Scenedesmus strain and application thereof

A technology of Scenedesmus strains and Scenedesmus, which is applied in the field of microbial engineering, can solve the problems of growth and collection characteristics of unstudied algae strains, and achieve the effects of easy high-density cultivation, strong environmental adaptability, and cost reduction

Active Publication Date: 2013-06-19
ENN SCI & TECH DEV
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AI Technical Summary

Problems solved by technology

[0005] The screening of a strain of Scenedesmus obliquus published by Ji Xiang, Wang Jinrong, etc. and the optimization of growth conditions (Aquaculture Science and Technology Information 201037 (6)) disclose the algal strain of Scenedesmus, in taxonomy with the Scenedesmus sp. The strains belong to different genera, and the oil content rate is 31.5%. This paper does not study the growth and collection characteristics of the algae strains under special environmental conditions.

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  • Scenedesmus strain and application thereof
  • Scenedesmus strain and application thereof
  • Scenedesmus strain and application thereof

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Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1 screening oil-producing Scenedesmus strains

[0040] Take the river water sample taken back from the Langfang area diluted with sterile water. After observation under a 400-fold microscope, the approximate cell concentration is 800-1200 cells / ml. Take about 1ul of the algae liquid with a capillary tube and inoculate it in 48 wells equipped with In the culture plate of BG11 medium (see Table 1), at 25 ℃, light intensity is 50umol / m 2 .s in the case of cultivation, when it reaches 10 7 / ml cell concentration, observed under microscope, selected wells with only single algae strain, diluted to cell concentration of 1000-3000 cells / ml, plated, obtained single algae strain and stored on BG11 solid plate, named ENN2203A.

[0041] Table 1BG11 medium formula

[0042] NaNO 3

1.5g / L

K 2 HPO 4 ·3H 2 o

0.04g / L

MgSO 4 ·7H 2 o

0.075g / L

CaCl 2 2H 2 o

0.036g / L

citric acid

0.006g / L

FeCl 3 ·6H...

Embodiment 2

[0045] Embodiment 2 determines the classification of algal species on the phylogenetic tree

[0046] The identification of algae strains adopts the combination of morphological identification and molecular identification.

[0047] (1) Morphological identification:

[0048] The above-mentioned isolated algal strain ENN2203A was observed under a 1000 times microscope (see figure 2 ), the algal body is a single cell or a stereotyped group consisting of 2 or 4 single cells, the single cell is spherical or oval, and 2 or 4 single cells in the stereotyped group composed of 2 or 4 single cells are arranged in a straight line One column, the upper and lower ends of each cell are wide and round, the cell wall is smooth, each cell has a long spine that bends outwards at the upper and lower ends, and two short spines in the middle of the outer wall; a stereotyped population composed of 4 single cells The colony is 15-20 μm wide, each single cell is 5-8 μm long and 3-4 μm wide; the chro...

Embodiment 3

[0063] Indoor culture and oil accumulation of embodiment 3ENN2203A algae

[0064] The green swimming cells of Scenedesmus strain ENN2203A in the logarithmic growth phase were inoculated in the prepared BG11 medium (see Table 1 for the formula) to make the cell density reach OD 750 between 0.2-0.8; the light intensity during the cultivation process is controlled at 50-500umol / m 2 .s, during the culture period, adjust the pH value of the culture medium between 7-9 and the temperature within the range of 15-35°C by feeding a mixture of 1.5-2% carbon dioxide and air into the culture medium The reactor used for cultivating is a column reactor with an internal diameter of 40mm and a length of 600mm; image 3 On the 16th day of cultivation, the final dry weight reached 10.93g / L. At this time, the color of the algae liquid turned yellow, and the algae liquid was collected, and the algae mud was obtained by centrifugation or natural sedimentation, and the algae mud was vacuum freeze-d...

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Abstract

The invention provides a scenedesmus strain (Desmodesmus sp.) and application of the scenedesmus strain. The scenedesmus strain is ENN2203A and stored in the general microbe center of China microbe culture preservation management council on November 11, 2012. The preservation number is CGMCC NO.4323. The scenedesmus strain has the advantages of being high temperature resistant, easy to collect, strong in environmental suitability, suitable for high density cultivation, and capable of being used for treatment of sewage containing nitrogen and phosphorus and carbon dioxide emission reduction, and is used for biodiesel production or used for baits of an aquatic product or feeding stuff of animal. Industrialization utilization potential is relatively high.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular, the invention relates to a Scenedesmus strain (Desmodesmus sp.) and its application. Background technique [0002] The increasingly serious energy shortage and environmental degradation have become the focus of attention all over the world. Fossil fuels such as oil, natural gas, and coal are the main energy sources today. This type of energy is non-renewable. With the continuous consumption of this type of energy, its natural reserves will decrease sharply. The time for human use is about 45-50 years for oil, 50-60 years for natural gas, and 200-220 years for coal. Combustion of fossil fuels produces large amounts of water and carbon dioxide, which is the main cause of global warming. Many scientists believe that the burning of coal, oil, natural gas and trees produces a large amount of carbon dioxide, which will heat up the earth after entering the atmosphere. The increase i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C02F3/34B01D53/84B01D53/62A23K1/00C12R1/89
CPCY02A50/20Y02E50/10Y02P20/59
Inventor 王媛媛邓平吴义诚马建源邵元龙吴洪
Owner ENN SCI & TECH DEV
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