Preparation method of common bombax flower extract with whitening and anti-aging activities, and application thereof in cosmetics

A technology of kapok and extract, applied in the field of preparation of kapok extract

Active Publication Date: 2013-07-17
GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of kapok is mostly in food, medicine, health care and other industries, and there are few studies on it in cosmetics, especially the research on its application in whitening and anti-aging cosmetics has not been reported.

Method used

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  • Preparation method of common bombax flower extract with whitening and anti-aging activities, and application thereof in cosmetics
  • Preparation method of common bombax flower extract with whitening and anti-aging activities, and application thereof in cosmetics
  • Preparation method of common bombax flower extract with whitening and anti-aging activities, and application thereof in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of kapok extract with whitening and anti-aging activity

[0019] Accurately weigh 50 g of kapok after drying in natural environment, and crush it into a powder with an average particle size of 50 meshes; mix kapok powder with 750 mL of mass concentration of 70% ethanol aqueous solution, and add it to a round bottom flask, then mix it with 80 ℃ water bath leaching twice for 1.5h, combined the two extracts to obtain the crude extract; concentrated the kapok ethanol crude extract obtained under reduced pressure, recovered ethanol, and obtained the suspension; mixed petroleum ether with the suspension by volume Extract at a ratio of 2:1, combine the extracts, concentrate under reduced pressure to recover petroleum ether, and obtain a fine extract; extract ethyl acetate and the fine extract at a volume ratio of 2:1 until the ethyl acetate layer is basically colorless, and combine the extracted liquid, concentrated under reduced pressure to recover ethyl...

Embodiment 2

[0020] Example 2 Inhibition of tyrosinase activity test

[0021] (1) Materials

[0022] 1. Solution preparation

[0023] (1) HCl (0.1mol / L): 0.862mL of 36%-38% hydrochloric acid solution was dissolved in 99.138mL of water.

[0024] (2) L-tyrosine solution: 0.05 g was dissolved in 35 mL of 0.1 mol / L HCl, and the volume was adjusted to 100 mL with PBS (0.1 mol / L) buffer solution of pH 6.8.

[0025] (3) PBS solution (pH=6.8, 0.1 mol / L): ① Dissolve 17.91 g of disodium hydrogen phosphate dodecahydrate in deionized water, and dilute to 500 mL with deionized water. ② Dissolve 7.8g of sodium dihydrogen phosphate dodecahydrate in deionized water, and dilute to 500mL with deionized water. ③ Take 92.6mL and 107.4mL respectively to prepare 200mL of phosphate buffer solution with pH=6.8.

[0026] (4) Tyrosinase: 100 U / mL.

[0027] 2. Sample preparation

[0028] The above kapok extract was dissolved in dimethyl sulfoxide to prepare a sample solution with a concentration of 0.1%, and t...

Embodiment 3

[0039] Example 3 Inhibit melanin synthesis test in melanocytes

[0040] Seed B16 melanoma cells in a 96-well plate at a density of 1×105 / mL, 90 μL per well, CO 2 After 24 hours of incubation in the incubator, the sample solution was added to each well. At the same time, a blank control group of culture medium and cells was set up.

[0041] After the culture plate was incubated in an incubator for 72 hours, the supernatant was discarded, washed twice with PBS, and then 0.5 mL of trypsin was added to each well to digest the cells for 3 min, and 2 mL of maintenance solution was added to each well to stop the digestion. After mixing, take out 0.5mL of each concentration for cell counting. The rest of the cell suspension was centrifuged at 2500r / min for 5min, the supernatant was discarded, NaOH solution was added to the precipitate, heated to dissolve the melanin, and the absorbance value was measured with an enzyme-linked immunosorbent assay at a wavelength of 490nm. Calculate ...

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Abstract

The invention discloses a preparation method of a common bombax flower extract with whitening and anti-aging activities. The preparation method is characterized in that dried common bombax flowers are crushed and sieved; the common bombax flower is subjected to ethanol soaking extraction twice; extracted liquids obtained in the two times are combined; reduced-pressure concentration is carried out, such that a suspension liquid is obtained; the suspension liquid is sequentially extracted by using petroleum ether and ethyl acetate; reduced-pressure concentration is carried out, and ethyl acetate is recovered; and a paste is obtained by vacuum drying, such that the common bombax flower extract is obtained. With the method, the prepared common bombax flower extract has anti-free radical oxidation, tyrosinase activity inhibiting, and anti-ultraviolet radiation functions, and can be used in skin whitening and aging-preventing especially photoaging-preventing. The common bombax flower extract can be used as a cosmetic additive or a medicine active component, and can be applied in whitening and anti-aging cosmetics.

Description

technical field [0001] The invention relates to a preparation method of kapok extract. The extract has whitening and anti-aging effects, can be applied to whitening and anti-aging cosmetics, and belongs to the field of fine chemicals. Background technique [0002] Skin whitening and anti-aging is the theme of cosmetics, and cosmetics with skin whitening and anti-aging functions are one of the mainstream cosmetics favored by consumers. Modern scientific research has proved that skin aging is a very complicated process, which is affected by many factors, among which the generation of melanin and the degeneration of skin elastic fibers caused by free radicals are the two main factors. [0003] The color of human skin depends on the content and distribution of melanin. When a large amount of melanin is deposited unevenly in the human body, freckles and chloasma will form. Tyrosinase widely exists in the human body and is the key enzyme for producing melanin. The formation proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/08A61Q19/02
Inventor 胡兴国孟祥艳刘畅孙永张凯李丰孔胜仲
Owner GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD
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