Bacillus siamensis and application of bacillus siamensis in preparation of microbial seaweed fertilizer
A technology of Bacillus simimus and microorganisms, which is applied to the field of Bacillus simimus and its application in microbial seaweed fertilizers, can solve the problems affecting the sustainable development of tobacco leaf production, the decline of intrinsic quality of tobacco leaves, and the ecological imbalance of tobacco fields. Good control effect, improvement of product quality, outstanding effect of growth-promoting ability
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[0021] 1. Isolation and screening of Bacillus simum
[0022] On July 4, 2012, a total of more than 50 samples of rhizosphere soil were sampled from tobacco planting soil in Yishui and Zhucheng tobacco areas in Shandong, and 2g samples were suspended in 20ml saline, 180rpm, and shaken for 30min. Take 100 μL of the sample suspension and evenly spread it on the nutrient agar plate, culture it upside down in a 30°C incubator for 16 hours, pick single colonies with different shapes and inoculate them on the nutrient agar plate by streaking, and obtain pure A total of 88 pure bacteria were screened out.
[0023] The obtained strain was inoculated into the liquid NB medium with an inoculation loop, cultivated at 28°C and 180rpm for 64h, and the cultured fermentation broth was centrifuged at 10000rpm for 10min to remove most of the bacteria, and the supernatant was collected, and the obtained supernatant was subjected to anti- TMV biological activity assay. Choose healthy, vigorousl...
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