Silk-fibroin-based antibacterial nanofiber and preparation method thereof

A silk fibroin and nanofiber technology, applied in the field of medical materials, can solve problems such as poor mechanical properties of materials, and achieve the effects of good application prospects, lasting antibacterial effect, and excellent mechanical properties.

Inactive Publication Date: 2013-07-03
南京欧睿医药科技有限公司
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  • Application Information

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Problems solved by technology

Chinese patent application CN1348820A discloses a kind of antibacterial silk fibroin wound protective film and its preparation method, which adopts the method of blending silk fibroin and antibacterial agent to obtain antibacterial protective film; A

Method used

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  • Silk-fibroin-based antibacterial nanofiber and preparation method thereof

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Embodiment 1

[0030] A kind of silk fibroin-based antibacterial nanofiber, its preparation method comprises the steps:

[0031] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and obtained pure silk fibroin fiber after repeating 3 times; the silk fibroin was dissolved in LiBr:C 2 h 5 OH:H 2 O=45:44:11 (mass ratio) mixed solution, stirred and dissolved in a 70°C water bath to obtain a regenerated silk fibroin solution; after dialysis, filtration, and film formation, a regenerated silk fibroin film is formed;

[0032] (2) Prepare the regenerated silk fibroin film, titanium dioxide and chitosan in a mass ratio of 95:3:2, first dissolve the regenerated silk fibroin film and titanium dioxide in formic acid, and then add chitosan to the above solution , stirred at room temperature for 4h, and prepared into a spinning solution with a mass fraction of 10%;

[0033] (3) Spinning the above-prepared spinning solution at room temperature using an ele...

Embodiment 2

[0036] A kind of silk fibroin-based antibacterial nanofiber, its preparation method comprises the steps:

[0037] (1) Natural mulberry silk was degummed by boiling with 0.05% sodium carbonate solution for 30 minutes, and obtained pure mulberry silk fibroin fiber after repeated 3 times; the silk fibroin was dissolved in CaCl 2 :C 2 h 5 OH: H2 O=1:2:8 (molar ratio) mixed solution, stirring and dissolving in a 70°C water bath to obtain a regenerated silk fibroin solution; after dialysis, filtration, and film formation, a regenerated silk fibroin film is formed;

[0038] (2) Prepare the regenerated silk fibroin film, titanium dioxide and chitosan in a mass ratio of 70:15:15, first dissolve the regenerated silk fibroin film and titanium dioxide in formic acid, and then add chitosan to the above solution , stirred at room temperature for 6h, and prepared into a spinning solution with a mass fraction of 20%;

[0039] (3) Spinning the above-mentioned spinning solution at room tempe...

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Abstract

The invention discloses a silk-fibroin-based antibacterial nanofiber and a preparation method thereof. The nanofiber comprises the following components in parts by weight: 40-98 parts of silk fibroin, 1-10 parts of titanium dioxide and 1-60 parts of chitosan. The preparation method comprises the following steps: degumming and drying silk to obtain fibroin, processing fibroin to prepare a regenerated silk fibroin film, dissolving silk fibroin, titanium dioxide and chitosan in a polar solvent according to a certain mass ratio to prepare a spinning solution with the mass concentration of 0.01-80%, and preparing the silk fibroin-based antibacterial nanofiber with the average diameter of 10-1000 nm in a room-temperature state by electrostatic spinning equipment. The silk fibroin-based antibacterial nanofiber newly developed by the invention has an antibacterial ratio of more than 95%, has the characteristics of good biocompatibility and excellent mechanical performance while having the characteristic of long antibacterial property, and is suitable for biomedical materials.

Description

technical field [0001] The invention relates to a nanofiber material and a preparation method thereof, in particular to a silk fibroin-based antibacterial nanofiber material and a preparation method thereof, belonging to the field of medical materials. Background technique [0002] Nano antibacterial material is a new type of material with antibacterial properties. Due to the special effect of high specific surface area and high reactivity of the antibacterial agent in the material, the overall antibacterial effect is greatly improved, and it can make microorganisms including bacteria, fungi, yeast, Growth and reproduction of algae, viruses etc. are kept at a low level. Various products made of antibacterial materials have hygienic and self-cleaning functions, which can effectively prevent the spread of bacteria and strengthen the mechanical properties of antibacterial materials. Nano antibacterial products can not only effectively prevent people from people, people from thi...

Claims

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Application Information

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IPC IPC(8): D01F4/00D01F1/10
Inventor 明津法孙亦彬
Owner 南京欧睿医药科技有限公司
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