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Bacillus siamensis and application of bacillus siamensis in preparation of microbial seaweed fertilizer

A technology of Bacillus simimus and microorganisms, which is applied to the field of Bacillus simimus and its application in microbial seaweed fertilizers, can solve the problems affecting the sustainable development of tobacco leaf production, the decline of intrinsic quality of tobacco leaves, and the ecological imbalance of tobacco fields. Good control effect, improvement of product quality, outstanding effect of growth-promoting ability

Active Publication Date: 2013-07-24
青岛海大生物集团股份有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The long-term use of chemical fertilizers in the production of tobacco leaves in various parts of my country has resulted in poor soil, soil compaction, and nutritional imbalances, resulting in a decline in the intrinsic quality of tobacco leaves and poor aroma quality
Coupled with successive crop rotations, pests and diseases have become more serious, and drug resistance has increased, and the ecological imbalance of tobacco fields has even seriously affected the sustainable development of tobacco leaf production.

Method used

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  • Bacillus siamensis and application of bacillus siamensis in preparation of microbial seaweed fertilizer
  • Bacillus siamensis and application of bacillus siamensis in preparation of microbial seaweed fertilizer
  • Bacillus siamensis and application of bacillus siamensis in preparation of microbial seaweed fertilizer

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Embodiment 1

[0021] 1. Isolation and screening of Bacillus simum

[0022] On July 4, 2012, a total of more than 50 samples of rhizosphere soil were sampled from tobacco planting soil in Yishui and Zhucheng tobacco areas in Shandong, and 2g samples were suspended in 20ml saline, 180rpm, and shaken for 30min. Take 100 μL of the sample suspension and evenly spread it on the nutrient agar plate, culture it upside down in a 30°C incubator for 16 hours, pick single colonies with different shapes and inoculate them on the nutrient agar plate by streaking, and obtain pure A total of 88 pure bacteria were screened out.

[0023] The obtained strain was inoculated into the liquid NB medium with an inoculation loop, cultivated at 28°C and 180rpm for 64h, and the cultured fermentation broth was centrifuged at 10000rpm for 10min to remove most of the bacteria, and the supernatant was collected, and the obtained supernatant was subjected to anti- TMV biological activity assay. Choose healthy, vigorousl...

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Abstract

The invention provides bacillus siamensis and application of the bacillus siamensis in the preparation of a microbial seaweed fertilizer. The bacillus siamensis L13 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on 6th March 2013 under the CGMCC No: 7285. The screened high-activity bacillus siamensis fermentation liquor provided by the invention is used for preparing a disinfectant and a promoter, and also can be applied to preparing the efficient microbial seaweed fertilizer. The microbial seaweed fertilizer is not only complete in nutrition, but also prominent in stress resistance, disease and insect resistance, and growth promoting capacity, can replace a farm chemical, a fertilizer, and a hormone, improves soil properties and product quality and causes the product to reach the green or organic standard.

Description

technical field [0001] The invention relates to the field of microorganisms and microbial fertilizers, in particular to a strain of Bacillus sime and its application in microbial seaweed fertilizers. Background technique [0002] The long-term use of chemical fertilizers in the production of tobacco leaves in various parts of our country has caused poor soil, soil compaction, and nutritional imbalance, resulting in a decline in the intrinsic quality of tobacco leaves and the deterioration of aroma quality. Coupled with successive crop rotations, pests and diseases have become more serious, and drug resistance has increased, leading to an ecological imbalance in tobacco fields and even seriously affecting the sustainable development of tobacco leaf production. Problems such as agricultural soil erosion, soil productivity decline, agricultural products and agricultural environmental pollution, and ecological environment damage caused by the rapid development of agriculture hav...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00A01P21/00C05F11/08C12R1/07
Inventor 翟熙伦单俊伟宋海妹乔来艳张术聪
Owner 青岛海大生物集团股份有限公司
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