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Three-dimensional controllable incremental forming method and forming system for active osteochondral integrated gradient scaffold

An incremental forming, osteochondral technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of unfavorable tissue or organ formation, difficult to achieve controllable positioning and distribution of cells, unable to meet the three-dimensional balanced growth of cells, etc., to achieve three-dimensional The effect of three-dimensional shaping

Inactive Publication Date: 2013-06-12
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this culture mode, cells can only grow in one direction from the surface of the scaffold to the inside, which cannot meet the requirements of three-dimensional balanced growth of cells in natural tissues; Facilitates tissue or organ generation with functional gradient requirements

Method used

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  • Three-dimensional controllable incremental forming method and forming system for active osteochondral integrated gradient scaffold
  • Three-dimensional controllable incremental forming method and forming system for active osteochondral integrated gradient scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] see figure 1 , the three-dimensional controllable incremental forming method of the active osteochondral integrated gradient scaffold is characterized in that the operation steps are as follows:

[0025] 1) The ion concentration-sensitive material and the gelation-promoting natural biological material were prepared into aqueous solutions with a mass fraction of 3.6-4.4% and 18-22%, respectively, and were sterilized for later use;

[0026]2) Fully mix the seed cells with the prepared ion concentration-sensitive material and the aqueous solution of the gelation-promoting natural biological material according to the mass ratio of 1:5:2.5~1:10:10, and prepare a gel-like cell-biological material Mixing unit; the synthetic polymer biomaterial is sterilized and heated to a molten state; the cell-biomaterial mixing unit and the molten polymer biomaterial are poured into different barrels; according to the pre-designed scaffold structure and forming path, Under the control of t...

Embodiment 2

[0030] This example is basically the same as Example 1, and the special feature is: the three-dimensional controllable incremental forming method of the active osteochondral integrated gradient scaffold, the ion concentration-sensitive material in the step 1) is sodium alginate, The natural biomaterial that promotes the gelation transition of the material is gelatin, and the synthetic polymer biomaterial is polylactic-co-glycolic acid (PLGA) with a molecular weight of 15,000; the steps 2) and 3 ) in the integrated gradient scaffold, the cartilage layer scaffold with small three-dimensional through hole size is composed of gel state cell-biomaterial hybrid unit, the three-dimensional through hole size is 100~150 μm to facilitate cartilage generation; the three-dimensional through hole size is large The subchondral bone layer scaffold is composed of core materials: the main material of the inner layer is PLGA, and a layer of cell-biological material mixed unit is wrapped on the s...

Embodiment 3

[0032] see figure 2 , the three-dimensional controllable incremental forming system of the active osteochondral integrated gradient scaffold is applied to the three-dimensional controllable incremental forming method of the active osteochondral integrated gradient scaffold to realize forming, including: three-dimensional motion system (1), Coaxial nozzle extrusion unit (2), air pump (3), support material receiving plate (4), water bath (5), computer control system (6) and barrel (7), characterized in that: the air pump ( 3), the coaxial nozzle extrusion unit (2), and the barrel (7) are installed on the three-dimensional motion system (1), above the water bath (5), and can realize X-Y plane motion; the bracket material receiving plate (4) and The three-dimensional motion system (1) is connected to move in the Z direction, and the receiving plate (4) of the support material is placed in the water bath (5); the computer control system (6) is connected to the three-dimensional mo...

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Abstract

The invention relates to a three-dimensional controllable incremental forming method and a three-dimensional controllable incremental forming system for an active osteochondral integrated gradient scaffold. According to the method, a natural biomaterial and seed cells are fully mixed to form cell-biomaterial active gel which is used as a main body material of a chondral layer and a surface modification material of a chondral lower bone layer in the integrated active scaffold, and a synthetic macromolecular biomaterial with good mechanical property and controllable degradability is used as a main body material of a chondral lower bone layer scaffold. The method comprises the following steps of: extruding the active bio-gel and the molten synthetic macromolecular biomaterial by coaxial nozzles according to the preset scaffold structure and forming path, controlling start and stop of feed power for the synthetic macromolecular biomaterial through a computer, performing layer-by-layer incremental forming, and thus obtaining the osteochondral integrated gradient scaffold with good mechanical property and bioactivity. The integrated gradient scaffold with the bioactivity can realize three-dimensional controllable forming of cells, meanwhile, good mechanical property of the scaffold can be ensured, and the scaffold with controllable pore structure and degradation rate is beneficial to three-dimensional culture of the cells and tissue regeneration.

Description

technical field [0001] The invention relates to a three-dimensionally controllable incremental forming method and a forming system of an active osteochondral integrated gradient support. The invention belongs to the field of tissue engineering and cartilage tissue repair. Background technique [0002] Defects or injuries of joint tissue caused by trauma, sports injury, inflammation and tissue degeneration are very common in clinical practice. Because there are no blood vessels, nerves, and lymphatic systems in cartilage tissue, chondrocytes are confined in dense lacunas composed of collagen and proteoglycans, and their own metabolic activity is low, so the ability of articular cartilage regeneration and self-repair is extremely limited. Once the articular cartilage is damaged, without proper treatment, the damaged cartilage will not be repaired by the hyaline cartilage, but may cause subchondral bone damage due to aggravated wear and tear, and develop into bone join...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/38A61L27/14A61L27/50A61L27/56A61L27/20A61L27/22A61L27/18
Inventor 于永泽刘莹韩振中刘媛媛胡庆夕
Owner SHANGHAI UNIV
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