Kit for detecting tetracycline drug and detection method therefor

A technology of tetracycline drugs and kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation and high pretreatment requirements, and achieve the effect of simple operation, short detection time and guaranteed reliability

Active Publication Date: 2013-05-15
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The reported detection methods of tetracycline drug residues include microbiological method, thin layer chromatography, high performance liquid chromatography, high performance liquid chromatography-tandem mass spectrometry, capillary electrophoresis, electrochemical analysis, chemiluminescence, enzyme-linked immunoassay, colloidal Gold immunoassay, etc., the instrument detection method requires high pretreatment of the sample to be tested, the operation is cumbersome, and precision equipment is required

Method used

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  • Kit for detecting tetracycline drug and detection method therefor
  • Kit for detecting tetracycline drug and detection method therefor
  • Kit for detecting tetracycline drug and detection method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 detects the composition of the test kit of tetracyclines

[0044] 1. Test paper (called test strip) ( figure 1 )

[0045] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0046] The sample absorbent pad 1, the reaction film 2, the water absorbent pad 3 and the protective film 7 are pasted on the bottom plate 6 in sequence, the end of the sample absorbent pad is connected with the reaction film, the end of the reaction film is connected with the water absorbent pad, and the sample absorbent pad The beginning is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0047] The end of the test paper sample absorption pad is pasted with a protective film, and the protective film 7 covers the detection end on the sample absorption pad, and the word MAX is printed on the detection end protective film (...

Embodiment 3

[0108] The detection of tetracyclines in the milk sample of embodiment 3

[0109] 1. Detection with kit

[0110] Take out the reagent barrel from the original package (if it is stored at low temperature, it needs to be pre-balanced to room temperature), open it, take out the required number of micropore reagents and test strips, and mark them; draw the milk sample to be tested, and pipette it with a micropipette. Take 200 μl in the microwell, slowly suck and mix well with the reagent in the microwell, incubate at room temperature (20°C-25°C) for 5 minutes; insert the marked test strip into the microwell - printed with "MAX" line The end is facing down, so that it is fully immersed in the solution; after incubating at room temperature (20°C-25°C) for 5 minutes, take out the test strip and judge the result.

[0111] 2. Analysis of test results ( Figure 4 )

[0112] Positive: When the quality control area (C) shows a band, but the detection area (T) does not display, it is judge...

Embodiment 4

[0113] The determination of embodiment 4 kit technical parameters

[0114] 1. Sensitivity test

[0115] Dilute tetracycline, doxycycline, chlortetracycline, oxytetracycline standard (purchased from Sigma) to the following concentrations: tetracycline 0, 20, 40, 80 μg / L, doxycycline 0, 20, 40, 80 μg / L , chlortetracycline 0, 20, 40, 80 μg / L, oxytetracycline 0, 40, 80, 160 μg / L; the diluent used is phosphate buffer with pH 7.2 and 0.2mol / L.

[0116] Tested with the kit, the results are: when the concentration of tetracycline standard substance is 0, 20 μg / L, the concentration of doxycycline standard substance is 0, 20 μg / L, the concentration of aureomycin standard substance is 0, 20 μg / L, oxytetracycline When the concentration of the standard substance is 0, 40 μg / L, two red lines visible to the naked eye are displayed on the test strip, which is negative; When the concentration of chlortetracycline standard substance is 40 and 80 μg / L, and the concentration of oxytetracycline ...

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Abstract

The invention discloses a kit for detecting a tetracycline drug and a detection method therefor. The kit comprises a microporous reagent and a test paper. The microporous reagent contains freeze-dried colloidal gold-labeled tetracycline drug specific antibodies. The tetracycline drug specific antibodies are tetracycline drug monoclonal antibodies or tetracycline drug polyclonal antibodies. The test paper comprises a sample adsorption pad, a reaction film, a water adsorption pad, a protective film and a base plate, wherein the sample adsorption pad, the reaction film, the water adsorption pad, the protective film and the base plate are connected orderly. The reaction film comprises a detection zone and a quality control zone. The detection zone is coated with a tetracycline hapten-carrier protein conjugate. The quality control zone is coated with antiantibodies. The detection method suitable for the kit has simple processes, realizes fast, visual and accurate detection, has a wide application range and a low cost, and can be used and popularized easily.

Description

technical field [0001] The invention relates to a test kit and a method for detecting tetracycline drugs, in particular to a colloidal gold test strip for detecting tetracycline drugs in milk samples and a detection method thereof. technical background [0002] Tetracyclines (TCs) have broad-spectrum and high-efficiency bactericidal and antimicrobial effects, and can be mixed with most drugs or feed additives, and have been widely used in livestock and poultry production. However, excessive use inevitably causes serious drug residues, which has become one of the food safety hazards that seriously endanger human health. For this reason, countries all over the world pay great attention to the detection of TCs residues. EU Directive No. 675 / 92 preliminarily stipulates that the total amount of tetracyclines in muscle and milk shall not exceed 100 μg / kg, and that in kidney, liver and eggs shall not exceed 600 μg / kg, 300 μg / kg and 200 μg / kg respectively. Japan's positive list sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 万宇平冯才伟孙震冯静冯月君余厚美罗晓琴刘福林刘琳张荃
Owner BEIJING KWINBON BIOTECH
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