 Tween-80 aqueous solution of different additions has great effect on Tween alkaloid content determination system

[0023] There were obvious effects, and the addition of 0.4 mL had the strongest sensitizing effect on the determination system of Dendrobium alkaloid content.

[0024] Taking the volume of the alkaloid extract as the abscissa, and the absorbance measured at the 620nm wavelength place as the ordinate, the regression equation is as follows:

[0025] the y 1 = 0.0685x + 0.0063R 2 = 0.9996

[0026] the y 2 = 0.0813x + 0.024R 2 = 0...

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Abstract

The invention discloses a method for testing the content of alkaloid in dendrobe. After a dendrobe dried product is crushed, alkaloid is extracted through alkaline chloroform water circumfluence, a nonionic surfactant Tween-80 is added into test liquid, and the content of alkaloid in the dendrobe is tested through bromocresol green acid dye colorimetry. In the system for testing alkaloid in dendrobe, a proper amount of Tween-80 is added to enable the extraction efficiency of bromocresol green-chloroform to be improved, so that the sensitivity in testing alkaloid in dendrobe is improved, and quick layering, simplicity and convenience in operation, good repeatability and stable testing result are achieved.

Description

technical field [0001] The invention relates to the field of quantitative determination of substance content by spectrophotometric colorimetry, in particular to a method for determining the content of dendrobium alkaloids. Background technique [0002] Alkaloids are a class of nitrogen-containing basic organic substances that exist in living organisms and have physiological activities. Plant alkaloids are a large class of secondary metabolites formed by plants to resist the attacks of animals, microorganisms, viruses and other plants during the long-term ecological environment adaptation process. Alkaloids are of great significance in the process of medicine, plant growth, development, symbiosis and reproduction. In my country, Chinese herbal medicines containing alkaloids were used for treatment as early as two or three thousand years ago. In "Shen Nong's Materia Medica", there are many kinds of Chinese herbal medicines containing alkaloids, such as top-grade Coptis chinen...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 金青林毅蔡永萍宋程孙旭
Owner ANHUI AGRICULTURAL UNIVERSITY
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