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Biosurfactant and preparation method thereof

A biological surface and active agent technology, applied in chemical instruments and methods, drilling compositions, chemical/physical processes, etc., can solve the problem of high cost of surfactants, achieve low price, avoid huge damage, and good emulsification performance Effect

Inactive Publication Date: 2013-10-02
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the raw materials used in the above preparation process are relatively expensive octadecylamine and methyl acrylate, and if the prepared surfactant is used in oil recovery, the cost will be too high

Method used

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  • Biosurfactant and preparation method thereof
  • Biosurfactant and preparation method thereof
  • Biosurfactant and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] The enzyme-catalyzed hydrolysis of embodiment 1 protein

[0049] Accurately weigh 15g of protein powder, dissolve in 250mL of deionized water, stir and heat to 50°C, and preheat for 10min. Adjust the pH value to 9.5±0.05 with 2M NaOH, and add 200-400 μL of enzyme solution for hydrolysis. A 905Titrando automatic potentiometric titrator was used to add 1M NaOH solution dropwise to control the pH value of the reaction system to be 9.45-9.55. The hydrolysis reaction generally lasts for 30 minutes. After the end, the pH of the solution is adjusted to 4.0 with concentrated hydrochloric acid to inactivate the activity of alkaline protease Protex 6L. Protein polypeptides with different degrees of hydrolysis are prepared by controlling the amount of protease added and the hydrolysis time. The degree of hydrolysis of the protein polypeptide was calculated by measuring the concentration of free amino acids in the hydrolyzate by the OPA method, and a standard curve was drawn with...

Embodiment 2

[0053] The acid-catalyzed hydrolysis of embodiment 2 protein

[0054] Add 10 mL of 12.0 mol / L concentrated hydrochloric acid to 10 mL of 60 g / L protein aqueous solution, hydrolyze for 22 h at 110 °C under nitrogen protection conditions to completely hydrolyze the protein, and measure the concentration of amino acids in the hydrolyzed product by OPA method.

[0055] The protein powder selected here can be soybean protein isolate, corn gluten powder, wheat gluten powder, or fish scale collagen.

Embodiment 3

[0056] The preparation of embodiment 3 lipopeptide surfactants

[0057] Get 20ml of one of the protein hydrolyzates prepared in the above-mentioned examples 1 and 2, put it into a reactor with a temperature control jacket, and drip the total molar amount of free amino groups with a constant current pump under 800rpm magnetic stirring. 1.5 times of the acylating reagent was added dropwise within 1 hour. During this process, an automatic potentiometric titrator was used to maintain the reaction at a constant pH value of 9.5±0.05, and the reaction temperature was controlled at 4°C. After the dropwise addition of the acylating reagent was completed, the reaction temperature was raised to 50° C., and the reaction was continued for 2 h before ending.

[0058] The acylating reagents selected here include: oleoyl chloride, lauroyl chloride, decanoyl chloride, or benzoyl chloride.

[0059] The acylation rate of lipopeptide surfactants was determined by OPA method.

[0060] figure 1...

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Abstract

The invention relates to a biosurfactant having the general structure shown in formula (I). The preparation method of the biosurfactant comprises following steps: taking a mass-produced protein which is one selected from soybean isolate protein, corn protein powder, wheat protein powder or fish scale collagen protein as a raw material; performing hydrochloric acid hydrolysis or enzyme catalytic hydrolysis so as to obtain polypeptides with different hydrolysis degrees; and then subjecting the polypeptides to acylation reaction with oleoyl chloride, lauroyl chloride, decanoyl chloride, or benzoyl chloride. The biosurfactant possesses excellent emulsifying property and surface and interfacial activity, can be used alone as an oil displacement agent, and can also be combined with other surfactants and then used in tertiary oil recovery.

Description

technical field [0001] The invention relates to a surfactant and a preparation method thereof, in particular to a lipopeptide biosurfactant for oil displacement and a preparation method thereof. Background technique [0002] For a long time, oil has been the main energy source for human survival, and thus is an extremely important strategic resource, which plays an important role in the country's economic development and the improvement of people's living standards, and is called the blood of industry. In recent years, although the whole world is actively developing and applying new energy sources, the proportion of petroleum consumption in total energy is increasing year by year. Petroleum is a non-renewable fossil energy. my country's crude oil resources are relatively scarce, and most of the oil fields currently need to be further exploited. Therefore, how to improve oil recovery and make up for the shortage of crude oil has important practical significance for the stable...

Claims

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Application Information

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IPC IPC(8): B01F17/30C09K8/584C12P21/06C09K23/30
Inventor 张松平苏志国刘祥玉张羽飞张贵锋王平
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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