Application of sericin peptide in freeze drying or refrigeration preserving probiotics

A technology of probiotics and sericin peptides, applied in the preservation of microorganisms, etc., to achieve high nutritional and health value, inhibit tyrosinase, and enrich the effect of physiological functions

Inactive Publication Date: 2013-05-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research and report on using sericin peptide directly as probiotic freeze-drying protective agent

Method used

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  • Application of sericin peptide in freeze drying or refrigeration preserving probiotics
  • Application of sericin peptide in freeze drying or refrigeration preserving probiotics
  • Application of sericin peptide in freeze drying or refrigeration preserving probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Select Bifidobacterium bifidum and Lactobacillus bulgaricus, mix them at 1:1 (mass ratio), inoculate them into sterilized 120g / L skim milk medium for fermentation, the fermentation temperature is 42°C, the fermentation time is 4h, and the resulting pH is 4.5, the bacteria content is 2.16×10 8 CFU / mL of fermented milk, adding different amounts of sericin peptide to the fermented milk, more than 85% of the components in sericin peptide have a relative molecular weight distribution below 3000Da, purchased from Huzhou Xintiansi Biotechnology Co., Ltd. to make sericin peptide The mass percentage concentration (W / W) in fermented milk reached 0, 0.6%, 1.2%, 1.8%, 2.4%, 3.6% respectively, and then frozen at -20°C for 12 hours, at -50°C, the vacuum degree was Freeze-drying under the condition of 10pa for 16 hours, to prepare a probiotic bacterial agent rich in Lactobacillus bulgaricus and Bifidobacterium bifidum in the state of freeze-dried powder.

[0043] The number of viable...

Embodiment 2

[0045] Select Lactobacillus bulgaricus, inoculate it into M17 broth culture medium, cultivate overnight at 37°C, 180rpm, until the absorbance value at 600nm is 1.2, then inoculate it in M17 broth at a concentration of 10% by volume and cultivate it for 4h, 4 Centrifuge at 6000 rpm for 10 min to collect the sludge. Suspend the bacteria slime with the same volume of sterilized deionized water as the original culture medium, and mix 0.5 mL of the bacteria suspension with 0.5 mL of 10 mg / mL sericin peptide solution. The mixed bacterial suspension samples were stored at -20°C for 22 days. Regularly check the survival rate of bacteria during the frozen storage process, freeze and thaw once before each test, and thaw in a water bath at 37°C for 10 minutes during the dissolution process. Survival rate of Lactobacillus bulgaricus during frozen storage see figure 2 .

[0046] Depend on figure 2 It can be seen that within the first 2 days of frozen storage, the Lactobacillus bulgar...

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Abstract

The invention relates to a novel probiotics freeze drying protective agent sericin peptide and an application. The sericin peptide is a product obtained by sericin through enzyme hydrolysis preparation, the relative molecular weight enables wide distribution in 10000Da-300Da scope, an ingredient with superior activity is mainly distributed below 3000Da, the amino acid composition characteristic is characterized in that the content of amino acid with hydroxy such as serine and threonine can reach as high as 39%. The sericin peptide for freeze drying or freeze preservation of Bifidobacterium bifidum and Lactobacillus bulgaricus has high freeze-drying or refrigeration protection rate, and has stable protection effect. The application has the advantages that the sericin peptide is taken as a freeze drying or freeze preservation protective agent of the probiotics, the application of sericin peptide taken as the natural freeze drying protective agent with nutrition and health care functions can be widen.

Description

technical field [0001] The invention relates to the frozen storage technology of probiotics, in particular to a protective agent used in freeze-drying or frozen storage of probiotics, and belongs to the technical field of microorganisms in food engineering. Background technique [0002] Probiotics refer to bacteria that improve the host's micro-ecological balance and play a beneficial role in improving the host's health level and health status. They can be directly taken as food additives to maintain the balance of intestinal flora. Probiotics have rich physiological functions such as: enhancing human immunity; inhibiting the reproduction of harmful bacteria in the intestines, reducing toxins, promoting intestinal peristalsis, improving defecation conditions, thereby improving intestinal function and intestinal digestive system health; helping vitamin B 1 , B 2 , B 6 Absorption of other nutrients; lower serum cholesterol, etc. Therefore, probiotics are an important health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
Inventor 吴金鸿周炎富王正武何亚雯姚芳懿荣玉芝
Owner SHANGHAI JIAO TONG UNIV
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