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Method for extracting androstenedione by use of membrane method

An androstenedione-membrane technology is applied in the field of membrane integration technology to extract androstenedione, which can solve the problems of low yield of androstenedione, rough process and low quality, avoid interface emulsification, be easy to automate, The effect of short production cycle

Active Publication Date: 2015-03-25
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the characteristics of low yield, low quality, rough process and difficult extraction of androstenedione, the present invention uses membrane integration technology to extract androstenedione, which can effectively solve the current extraction and purification problems of androstenedione. Disadvantages, provide a more efficient extraction method for androstenedione production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] With sunflower oil 160g / L, phytosterol 5 g / L, NH 4 NO 3 5.4g / L, K 2 HPO 4 0.6g / L is used as the medium, and the microscopic mycobacteria strains are added to the medium, the inoculum size is 10% (v / v), and after 7 days of aerobic fermentation, it is allowed to stand for stratification.

[0028] The oil-phase fermentation liquid is treated with an alumina ceramic membrane with a pore size of 200nm, an operating pressure of 0.25MPa, and a flow velocity of 3m / s on the membrane surface to remove bacterial impurities and residual water in the fermentation liquid.

[0029] The filtrate of the ceramic membrane is directly extracted with methanol, and the methanol is added from the bottom up to make the extraction more complete. During the extraction process, the layered interface is clear and the operator's operation time is short.

[0030] The methanol extract is concentrated through a polyethersulfone organic membrane with a molecular weight of 150. The operating pr...

Embodiment 2

[0033] With sunflower oil 160g / L, phytosterol 5 g / L, NH 4 NO 3 5.4g / L, K 2 HPO 4 0.6g / L is used as the medium, and the microscopic mycobacteria strains are added to the medium, the inoculum size is 10% (v / v), and after 7 days of aerobic fermentation, it is allowed to stand for stratification.

[0034] The oil-phase fermentation liquid is treated with a zirconia ceramic membrane. The pore size of the ceramic membrane is 100nm, the operating pressure is 0.15MPa, and the membrane surface flow rate is 2m / s to remove bacterial impurities and residual water in the fermentation liquid.

[0035] The filtrate of the ceramic membrane is directly extracted with methanol, and methanol is charged from the bottom up to make the extraction more complete. During the extraction process, the layered interface is clear, and the worker's operation time is short.

[0036] Concentrate the methanol extract through a polyacrylonitrile organic membrane with a molecular weight of 100, control...

Embodiment 3

[0039] With sunflower oil 160g / L, phytosterol 5 g / L, NH 4 NO 3 5.4g / L, K 2 HPO 4 0.6g / L is used as the medium, and the microscopic mycobacteria strains are added to the medium, the inoculum size is 10% (v / v), and after 7 days of aerobic fermentation, it is allowed to stand for stratification.

[0040] The oil-phase fermentation liquid is treated with a titanium oxide ceramic membrane. The ceramic membrane has a pore size of 50nm, an operating pressure of 0.15MPa, and a flow velocity of 4m / s on the membrane surface to remove bacterial impurities and residual water in the fermentation liquid.

[0041] The filtrate of the ceramic membrane is directly extracted with methanol, and methanol is charged from the bottom up to make the extraction more complete. During the extraction process, the layered interface is clear, and the worker's operation time is short.

[0042]The methanol extract is concentrated through a polysulfone organic membrane with a molecular weight of 300...

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Abstract

The invention provides a method for extracting androstenedione by use of membrane integration technology. The method for extracting the androstenedione comprises the following steps: preparing the androstenedione by a fermentation method to obtain an androstenedione containing oil phase liquid and an aqueous phase; standing for layering, and separating two-phase material liquid; through ceramic membrane treatment of an oil phase material liquid, separating an androstenedione containing oil phase from impurities such as bacteria slag and the like, wherein a penetration side being a ceramic membrane purification liquid; extracting the ceramic membrane purification liquid with methanol to obtain a methanol extract liquor; sending the methanol extract liquor to a nanofiltration membrane for condensation, and obtaining a nanofiltration membrane concentrated liquor at an interception side; and pulping the nanofiltration membrane concentrated liquor, and obtaining androstenedione dry powder after crystallization deposition, centrifugation and drying. According to the method for extracting the androstenedione, the extracted androstenedione has high yield and purity, a post-extraction process can be speeded up, and the whole technology has strong operability and low environmental pollution. Finished products can fully meet technical requirements for exporting to nations such as Spain and the like, and can achieve the requirements of industrial production.

Description

technical field [0001] The invention relates to a method for extracting androstenedione, in particular to a method for extracting androstenedione by membrane integration technology. Background technique [0002] Androstenedione scientific name: Δ4-androstenedione-3,17 dione. It is an intermediate in the biosynthesis of testosterone from progesterone via androsterone, and its biological activity is weaker than that of testosterone. It can also be converted into estrone in the body, and finally excreted in the urine in the form of more polar compounds - 17-ketosteroids. As a simplified hormone drug, a steroid compound with androgen effect extracted from the testis or urine, an intermediate of norethindrone and testosterone propionate, is widely used in rheumatoid arthritis, contraception, diuresis and various A kind of control of infectious inflammation, etc., is a C19 steroid compound formed from 17α-hydroxyprogesterone by the action of C17-C20 lyase, which is used as an in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J1/00
Inventor 王贝贝陈磊彭文博熊福军张宏范克银
Owner JIANGSU JIUWU HITECH