Preparation method of photosensitizer-doped nanoparticles with fluorescence resonance energy transfer and antenna effects and used for photodynamic therapy

A technology of fluorescence resonance energy and photodynamic therapy, which is applied in the field of photosensitizers, can solve few problems in the field of photodynamic therapy, and achieve the effects of high singlet oxygen production, enhanced fluorescence emission, and high fluorescence enhancement

Inactive Publication Date: 2013-07-24
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the one hand, fluorescence resonance energy transfer (FRET) is an effective way to improve the luminescence efficiency of acceptors through energy transfer between donors and acceptors, and it is widely used to design highly efficient fluorescent nanoparticles for biological applications. Imaging field, but rarely used in photodynamic therapy field
On the other hand, the antenna effect is also increasingly applied to enhance fluorescence emission, but according to the existing technology, there is no research on applying the antenna effect to the field of photodynamic therapy

Method used

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  • Preparation method of photosensitizer-doped nanoparticles with fluorescence resonance energy transfer and antenna effects and used for photodynamic therapy
  • Preparation method of photosensitizer-doped nanoparticles with fluorescence resonance energy transfer and antenna effects and used for photodynamic therapy
  • Preparation method of photosensitizer-doped nanoparticles with fluorescence resonance energy transfer and antenna effects and used for photodynamic therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Prepare a tetrahydrofuran solution of 5,10,15,20-tetrakis(4-pyridine)porphyrin at a concentration of 6 mM;

[0030] 2) configuring a tetrahydrofuran solution of perylene at a concentration of 6 mM;

[0031] 3) Mix the solutions in step 1) and step 2) into a 10ml tea volumetric flask at a volume ratio of 0.2:100;

[0032] 4) Take 200 μl of the mixed solution in step 3) and add it into a round-bottomed flask filled with 5ml of high-purity water, and stir it with ultrasound at 1200 rpm for 5 minutes to obtain the final aqueous solution containing nanoparticles.

Embodiment 2

[0034] 1) Prepare tetrahydrofuran solution of tetrasubstituted phenylporphyrin with 6mM concentration;

[0035] 2) configuring a tetrahydrofuran solution of perylene at a concentration of 6 mM;

[0036] 3) Mix the solutions in step 1) and step 2) into a 10ml tea volumetric flask at a volume ratio of 0.2:100;

[0037] 4) Take 150 μl of the mixed solution in step 3) and add it to a round-bottomed flask filled with 5ml of high-purity water, and stir for 8 minutes with ultrasound at 1400 rpm to obtain the final aqueous solution containing nanoparticles.

Embodiment 3

[0039] 1) Prepare a tetrahydrofuran solution of 5,10,15,20-tetrakis(4-pyridine)porphyrin at a concentration of 6 mM;

[0040] 2) Acetone solution of pyrene with a concentration of 6mM is configured;

[0041] 3) Mix the solutions in step 1) and step 2) into a 10ml tea volumetric flask at a volume ratio of 0.2:100;

[0042] 4) Add 300 μl of the mixed solution in step 3) into a round-bottomed flask filled with 5 ml of high-purity water, and stir for 10 minutes with ultrasound at 1500 rpm to obtain the final aqueous solution containing nanoparticles.

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Abstract

The invention discloses a preparation method of photosensitizer-doped nanoparticles with fluorescence resonance energy transfer and antenna effects and used for photodynamic therapy. The method comprises the following steps: 1) preparing a certain concentration of organic solvent solution A of porphyins; 2) preparing a certain concentration of organic solvent solution B of perylene or pyrene; 3) according to certain doping proportion, mixing the solution A and the solution B to obtain a solution C; and 4) dropwise adding a certain amount of solution C into water, severely stirring to obtain a final solution D, namely a nanoparticle-containing aqueous solution. The preparation method is simple and feasible, and excellent nanoparticles integrating diagnosis and treatment can be prepared and applied for cancer therapy.

Description

technical field [0001] The invention relates to the field of photosensitizers for photodynamic therapy, in particular to a method for preparing photosensitizer-doped nanoparticles for photodynamic therapy with fluorescence resonance energy transfer and antenna effect. Background technique [0002] Photodynamic therapy (PDT) is one of the most potential cancer treatment modes, because it is not only a non-invasive technology, but also has high selectivity for cancer treatment. [0003] Photodynamic therapy uses light on the lesion to generate singlet oxygen that can kill disease cells through the energy conversion of photosensitizers. Therefore, the generation of singlet oxygen is a key indicator to measure the efficiency of photosensitizers. On the other hand, since photodynamic therapy is a treatment method under the action of external stimuli, accurate diagnosis and positioning before phototherapy are essential. Interestingly, most of the photosensitizers have the potent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61P35/00
Inventor 张晓宏张金凤安菲菲
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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