Pathogenic strain for Scophthalmus maximus and application thereof

Abstract

The objective of the invention is to provide a pathogenic strain separated from tissue of Scophthalmus maximus with an edge-rot disease. The pathogenic strain is Vibrio anguillarum which is preserved in China General Microbiological Culture Collection Center, No. 3, Yard 1, West Beichen Road, Chaoyang District, Beijing on June 17th, 2013, with an accession number of CGMCC No. 7733. The screened Vibrio anguillarum is used for preparation of an inactivated vaccine. A preparation method for the inactivated vaccine for the Scophthalmus maximus edge-rot disease has the advantages of simple process, stable output, low cost and capacity of obtaining a great number of the inactivated vaccine with high immune effects. The inactivated vaccine prepared by using the preparation method has an immune protective rate of 68.5%, is a purely biological preparation, has the characteristics of safety and environment friendliness, does not generate drug residue, poses no pollution to the environment, fundamentally suppresses occurrence and prevalence of the Scophthalmus maximus edge-rot disease and provides technical support for large scale preparation of the inactivated vaccine for the Scophthalmus maximus edge-rot disease.

Description

technical field [0001] The invention belongs to the field of microbial vaccine preparation, and in particular relates to a turbot pathogenic strain and an inactivated vaccine for preventing edge rot prepared by the strain. Background technique [0002] Turbot (Scophthalmas maximus) is a rare seawater fish species in Europe and one of the important seawater cultured fishes in Europe. It was introduced to my country for artificial breeding in 1992. It has reached a certain scale and has gradually become an excellent seawater fish in northern my country. It is the main object of aquaculture species and factory farming, with an annual output value of more than 1.5 billion yuan. However, with the expansion of the breeding range, the increase of the breeding density, and the degeneration of the species, the disease problem of turbot has become increasingly prominent, and various bacterial diseases, parasitic diseases, and viral diseases have occurred from time to time, and they are...

AI Technical Summary

Problems solved by technology

Since 2009, there has been an outbreak of epidemic diseases in turbot in Weihai breeding area in Shandong Province. The first symptoms are that the side fins and tail fins become lighter in color, and bleeding spots appear. Lesions, diseased turbot will die without festering. The disease mostly occurs in the seedling stage and early growing stage. It is highly contagious, widespread and has a high mortality rate. It only takes 3 to 4 days for fins to die from bleeding spots, which is an acute death and causes great economic losses to the breeding owners.

[0003] At present, the treatment of bacterial diseases of turbot mainly relies on antibiotics. Although they have certain effects, they cannot fundamentally control the disease, and the fish are prone to drug resistance, resulting in drug residues in the environment and destroying the ecological balance. , a threat to human health and safety

In addition, fish schools are prone to anorexia symptoms during illness, so the method of feeding bait is not ideal