Liquid-chromatogram and tandem-mass-spectrogram assay method for CEMA (Cyanoethyl Methacrylate) and HEMA (Hydroxyethyl Methacrylate) in urine
A measurement method, tandem mass spectrometry technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve good sensitivity and repeatability, reduce errors, and high sensitivity
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example 1
[0048] 1. Instruments and reagents: Agilent 1200 fast high-resolution liquid chromatography (Agilent, USA), equipped with G1367D autosampler, G1312B binary mixing pump and G1316B column thermostat; API 5500 triple quadrupole tandem mass spectrometer (Applied Biotechnology, USA) Systems Company), equipped with electrospray ion source and analyst 1.5 software data processing system.
[0049] N-acetyl-S-(2-cyanoethyl)-L-cysteine (purity 98%), N-acetyl-S-(2-hydroxyethyl)-L-cysteine ( Purity 98%), N-acetyl-S-(2-cyanoethyl)-L-cysteine-D3 (chemical purity 98%, isotopic purity 99%), N-acetyl-S-(2 -Hydroxyethyl)-L-cysteine-D4 (chemical purity 98%, isotopic purity 99%) (Toronto Research Chemicals). Formic acid (chromatographically pure, Acros Organics). Methanol and acetonitrile (chromatographically pure, TEDIA company).
[0050] 2. Sample handling: Thaw urine at room temperature and mix well. Accurately pipette 1.0 mL of urine, add 10 μL of formic acid to acidify, and centrif...
example 2
[0052] Example 2: As described in Example 1, 37 urine samples were taken. The content of CEMA and HEMA in urine was measured as image 3 shown.
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