Scenedesmus strain and application thereof
A technology of Scenedesmus strains and Scenedesmus, which is applied in the field of microbial engineering, can solve the problems of growth and collection characteristics of unstudied algae strains, and achieve the effects of easy high-density cultivation, strong environmental adaptability, and cost reduction
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Embodiment 1
[0039] Embodiment 1 screening oil-producing Scenedesmus strains
[0040] Take the river water sample taken back from the Langfang area diluted with sterile water. After observation under a 400-fold microscope, the approximate cell concentration is 800-1200 cells / ml. Take about 1ul of the algae liquid with a capillary tube and inoculate it in 48 wells equipped with In the culture plate of BG11 medium (see Table 1), at 25 ℃, light intensity is 50umol / m 2 .s in the case of cultivation, when it reaches 10 7 / ml cell concentration, observed under microscope, selected wells with only single algae strain, diluted to cell concentration of 1000-3000 cells / ml, plated, obtained single algae strain and stored on BG11 solid plate, named ENN2203A.
[0041] Table 1BG11 medium formula
[0042] NaNO 3
1.5g / L
K 2 HPO 4 ·3H 2 o
0.04g / L
MgSO 4 ·7H 2 o
0.075g / L
CaCl 2 2H 2 o
0.036g / L
0.006g / L
FeCl 3 ·6H...
Embodiment 2
[0045] Embodiment 2 determines the classification of algal species on the phylogenetic tree
[0046] The identification of algae strains adopts the combination of morphological identification and molecular identification.
[0047] (1) Morphological identification:
[0048] The above-mentioned isolated algal strain ENN2203A was observed under a 1000 times microscope (see figure 2 ), the algal body is a single cell or a stereotyped group consisting of 2 or 4 single cells, the single cell is spherical or oval, and 2 or 4 single cells in the stereotyped group composed of 2 or 4 single cells are arranged in a straight line One column, the upper and lower ends of each cell are wide and round, the cell wall is smooth, each cell has a long spine that bends outwards at the upper and lower ends, and two short spines in the middle of the outer wall; a stereotyped population composed of 4 single cells The colony is 15-20 μm wide, each single cell is 5-8 μm long and 3-4 μm wide; the chro...
Embodiment 3
[0063] Indoor culture and oil accumulation of embodiment 3ENN2203A algae
[0064] The green swimming cells of Scenedesmus strain ENN2203A in the logarithmic growth phase were inoculated in the prepared BG11 medium (see Table 1 for the formula) to make the cell density reach OD 750 between 0.2-0.8; the light intensity during the cultivation process is controlled at 50-500umol / m 2 .s, during the culture period, adjust the pH value of the culture medium between 7-9 and the temperature within the range of 15-35°C by feeding a mixture of 1.5-2% carbon dioxide and air into the culture medium The reactor used for cultivating is a column reactor with an internal diameter of 40mm and a length of 600mm; image 3 On the 16th day of cultivation, the final dry weight reached 10.93g / L. At this time, the color of the algae liquid turned yellow, and the algae liquid was collected, and the algae mud was obtained by centrifugation or natural sedimentation, and the algae mud was vacuum freeze-d...
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