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A technology of carotene and determination method, which is applied in the field of content determination of strawberry carotenoid components, and achieves the effects of high precision, simple and fast method, and good repeatability
Inactive Publication Date: 2013-06-26
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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Determination of strawberry carotenoid conte...
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Embodiment 1
[0053] (1) Preparation of reference substance solution: Accurately weigh 1.0mg of lutein, β-carotene, β-cryptoxanthin, and lycopene reference substance respectively, add acetone to a 5ml volumetric flask, and obtain a concentration of 200ug / ml of a single reference substance solution; respectively, accurately measure a single reference substance solution and put it in the same volumetric flask to obtain a mixed reference substance solution.
[0054] (2) Preparation of the test solution: Accurately weigh 10 g of ripe strawberry fruit pulp, quickly grind it to powder with liquid nitrogen, put it in a stoppered Erlenmeyer flask, and accurately add acetone:petroleum ether=1:2 extractant (containing 0.1 %BHT), at 50°C, leached in the dark for 3 hours, filtered out the supernatant, and repeatedly leached the residue until the residue turned white, combined the supernatant, steamed to a paste with a rotary evaporator at 40°C, and added 40ml of petroleum Ether (containing 0.1% BHT) a...
Embodiment 2
[0060] (1) Preparation of reference substance solution: Accurately weigh 1.0mg of lutein, β-carotene, β-cryptoxanthin, and lycopene reference substance respectively, add acetone to a 5ml volumetric flask, and obtain a concentration of 200ug / ml of a single reference substance solution; respectively, accurately measure a single reference substance solution and put it in the same volumetric flask to obtain a mixed reference substance solution.
[0061] (2) Preparation of the test solution: Accurately weigh 5 g of ripe strawberry fruit pulp, quickly grind it to powder with liquid nitrogen, put it in a stoppered Erlenmeyer flask, and accurately add acetone:petroleum ether=1:2 extractant (containing 0.1 %BHT), at 50°C, leaching in the dark for 3 hours, filtering out the supernatant, repeatedly leaching the residue until the residue turns white, combining the supernatant, steaming to a paste with a rotary evaporator at 40°C, adding 20ml of petroleum Ether (containing 0.1% BHT) and 1...
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Abstract
The invention provides a strawberry carotenoid component content determination method. According to the invention, with an ultra performance liquid chromatography method, content determinations upon 4 carotenoid components such as lutein, beta-carotene, beta-cryptoxanthin and lycopene in strawberry fruits are simultaneously carried out under a same chromatographic condition. According to the invention, acetone and petroleum ether with a ratio of 1:2 is adopted as an extraction agent, and carotenoids in strawberry fruits are subjected to saponification extraction; acetonitrile and methanol with a ratio of 1:9 are adopted as a mobile phase; a detection absorption wavelength is 450nm, and detection can be finished in 5min. The method provided by the invention has the advantages of high precision, high sensitivity, good repeatability, and high recovery rate. With the invention, separation and content determinations of the 4 carotenoid components in strawberry are simultaneously carried out. The method is simple and fast, the result is accurate and reliable, and reagent consumption is low. The method is an optimal method of qualitative and quantitative analysis of carotenoids of strawberry fruits.
Description
【Technical field】 [0001] The invention relates to a content determination method of strawberry carotenoids. 【Background technique】 [0002] Strawberry (Fragaria ananassa Duch) is a perennial herb of Rosaceae. The fruit is delicious, red and tender, rich and juicy, fragrant and nutritious. It is known as the "Queen of Fruits". In recent years, as the economic value of strawberries has become increasingly popular, people have begun to pay more and more attention to the fruit quality of strawberries. Carotenoids are not only an important indicator to determine the internal nutritional quality of fruits and vegetables, but also an important factor affecting the appearance quality of fruits and the ornamental value of flowers. More and more medical research shows that, in addition to many carotenoids are the precursors of vitamin A, many carotenoids can protect human health by quenching free radicals, enhancing human immunity, preventing cardiovascular diseases, and preventing c...
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