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Method for separating and purifying four phloroglucinol compounds from dryopteris fragrans

A technology of Trichosanthes serrata and phloroglucinol is applied in the field of separation and purification of plant active components, which can solve the problems of increased cost, time-consuming separation process, large loss of target compounds, etc., and achieves less pollution, reduced purification difficulty, and reduced practicality. Effect

Inactive Publication Date: 2013-05-15
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, in the traditional process of using plant extracts to separate and purify eidol, eidin PB, einequerin and einequerin BB monomers, the separation process takes a long time and the loss of the target compound is relatively large , resulting in higher cost

Method used

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  • Method for separating and purifying four phloroglucinol compounds from dryopteris fragrans
  • Method for separating and purifying four phloroglucinol compounds from dryopteris fragrans
  • Method for separating and purifying four phloroglucinol compounds from dryopteris fragrans

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Embodiment 1

[0018] Use dry Trichomonas as raw material, weigh 1000g, add 6 times the volume of warm water at 40°C for continuous induction for 3 times, each time for 8 minutes, adjust the pH of the homogenized induced liquid to 5.0 with citric acid; take 0.3% The compound enzyme (cellulase, pectinase) of the weight of the solid matter is dissolved in a buffer solution with a pH of 5.0, activated on a water bath at 50°C for 10 minutes, then mixed with the homogenate, and hydrolyzed at 40°C for 3 hours. After the separation of the induced liquid, the solid matter is extracted by microwave, using 80% ethanol as a solvent for extraction. The induced liquid and the ethanol extract are combined and concentrated, and then the solid matter is added to 1.5L of warm water at 50°C, and flocculated under the condition of 100KHz in an ultrasonic extractor. 15min, stand still for 10min, take viscous flocs, carry out negative pressure cavitation suspension solid extraction with equal volume of ethyl acet...

Embodiment 2

[0025] Use dried Trichophyllum as raw material, weigh 1000g, add 6 times the volume of warm water at 40°C for continuous induction for 3 times, each time for 10 minutes, adjust the pH of the homogenized induced liquid to 4.0 with citric acid; take 0.5% of The compound enzyme (cellulase, pectinase) of the weight of the solid matter is dissolved in a buffer solution with a pH of 4.0, activated on a water bath at 55°C for 10 minutes, then mixed with the homogenate, and hydrolyzed at 40°C for 4 hours. After the separation of the induced liquid, the solid matter is extracted by ultrasonic extraction method, using 80% ethanol as the solvent for extraction. The induced liquid and the ethanol extract are combined and concentrated, and the solid matter is added to 2L of warm water at 50°C, and vibrated and flocculated under the condition of 100KHz in the ultrasonic extractor for 20min. , stand still for 15 minutes, take the viscous floc, carry out negative pressure cavitation suspension...

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Abstract

The invention relates to a method for separating and purifying four phloroglucinol compounds from dryopteris fragrans, and aims at providing a safe, economical and effective method for separating and purifying monomers of aspidinol, aspidin PB, dryopteris fragrans element and aspidin BB. The method takes dry dryopteris fragrans as a raw material and comprises the following steps of: increasing the content of free active components by the homogenate induction technology and enzyme hydrolysis technology; performing ultrasonic-assisted extraction, quenching extraction, negative-pressure cavitation extraction and microwave-assisted extraction by taking ethanol as a solvent to obtain an extract; and performing ultrasonic oscillation flocculation technology, negative-pressure cavitation suspension solid extraction technology, macroporous resin column chromatographic enrichment technology, gel separation, normal-phase silica gel medium-pressure column chromatography isolation technology and low-temperature crystallization and recrystallization technology on the extract to obtain four monomers of aspidinol, aspidin PB, dryopteris fragrans element and aspidin BB, wherein the purity of the four monomers can exceed 95%. The method provided by the invention has the advantages that the separation and purifying technology is simple and easy to implement and safe to operate, the product yield is high, the purity is high and the dryopteris fragrans resource is rich and easily available, and is suitable for industrial application.

Description

technical field [0001] The invention relates to a method for separating and purifying four kinds of phloroglucinol compounds, eidinol, eidin PB, einequerin and einequerin BB from D. fern, belonging to the separation and purification technology of plant active components field. Background technique [0002] Dryopterisfragrans (L.) Schott.] is a perennial fern, belonging to the genus Dryopteris fragrans, also known as Dryopteris fragrans (L.) Schott. It is mainly distributed in Northeast my country, North China, Central China, Russia (Far East), North Korea, Japan and some countries in North America. Hair fern has high medicinal value, and it is commonly used by folks to treat various skin diseases (such as psoriasis, rash, dermatitis, acne, etc.). It is "the nemesis of skin diseases" (Shen Zhibin et al., 2002). In addition, Dermascens also has obvious therapeutic effect on rheumatoid arthritis (Chang Ying, 2009). In recent years, a number of pharmacological research result...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C49/84C07C49/723
Inventor 付玉杰高畅李娜李吉赵春健李春英张东阳
Owner NORTHEAST FORESTRY UNIVERSITY
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