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Lactic acid bacteria strain for high yield of amylase and application thereof

A technology of amylase and lactic acid bacteria, applied in the field of lactic acid bacteria

Active Publication Date: 2013-09-11
JINZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to aim at the slow growth of lactic acid bacteria in the starch matrix and weak acid production ability, which restricts the development of lactic acid bacteria fermented drinks with starch as the main matrix , to provide a lactic acid bacterium with high amylase production, which can grow rapidly and produce acid in substrates with high starch content such as purple potato and rice milk

Method used

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  • Lactic acid bacteria strain for high yield of amylase and application thereof
  • Lactic acid bacteria strain for high yield of amylase and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Isolation, screening and identification of Lactobacillus paracasei LHY006

[0029] 1 Separation medium: use soluble starch instead of glucose in MRS medium as carbon source, the specific components and their proportions are as follows: peptone 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, diammonium citrate 2g, sodium acetate 5g, soluble Starch 20g, Tween 80 1mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, calcium carbonate 5g, distilled water 1000mL. Adjust the pH to 6.2-6.4, and sterilize at 115°C for 30 minutes.

[0030] 2 detection method

[0031] 2.1 Detection method of amylase activity

[0032] 1% substrate solution: Weigh 1.0g of sweet potato starch, dissolve in 25mL 0.4mol / L NaOH solution, heat in a boiling water bath for 10min, add 25mL 0.4mol / L CH 3 COOH solution, adjust the pH to 7.0, and then dilute it in a 100mL volumetric flask.

[0033] 0.005% iodine solution: Weigh 0.5g of iodine and 5.0g of KI, dissolve in distilled water and set th...

Embodiment 2

[0068] Application of Lactobacillus paracasei LHY006 in purple sweet potato rice milk fermented beverage

[0069] 1. Test materials

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Abstract

The invention discloses a lactic acid bacteria strain for the high yield of amylase and an application thereof. The lactic acid bacteria strain is Lactobacillus paracasei (Lactobacillus paracasei) LHY006, is collected in the China General Microbiological Culture Collection Center on April, 17, 2013 and has the collection number of CGMCC No.7480. The lactic acid bacteria strain is applied to purple sweet potato rice milk fermented beverages; the lactic acid bacteria strain comprises the following main raw materials in parts by weight: 8 parts of purple sweet potato powder, 7 parts of rice meal, 1.8 parts of inulin, 2 parts of dried skim milk, 0.2 part of a stabilizer, 8 parts of white granulated sugar, 3 parts of leavening agent and 150 parts of purified water; moreover, the purple sweet potato rice milk fermented beverage is a beverage with excellent nutrition and health care effects.

Description

Technical field [0001] The present invention involves a lactic acid bacteria and it is used in purple potato rice slurry fermented beverages. technical background [0002] Lactic acid bacteria can improve the flavor, quality and nutrition of food, extend the shelf life, and have an important physiological and health care role of regulating the balancing of human intestinal flora, inhibiting the growth of pathogenic bacteria, reducing cholesterol, improving immunity, and delaying aging., Lactic bacteria fermented foods are also considered functional foods.At present, the most common foods rich in lactic acid bacteria on the market are sour milk. This kind of breast milk cholesterol with animal milk as the base is high, and it is not suitable for long -term drinking of middle -aged and elderly people.But it can make up for this defect. [0003] Purple potato is a new variety of sweet potato species introduced from Japan. Because it contains very rich anthocyanins, selenium, polysac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/38A23L2/62C12R1/225
Inventor 张莉力许云贺于洋
Owner JINZHOU MEDICAL UNIV
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