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Tree-type high-molecular polyamide-amine wrapped platinum nanometer particles, its preparation method and applications

A dendrimer and platinum nanoparticle technology, which is applied in the fields of polymer chemistry and biomaterials, can solve the problems of large dosage, repeated use, small molecular substances without continuous reduction characteristics, etc., and achieve low cytotoxicity and stability Good, effective and safe removal effect

Inactive Publication Date: 2013-08-28
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, due to the immunogenicity of exogenous enzymes, it will cause the immune response of the organism, so the removal of excessively high reactive oxygen species in cells mainly depends on small molecular reducing substances, but small molecular substances do not have continuous reduction properties, so more Used once, and with a large amount

Method used

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  • Tree-type high-molecular polyamide-amine wrapped platinum nanometer particles, its preparation method and applications
  • Tree-type high-molecular polyamide-amine wrapped platinum nanometer particles, its preparation method and applications
  • Tree-type high-molecular polyamide-amine wrapped platinum nanometer particles, its preparation method and applications

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Preparation of polyamide-amine dendrimer-wrapped platinum nanoparticles

[0035] Taking the 9th generation dendrimer Ac-G9 with acetyl groups on the surface as an example, the preparation process is as follows: figure 1 shown.

[0036] Preparation method: Take 1 milliliter of 3.917 micromoles of the 9th generation acetylated polyamide-amine dendrimer aqueous solution, and add 0.01 moles per liter of 0.802 milliliters of potassium chloroplatinate aqueous solution dropwise to the solution, and the dendrimer height The ratio of molecule to platinum is 1: 2048. After the dropwise addition is completed, the reaction solution is stirred and reacted for 12 hours at 25 degrees Celsius, and then 40 microliters of 1M sodium borohydride aqueous solution is added dropwise to the reaction solution. The reaction time is 10 minutes, and finally dialyzed with water 8 times, the dialysate is double-distilled water, and the cut-off molecular weight of the dialysis bag for dia...

Embodiment 2

[0040] Example 2: Ac-G9 / Pt NP Catalyzed Hydrogen Peroxide Decomposition

[0041] The platinum nanoparticle Ac-G9 / Pt NP coated with the polyamide-amine dendrimer of the present invention prepared in Example 1 was used as a catalase mimetic enzyme to catalyze the decomposition of hydrogen peroxide, which was detected by an ultraviolet-visible spectrometer. The decomposition rate of hydrogen oxide.

[0042] The specific method is: in a round bottom flask at 37 degrees Celsius, add a reaction solution with a volume of 10mL, wherein the concentration of hydrogen peroxide is 5-75mM, the concentration of Ac-G9 / Pt NP is 10nM, and the concentration of catalase is 2.5nM . The absorbance at 240 nm was detected by spectrophotometer within 10 minutes. Record the absorption value at 2 minutes, 5 minutes and 10 minutes respectively, so as to obtain the reaction rate curve, the initial velocity v 0 It can be obtained by calculating the slope at 0 minutes. The apparent kinetic parameters c...

Embodiment 3

[0046] Example 3: Ac-G9 / Pt NP catalyzes the removal of diphenylpicrylphenylhydrazine DPPH free radical

[0047] The Ac-G9 / Pt NP (as catalase analog enzyme) prepared in Example 1 is reacted with DPPH free radicals, and free radical scavenging will make the solution turn from red to yellow, which can be calculated by detecting the absorption at 517nm Obtain DPPH free radical scavenging efficiency. The specific method is as follows: prepare an ethanol solution of 0.06 mmol per liter of DPPH, take 1.9 ml of the solution and add it to an aqueous solution of 0.1 ml of Ac-G9 / Pt NP. After 60 minutes, measure the absorbance of the solution at 517 nm. Add 0.1 ml of double distilled water to the blank control group. The percentage of absorbance decrease is the scavenging efficiency of DPPH free radicals.

[0048] Experimental results: Ac-G9 / Pt NP acts as a catalase mimic enzyme to scavenge DPPH free radicals, such as Figure 4 shown. Ac-G9 / Pt NPs can scavenge 40% of DPPH free radica...

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Abstract

The invention discloses tree-type high-molecular polyamide-amine wrapped platinum nanometer particles, and its preparation method, wherein the tree-type high-molecular polyamide-amine is shown as a formula (1). The tree-type high-molecular polyamide-amine and potassium chloroplatinate are used as raw materials, a sodium borohydride solution is used in a reduction reaction, and the tree-type high-molecular polyamide-amine wrapped platinum nanometer particles are omitted. The invention further provides application of the tree-type high-molecular polyamide-amine wrapped platinum nanometer particles as a catalase simulation enzyme. The tree-type high-molecular polyamide-amine wrapped platinum nanometer particles has small cytotoxicity, can effectively and safely remove active oxygen in a cell, can be used as the catalase simulation enzyme, and has advantages of high efficiency and low toxicity.

Description

technical field [0001] The invention relates to the technical fields of polymer chemistry and biomaterials, in particular to a platinum nanoparticle wrapped by polyamide-amine dendrimers and its preparation method and application. Background technique [0002] Reactive oxygen molecules, including hydrogen peroxide, superoxide radicals, and hydroxyl radicals, are by-products of cellular metabolism. It plays a very important role in many biological processes. However, excessive production of reactive oxygen species will destroy the protective mechanism of cells and lead to damage of cell structure, which will lead to a series of diseases, such as diabetes, inflammation, neurodegenerative diseases and cardiovascular diseases. Usually, cells have their own antioxidant system to remove intracellular reactive oxygen species, such as catalase, superoxide dismutase, peroxisomes, etc. However, some cells will lose their antioxidant function under pathological conditions, which will...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/24A61K47/18A61P39/06B22F9/24B22F1/02
Inventor 张银聪王辛宇程义云
Owner EAST CHINA NORMAL UNIV
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